FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005732220

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005732220
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4550445
Sequences flagged as poor quality0
Sequence length51
%GC53

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TGGAATTCTCGGGTGCCAAGGAACTCCAGTCACTTAGGCATCTCGTATGCC60085113.204225081283258RNA PCR Primer, Index 3 (100% over 51bp)
GCATTGGTGGTTCAGTGGTAGAATTCTCGCCTGCTGGAATTCTCGGGTGCC1446603.1790297432448913No Hit
GCATTGGTGGTTCAGTGGTAGAATTCTCGCCTTGGAATTCTCGGGTGCCAA1215822.6718705533195104No Hit
TCGTACGACTCTTAGCGGTGGAATTCTCGGGTGCCAAGGAACTCCAGTCAC891961.9601599404014334RNA PCR Primer, Index 1 (100% over 33bp)
TCCTTCATTCCACCGGAGTCTGTGGAATTCTCGGGTGCCAAGGAACTCCAG713161.5672313367154203RNA PCR Primer, Index 1 (100% over 29bp)
GTTTCCGTAGTGTAGTGGTTATCACGTTCGCCTTGGAATTCTCGGGTGCCA689701.5156759393861479No Hit
TACCCTGTAGAACCGAATTTGTTGGAATTCTCGGGTGCCAAGGAACTCCAG648031.424102477889525RNA PCR Primer, Index 1 (100% over 29bp)
GCATTGTGGTTCAGTGGTAGAATTCTCGCCTGCTGGAATTCTCGGGTGCCA369630.8122941822173435No Hit
GTACGACTCTTAGCGGTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACTT328750.7224568146631813RNA PCR Primer, Index 1 (97% over 35bp)
GGAATACCGGGTGCTGTAGGCTTTTGGAATTCTCGGGTGCCAAGGAACTCC310820.6830540749311331RNA PCR Primer, Index 1 (100% over 27bp)
TGAGATGAAGCACTGTAGCTTGGAATTCTCGGGTGCCAAGGAACTCCAGTC293340.6446402494701068RNA PCR Primer, Index 1 (100% over 31bp)
GCATTGTGGTTCAGTGGTAGAATTCTCGCCTTGGAATTCTCGGGTGCCAAG292960.6438051663079105No Hit
TAGCTTATCAGACTGATGTTGACTGGAATTCTCGGGTGCCAAGGAACTCCA290480.6383551498809457RNA PCR Primer, Index 1 (100% over 28bp)
GGAATACCGGGTGCTGTAGGCTTTGGAATTCTCGGGTGCCAAGGAACTCCA282390.6205766688752419RNA PCR Primer, Index 1 (100% over 28bp)
TCCCTGGTGGTCTAGTGGTTAGGATTCGGCGCTTGGAATTCTCGGGTGCCA276100.6067538449536255No Hit
TAGCTTATCAGACTGATGTTGATGGAATTCTCGGGTGCCAAGGAACTCCAG274360.6029300431056743RNA PCR Primer, Index 1 (100% over 29bp)
GCATTGGTGGTTCAGTGGTAGAATTCTCGCCTGGAATTCTCGGGTGCCAAG258920.5689992956732803No Hit
TTCAAGTAATCCAGGATAGGCTTGGAATTCTCGGGTGCCAAGGAACTCCAG258260.5675488880757816RNA PCR Primer, Index 1 (100% over 29bp)
GCATGGGTGGTTCAGTGGTAGAATTCTCGCCTTGGAATTCTCGGGTGCCAA250400.5502758521419334No Hit
GCATTGGTGGTTCAGTGGTAGAATTCTCGCCTGTGGAATTCTCGGGTGCCA231190.5080602007056453No Hit
CCACGTTCCCGTGGTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACTTAG217350.47764559290355113RNA PCR Primer, Index 3 (100% over 37bp)
GAATACCGGGTGCTGTAGGCTTTGGAATTCTCGGGTGCCAAGGAACTCCAG184040.40444396097524526RNA PCR Primer, Index 1 (100% over 29bp)
TCCCTGGTGGTCTAGTGGTTAGGATTCGGCGCTCTGGAATTCTCGGGTGCC175680.3860721314069283No Hit
GAATACCGGGTGCTGTAGGCTTTTGGAATTCTCGGGTGCCAAGGAACTCCA173910.3821824019409091RNA PCR Primer, Index 1 (100% over 28bp)
AACATTCAACGCTGTCGGTGAGTGGAATTCTCGGGTGCCAAGGAACTCCAG168340.3699418408529276RNA PCR Primer, Index 1 (100% over 29bp)
TGAGAACTGAATTCCATAGGCTGTTGGAATTCTCGGGTGCCAAGGAACTCC168050.3693045405449357RNA PCR Primer, Index 1 (100% over 27bp)
TTCACAGTGGCTAAGTTCTGTGGAATTCTCGGGTGCCAAGGAACTCCAGTC162580.35728373818384795RNA PCR Primer, Index 1 (100% over 31bp)
