FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005732211

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005732211
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3169174
Sequences flagged as poor quality0
Sequence length51
%GC55

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TCGTACGACTCTTAGCGGTGGAATTCTCGGGTGCCAAGGAACTCCAGTCAC778572.456696918503055RNA PCR Primer, Index 1 (100% over 33bp)
TGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCGATGTATCTCGTATGCC460271.452334267541006RNA PCR Primer, Index 2 (100% over 51bp)
GGAATACCGGGTGCTGTAGGCTTTGGAATTCTCGGGTGCCAAGGAACTCCA378151.1932131211476555RNA PCR Primer, Index 1 (100% over 28bp)
GTACGACTCTTAGCGGTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCG336641.06223261960372RNA PCR Primer, Index 2 (100% over 35bp)
ACACAAGGGTTTGTTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCGAT321131.0132924225681519RNA PCR Primer, Index 2 (100% over 37bp)
GGAATACCGGGTGCTGTAGGCTTTTGGAATTCTCGGGTGCCAAGGAACTCC320411.0110205372125356RNA PCR Primer, Index 1 (100% over 27bp)
TCCTTCATTCCACCGGAGTCTGTGGAATTCTCGGGTGCCAAGGAACTCCAG313240.9883963455461896RNA PCR Primer, Index 1 (100% over 29bp)
AATACCGGGTGCTGTAGGCTTTGGAATTCTCGGGTGCCAAGGAACTCCAGT292940.9243418001031184RNA PCR Primer, Index 1 (100% over 30bp)
CCACGTTCCCGTGGTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCGAT291560.9199873531715204RNA PCR Primer, Index 2 (100% over 37bp)
GAATACCGGGTGCTGTAGGCTTTGGAATTCTCGGGTGCCAAGGAACTCCAG281890.8894746706870623RNA PCR Primer, Index 1 (100% over 29bp)
GAATACCGGGTGCTGTAGGCTTTTGGAATTCTCGGGTGCCAAGGAACTCCA214400.6765169725613046RNA PCR Primer, Index 1 (100% over 28bp)
CCTGTCTGAGCGTCGCTTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACC170780.5388785847668826RNA PCR Primer, Index 2 (100% over 34bp)
AATACCGGGTGCTGTAGGCTTTTGGAATTCTCGGGTGCCAAGGAACTCCAG168660.5321891445531233RNA PCR Primer, Index 1 (100% over 29bp)
GAGACCGCCTGGGAATACCGGGTGCTGTAGGCTTTGGAATTCTCGGGTGCC158500.5001303178683152No Hit
GTCTACGGCCATACCACCCTGAACGCGCCCGATGGAATTCTCGGGTGCCAA137300.4332359157307235No Hit
AGACCGCCTGGGAATACCGGGTGCTGTAGGCTTTGGAATTCTCGGGTGCCA129910.4099175368723838No Hit
CGCGACCTCAGATCAGACGTGGCGACCCGCTGATGGAATTCTCGGGTGCCA121410.38309666809080223No Hit
TTTCGGCACCATGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCGATGTA109650.3459892072824023RNA PCR Primer, Index 2 (100% over 40bp)
GTCTACGGCCATACCACCCTGAACGCGCCCGTGGAATTCTCGGGTGCCAAG107870.3403726018199064No Hit
GTCTACGGCCATACCACCCTGAACGCGCCCGATCTGGAATTCTCGGGTGCC107720.3398992923708197No Hit
GTCTACGGCCATACCACCCTGAACGCGCCCGATTGGAATTCTCGGGTGCCA106940.3374380832355686No Hit
ATACCGGGTGCTGTAGGCTTTGGAATTCTCGGGTGCCAAGGAACTCCAGTC103530.32667818175966357RNA PCR Primer, Index 1 (100% over 31bp)
ACACAAGGGTTTGTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCGATG100580.3173697625942911RNA PCR Primer, Index 2 (100% over 38bp)
CCCCACGTTGGGCGCCATGGAATTCTCGGGTGCCAAGGAACTCCAGTCACC99710.3146245677895881RNA PCR Primer, Index 2 (100% over 34bp)
ACCACGTTCCCGTGGTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCGA98370.3103963367110799RNA PCR Primer, Index 2 (100% over 36bp)
TCTGAGCGTCGCTTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCGATG94740.298942248043181RNA PCR Primer, Index 2 (100% over 38bp)
CGACACAAGGGTTTGTTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCG94680.29875292426354627RNA PCR Primer, Index 2 (100% over 35bp)
TTCACAGTGGCTAAGTTCTGTGGAATTCTCGGGTGCCAAGGAACTCCAGTC88590.2795365606306249RNA PCR Primer, Index 1 (100% over 31bp)
CCTGGGAATACCGGGTGCTGTAGGCTTTGGAATTCTCGGGTGCCAAGGAAC87690.27669670393610446RNA PCR Primer, Index 1 (100% over 24bp)
TTCGGGCGCCATGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCGATGTA86260.2721844871881443RNA PCR Primer, Index 2 (100% over 40bp)
ATGGGAGACCGCCTGGGAATACCGGGTGCTGTAGGCTTTGGAATTCTCGGG85320.2692184146405341No Hit
GGGAATACCGGGTGCTGTAGGCTTTGGAATTCTCGGGTGCCAAGGAACTCC85270.26906064482417186RNA PCR Primer, Index 1 (100% over 27bp)
TAGCTTATCAGACTGATGTTGATGGAATTCTCGGGTGCCAAGGAACTCCAG82820.2613299238224219RNA PCR Primer, Index 1 (100% over 29bp)
GGTGCTGTAGGCTTTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCGAT81390.25681770707446167RNA PCR Primer, Index 2 (100% over 37bp)
GGGCTACGCCTGTCTGAGCGTCGCTTGGAATTCTCGGGTGCCAAGGAACTC79400.25053846838324434RNA PCR Primer, Index 1 (100% over 26bp)
GGAATACCGGGTGCTGTAGGCTTGGAATTCTCGGGTGCCAAGGAACTCCAG79380.25047536045669944RNA PCR Primer, Index 1 (100% over 29bp)
GTCTGAGCGTCGCTTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCGAT77960.24599469767201168RNA PCR Primer, Index 2 (100% over 37bp)
CCGGGTGCTGTAGGCTTTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACC76050.23996789068697397RNA PCR Primer, Index 2 (100% over 34bp)
TAGCTTATCAGACTGATGTTGACTGGAATTCTCGGGTGCCAAGGAACTCCA73860.23305757273030764RNA PCR Primer, Index 1 (100% over 28bp)
TGGGAATACCGGGTGCTGTAGGCTTTGGAATTCTCGGGTGCCAAGGAACTC73520.23198473797904437RNA PCR Primer, Index 1 (100% over 26bp)
ACCGCCTGGGAATACCGGGTGCTGTAGGCTTTGGAATTCTCGGGTGCCAAG73220.23103811908087094No Hit
AATACCGGGTGCTGTAGGCTTGGAATTCTCGGGTGCCAAGGAACTCCAGTC72330.22822981634962294RNA PCR Primer, Index 1 (100% over 31bp)
ACCGGGTGCTGTAGGCTTTGGAATTCTCGGGTGCCAAGGAACTCCAGTCAC71070.22425401697729438RNA PCR Primer, Index 1 (100% over 33bp)
CTGTCTGAGCGTCGCTTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCG70940.22384381545475254RNA PCR Primer, Index 2 (100% over 35bp)
GAATACCGGGTGCTGTAGGCTTGGAATTCTCGGGTGCCAAGGAACTCCAGT70940.22384381545475254RNA PCR Primer, Index 1 (100% over 30bp)
CGGGTGCTGTAGGCTTTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCG69030.21781700846971483RNA PCR Primer, Index 2 (100% over 35bp)
TCGACACAAGGGTTTGTTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACC67770.21384120909738627RNA PCR Primer, Index 2 (100% over 34bp)
GTTTCGGCACCATGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCGATGT67700.2136203313544791RNA PCR Primer, Index 2 (100% over 39bp)
GGGAATACCGGGTGCTGTAGGCTTTTGGAATTCTCGGGTGCCAAGGAACTC66660.21033871917414443RNA PCR Primer, Index 1 (100% over 26bp)
TACCGGGTGCTGTAGGCTTTGGAATTCTCGGGTGCCAAGGAACTCCAGTCA66230.20898189875342912RNA PCR Primer, Index 1 (100% over 32bp)
CACCACGTTCCCGTGGTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCG66080.20850858930434238RNA PCR Primer, Index 2 (100% over 35bp)
TTCGCGCTTTCCCCTGTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCG64570.2037439408502026RNA PCR Primer, Index 2 (100% over 35bp)
CCTGGGAATACCGGGTGCTGTAGGCTTTTGGAATTCTCGGGTGCCAAGGAA63740.20112496189858936RNA PCR Primer, Index 1 (100% over 23bp)
ATACCGGGTGCTGTAGGCTTTTGGAATTCTCGGGTGCCAAGGAACTCCAGT62160.1961394357015424RNA PCR Primer, Index 1 (100% over 30bp)
TTTCCCGGCCAATGCACCATGGAATTCTCGGGTGCCAAGGAACTCCAGTCA61810.19503504698700672RNA PCR Primer, Index 1 (100% over 32bp)
TTAGTACTTGGATGGGAGACCGCCTGGAATTCTCGGGTGCCAAGGAACTCC61750.194845723207372RNA PCR Primer, Index 1 (100% over 27bp)
TGGGAATACCGGGTGCTGTAGGCTTTTGGAATTCTCGGGTGCCAAGGAACT60520.1909645857248608RNA PCR Primer, Index 1 (100% over 25bp)
TACTTGGATGGGAGACCGCCTGGAATTCTCGGGTGCCAAGGAACTCCAGTC59440.18755675769143632RNA PCR Primer, Index 1 (100% over 31bp)
CAAGGGTTTGTTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCGATGTA58690.18519021044600265RNA PCR Primer, Index 2 (100% over 40bp)
CGCTTTCCCCTGTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCGATGT57990.1829814330169312RNA PCR Primer, Index 2 (100% over 39bp)
GGTGCTGTAGGCTTTTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCGA57760.1822556918616649RNA PCR Primer, Index 2 (100% over 36bp)
CACAAGGGTTTGTTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCGATG55710.17578712939081287RNA PCR Primer, Index 2 (100% over 38bp)
CGCCTGTCTGAGCGTCGCTTGGAATTCTCGGGTGCCAAGGAACTCCAGTCA55110.17389389159446594RNA PCR Primer, Index 1 (100% over 32bp)
TACTTGGATGGGAGACCGCCTGGGAATACCGGGTGCTGTAGGCTTTGGAAT54430.1717482220919394No Hit
TGTCTGAGCGTCGCTTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCGA53830.16985498429559248RNA PCR Primer, Index 2 (100% over 36bp)
GCCTGTCTGAGCGTCGCTTGGAATTCTCGGGTGCCAAGGAACTCCAGTCAC53720.16950789069959554RNA PCR Primer, Index 1 (100% over 33bp)
AATTCTCGGGTGCCAAGGAACTCCAGTCACCGATGTATCTCGTATGCCGTC52970.16714134345416187RNA PCR Primer, Index 2 (100% over 51bp)
CTCGACACAAGGGTTTGTTGGAATTCTCGGGTGCCAAGGAACTCCAGTCAC52630.1660685087028986RNA PCR Primer, Index 1 (100% over 33bp)