GCATGGGTGGTTCAGTGGTAGAATTCTCGCCTGCTGGAATTCTCGGGTGCC155770.34231816888238403No Hit
GCCCGGCTAGCTCAGTCGGTAGAGCATGGGACTCTGGAATTCTCGGGTGCC154490.3395052571781441No Hit
TCCTTCATTCCACCGGAGTCTGTTGGAATTCTCGGGTGCCAAGGAACTCCA150570.3308907150839094RNA PCR Primer, Index 1 (100% over 28bp)
AAGCTGCCAGTTGAAGAACTGTTGGAATTCTCGGGTGCCAAGGAACTCCAG149050.32755038243512447RNA PCR Primer, Index 1 (100% over 29bp)
GGAATTCTCGGGTGCCAAGGAACTCCAGTCACTTAGGCATCTCGTATGCCG147830.3248693259670208RNA PCR Primer, Index 3 (100% over 51bp)
CCTGTCTGAGCGTCGCTTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACT136200.2993113860292784RNA PCR Primer, Index 3 (100% over 34bp)
AATACCGGGTGCTGTAGGCTTTGGAATTCTCGGGTGCCAAGGAACTCCAGT133550.29348777976659424RNA PCR Primer, Index 1 (100% over 30bp)
TGGGAATACCGGGTGCTGTAGGCTTTTGGAATTCTCGGGTGCCAAGGAACT130800.2874444147770163RNA PCR Primer, Index 1 (100% over 25bp)
GCATTTGTGGTTCAGTGGTAGAATTCTCGCCTGCTGGAATTCTCGGGTGCC129350.284257913237057No Hit
TCCCTGTGGTCTAGTGGTTAGGATTCGGCGCTTGGAATTCTCGGGTGCCAA127030.2791595107731222No Hit
GCATTTGTGGTTCAGTGGTAGAATTCTCGCCTTGGAATTCTCGGGTGCCAA126450.2778849101571385No Hit
TAGCTTATCAGACTGATGTTGACTTGGAATTCTCGGGTGCCAAGGAACTCC122390.2689627058452525RNA PCR Primer, Index 1 (100% over 27bp)
TGGGAATACCGGGTGCTGTAGGCTTTGGAATTCTCGGGTGCCAAGGAACTC120660.2651608798699907RNA PCR Primer, Index 1 (100% over 26bp)
AACATTCAACGCTGTCGGTGAGTTGGAATTCTCGGGTGCCAAGGAACTCCA120260.26428184496241575RNA PCR Primer, Index 1 (100% over 28bp)
GTTTCCGTAGTGTAGTGGTTATCACGTTCGCCTCTGGAATTCTCGGGTGCC119140.26182054722120585No Hit
ACACAAGGGTTTGTTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACTTAG114090.2507227315130718RNA PCR Primer, Index 3 (100% over 37bp)
TCAGTGCACTACAGAACTTTGTTGGAATTCTCGGGTGCCAAGGAACTCCAG113160.24867897535296RNA PCR Primer, Index 1 (100% over 29bp)
GCTTCTGTAGTGTAGTGGTTATCACGTTCGCCTTGGAATTCTCGGGTGCCA106370.23375735779687484No Hit
TTTGGCAATGGTAGAACTCACACTTGGAATTCTCGGGTGCCAAGGAACTCC105190.23116420481952862RNA PCR Primer, Index 1 (100% over 27bp)
GCCCGGCTAGCTCAGTCGGTAGAGCATGAGACTCTGGAATTCTCGGGTGCC104440.22951601436782557No Hit
TGAGGTAGTAGATTGTATAGTTTGGAATTCTCGGGTGCCAAGGAACTCCAG103440.2273184270988881RNA PCR Primer, Index 1 (100% over 29bp)
TGAGGTAGTAGGTTGTATAGTTTGGAATTCTCGGGTGCCAAGGAACTCCAG93410.20527662679144568RNA PCR Primer, Index 1 (100% over 29bp)
TCCCTGTGGTCTAGTGGTTAGGATTCGGCGCTCTGGAATTCTCGGGTGCCA91530.20114516272584332No Hit
TAGCTTATCAGACTGATGTTGTGGAATTCTCGGGTGCCAAGGAACTCCAGT91290.2006177417812983RNA PCR Primer, Index 1 (100% over 30bp)
GTCTGAGCGTCGCTTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACTTAG91220.2004639106724727RNA PCR Primer, Index 3 (100% over 37bp)
TCGACACAAGGGTTTGTTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACT90720.199365117038004RNA PCR Primer, Index 3 (100% over 34bp)
CGACACAAGGGTTTGTTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACTT90620.19914535831111022RNA PCR Primer, Index 1 (97% over 35bp)
TGAGGTAGTAGTTTGTGCTGTTTGGAATTCTCGGGTGCCAAGGAACTCCAG90580.19905745482035275RNA PCR Primer, Index 1 (100% over 29bp)
CAACGGAATCCCAAAAGCAGCTGTGGAATTCTCGGGTGCCAAGGAACTCCA87590.1924866688862298RNA PCR Primer, Index 1 (100% over 28bp)