CCGCCTGGGAATACCGGGTGCTGTAGGCTTTGGAATTCTCGGGTGCCAAGG52360.1652165516945425Illumina Small RNA Adapter 2 (100% over 21bp)
TACCCTGTAGAACCGAATTTGTTGGAATTCTCGGGTGCCAAGGAACTCCAG51400.1621873712203874RNA PCR Primer, Index 1 (100% over 29bp)
TCCCTGTTCGGGCGCCATGGAATTCTCGGGTGCCAAGGAACTCCAGTCACC50150.1582431258113313RNA PCR Primer, Index 2 (100% over 34bp)
TCCTTCATTCCACCGGAGTCTGTTGGAATTCTCGGGTGCCAAGGAACTCCA49700.15682319746407108RNA PCR Primer, Index 1 (100% over 28bp)
CGTTCGCGCTTTCCCCTGTGGAATTCTCGGGTGCCAAGGAACTCCAGTCAC49650.15666542764770885RNA PCR Primer, Index 1 (100% over 33bp)
CTACGCCTGTCTGAGCGTCGCTTGGAATTCTCGGGTGCCAAGGAACTCCAG48100.15177456334047926RNA PCR Primer, Index 1 (100% over 29bp)
CCGGGTGCTGTAGGCTTTTGGAATTCTCGGGTGCCAAGGAACTCCAGTCAC46470.1466312673270701RNA PCR Primer, Index 1 (100% over 33bp)
GGGGCTACGCCTGTCTGAGCGTCGCTTGGAATTCTCGGGTGCCAAGGAACT44690.14101466186457418RNA PCR Primer, Index 1 (100% over 25bp)
GTCTACGGCCATACCACCCTGAACGCGCCCGATCTCGTCTGATGGAATTCT44380.14003648900312826No Hit
CTCGGGTGCCAAGGAACTCCAGTCACCGATGTATCTCGTATGCCGTCTTCT44290.1397525033336762RNA PCR Primer, Index 2 (100% over 51bp)
ACCGGGTGCTGTAGGCTTTTGGAATTCTCGGGTGCCAAGGAACTCCAGTCA44110.13918453199477213RNA PCR Primer, Index 1 (100% over 32bp)
AATACCGGGTGCTGTAGGCTTATGGAATTCTCGGGTGCCAAGGAACTCCAG43430.13703886249224562RNA PCR Primer, Index 1 (100% over 29bp)
CGCGACCTCAGATCAGACGTGGCTGGAATTCTCGGGTGCCAAGGAACTCCA43310.13666021493297623RNA PCR Primer, Index 1 (100% over 28bp)
GTCTACGGCCATACCACCCTGAACGCGCCCTGGAATTCTCGGGTGCCAAGG43220.1363762292635242Illumina Small RNA Adapter 2 (100% over 21bp)
GCATTGGTGGTTCAGTGGTAGAATTCTCGCCTGCTGGAATTCTCGGGTGCC42730.13483008506317418No Hit
TTGTACGACTCTTAGCGGTGGAATTCTCGGGTGCCAAGGAACTCCAGTCAC42620.13448299146717724RNA PCR Primer, Index 1 (100% over 33bp)
CATTATTACTTTTGGTACGCGTGGAATTCTCGGGTGCCAAGGAACTCCAGT42480.13404123598136297RNA PCR Primer, Index 1 (100% over 30bp)
CGGGTGCTGTAGGCTTTTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACC42260.1333470487893691RNA PCR Primer, Index 2 (100% over 34bp)
GTTAGTACTTGGATGGGAGACCGCCTGGAATTCTCGGGTGCCAAGGAACTC41250.13016009849885177RNA PCR Primer, Index 1 (100% over 26bp)
GATGGGAGACCGCCTGGGAATACCGGGTGCTGTAGGCTTTGGAATTCTCGG40770.1286455082617742No Hit
TACCGGGTGCTGTAGGCTTTTGGAATTCTCGGGTGCCAAGGAACTCCAGTC40480.1277304433268732RNA PCR Primer, Index 1 (100% over 31bp)
CTGGGAATACCGGGTGCTGTAGGCTTTGGAATTCTCGGGTGCCAAGGAACT40430.12757267351051094RNA PCR Primer, Index 1 (100% over 25bp)
CAGGGTCGGGCCTGGTTAGTACTTGGATGGGAGACCGCCTGGAATTCTCGG40230.12694159424506196No Hit
AACATTCAACGCTGTCGGTGAGTGGAATTCTCGGGTGCCAAGGAACTCCAG39890.1258687594937987RNA PCR Primer, Index 1 (100% over 29bp)
GTCTACGGCCATACCACCCTGAACGCGCCCGATCTCGTCTGTGGAATTCTC39270.12391241377090687No Hit
CCACGTTGGGCGCCATGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCGA39110.12340755035854768RNA PCR Primer, Index 2 (100% over 36bp)
TTAGTACTTGGATGGGAGACCGCCTGGGAATACCGGGTGCTGTAGGCTTTG38030.11999972232512321No Hit
ACAAGGGTTTGTTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCGATGT37790.11924242720658443RNA PCR Primer, Index 2 (100% over 39bp)
TATTGCACTTGTCCCGGCCTGTTGGAATTCTCGGGTGCCAAGGAACTCCAG37740.1190846573902222RNA PCR Primer, Index 1 (100% over 29bp)
TGAGGTAGTAGGTTGTATAGTTTGGAATTCTCGGGTGCCAAGGAACTCCAG36980.11668655618151606RNA PCR Primer, Index 1 (100% over 29bp)
CTGGGAATACCGGGTGCTGTAGGCTTTTGGAATTCTCGGGTGCCAAGGAAC36700.1158030452098875RNA PCR Primer, Index 1 (100% over 24bp)
GACCGCCTGGGAATACCGGGTGCTGTAGGCTTTGGAATTCTCGGGTGCCAA36550.11532973576080076No Hit
CACGTTGGGCGCCATGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCGAT36420.11491953423825893RNA PCR Primer, Index 2 (100% over 37bp)
CTAAGCAGGGTCGGGCCTGGTTAGTACTTGGATGGGAGACCGCCTGGAATT36040.11372048363390587No Hit
TCCAGGGTTCAGGTCCCTGTTCGGGCGCCATGGAATTCTCGGGTGCCAAGG36020.11365737570736097Illumina Small RNA Adapter 2 (100% over 21bp)
GTCTACGGCCATACCACCCTGAACGCGCCCGATCTCGTCTGGAATTCTCGG35370.1116063680946518No Hit
TTTCCCGGCCAACGCACCATGGAATTCTCGGGTGCCAAGGAACTCCAGTCA33770.10655773397105997RNA PCR Primer, Index 1 (100% over 32bp)
TCGTGGTTGTAGTCCGTGCGAGAATACCATGGAATTCTCGGGTGCCAAGGA33520.10576888488924875RNA PCR Primer, Index 1 (100% over 22bp)
ATACCGGGTGCTGTAGGCTTGGAATTCTCGGGTGCCAAGGAACTCCAGTCA33490.1056742229994314RNA PCR Primer, Index 1 (100% over 32bp)
AACGCACTTGCGGCCCCGGGTTCCTCCCGGGGCTACGCCTGTCTGAGCGTC32450.10239261081909672No Hit
TCTCGGGTGCCAAGGAACTCCAGTCACCGATGTATCTCGTATGCCGTCTTC32370.10214017911291712RNA PCR Primer, Index 2 (100% over 51bp)
CCCACGTTGGGCGCCATGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCG32260.10179308551692018RNA PCR Primer, Index 2 (100% over 35bp)
ATGGGAGACCGCCTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCGATG32030.10106734436165386RNA PCR Primer, Index 2 (100% over 38bp)
GGAATTCTCGGGTGCCAAGGAACTCCAGTCACCGATGTATCTCGTATGCCG31960.10084646661874672RNA PCR Primer, Index 2 (100% over 51bp)

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TACCTTA207.01578E-445.025211
TCTTTCA1800.045.025211
TATGTCT207.01578E-445.025211
AGTGCAT850.044.998223
CCATAGT207.036538E-444.998225
CGTCCAT207.036538E-444.998226
CTACGGT453.8562575E-1044.9982153
TTATTAG253.893274E-544.9982155
TTATCTA207.037085E-444.9975115
CCATACG302.1675405E-644.997519
GTAAGTT750.044.997518
CACCGTA253.8936367E-544.9975111
CATCACA551.8189894E-1244.997517
GATAGGG406.8248482E-944.9975115
TTGTACC650.044.99750512
GTCTACG108100.044.7961271
TGAGGTA16700.044.75561
ACCCGTA8450.044.745372
AGCTTAT24050.044.730972
TCCTTCA48250.044.605291
Produced by FastQC (version 0.11.5)