GGGAATACCGGGTGCTGTAGGCTTTTGGAATTCTCGGGTGCCAAGGAACTC86070.18914633623744492RNA PCR Primer, Index 1 (100% over 26bp)
GCATTGGTGGTTCAGTGGTAGAATTCTCGCCTGCCTGGAATTCTCGGGTGC85400.18767395276725682No Hit
ATACCGGGTGCTGTAGGCTTTGGAATTCTCGGGTGCCAAGGAACTCCAGTC85070.18694874896850747RNA PCR Primer, Index 1 (100% over 31bp)
CAAGGGTTTGTTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACTTAGGCA85010.18681689373237123RNA PCR Primer, Index 3 (100% over 40bp)
AATACCGGGTGCTGTAGGCTTTTGGAATTCTCGGGTGCCAAGGAACTCCAG83140.18270740553945822RNA PCR Primer, Index 1 (100% over 29bp)
GGGAATACCGGGTGCTGTAGGCTTTGGAATTCTCGGGTGCCAAGGAACTCC80610.17714750974904653RNA PCR Primer, Index 1 (100% over 27bp)
CACCCGTAGAACCGACCTTGCGTGGAATTCTCGGGTGCCAAGGAACTCCAG78730.17301604568344414RNA PCR Primer, Index 1 (100% over 29bp)
TCAGTGCACTACAGAACTTTGTCTGGAATTCTCGGGTGCCAAGGAACTCCA71930.1580724522546696RNA PCR Primer, Index 1 (100% over 28bp)
ACCACGTTCCCGTGGTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACTTA68990.15161154568399354RNA PCR Primer, Index 3 (100% over 36bp)
TGTCTGAGCGTCGCTTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACTTA68900.1514137628297892RNA PCR Primer, Index 3 (100% over 36bp)
CTCGACACAAGGGTTTGTTGGAATTCTCGGGTGCCAAGGAACTCCAGTCAC68750.15108412473944854RNA PCR Primer, Index 1 (100% over 33bp)
GCATTGTGGTTCAGTGGTAGAATTCTCGCCTGGAATTCTCGGGTGCCAAGG68740.15106214886675917Illumina Small RNA Adapter 2 (100% over 21bp)
TCTGAGCGTCGCTTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACTTAGG66600.14635931211123307RNA PCR Primer, Index 3 (100% over 38bp)
TATTGCACTTGTCCCGGCCTGTTGGAATTCTCGGGTGCCAAGGAACTCCAG64640.1420520410641157RNA PCR Primer, Index 1 (100% over 29bp)
TGAGAACTGAATTCCATAGGCTGTGGAATTCTCGGGTGCCAAGGAACTCCA64000.14064558521199574RNA PCR Primer, Index 1 (100% over 28bp)
TGTAAACATCCTCGACTGGAAGCTTGGAATTCTCGGGTGCCAAGGAACTCC63760.14011816426745077RNA PCR Primer, Index 1 (100% over 27bp)
TCCCTGGTGGTCTAGTGGTTAGGATTCGGCGCTGGAATTCTCGGGTGCCAA63730.14005223664938263No Hit
GTCTACGGCCATACCACCCTGAACGCGCCCGATGGAATTCTCGGGTGCCAA62800.13800848048927084No Hit
TGAGATGAAGCACTGTAGCTCTTGGAATTCTCGGGTGCCAAGGAACTCCAG62760.13792057699851332RNA PCR Primer, Index 1 (100% over 29bp)
TAGCAGCACGTAAATATTGGCGTGGAATTCTCGGGTGCCAAGGAACTCCAG61720.1356350862388184RNA PCR Primer, Index 1 (100% over 29bp)
GGAATACCGGGTGCTGTAGGCTTGGAATTCTCGGGTGCCAAGGAACTCCAG61380.13488790656737967RNA PCR Primer, Index 1 (100% over 29bp)
GCATGGGTGGTTCAGTGGTAGAATTCTCGCCTGTGGAATTCTCGGGTGCCA61200.13449234085897094No Hit
TTCACAGTGGCTAAGTTCTGCTGGAATTCTCGGGTGCCAAGGAACTCCAGT60670.1333276196064341RNA PCR Primer, Index 1 (100% over 30bp)
TACCCTGTAGATCCGAATTTGTTGGAATTCTCGGGTGCCAAGGAACTCCAG59680.13115200821018602RNA PCR Primer, Index 1 (100% over 29bp)
ATACCGGGTGCTGTAGGCTTTTGGAATTCTCGGGTGCCAAGGAACTCCAGT59560.13088829773791355RNA PCR Primer, Index 1 (100% over 30bp)
CATTGCACTTGTCTCGGTCTGATGGAATTCTCGGGTGCCAAGGAACTCCAG58940.12952579363117234RNA PCR Primer, Index 1 (100% over 29bp)
ACACAAGGGTTTGTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACTTAGG58640.1288665174504911RNA PCR Primer, Index 3 (100% over 38bp)
TTGTACGACTCTTAGCGGTGGAATTCTCGGGTGCCAAGGAACTCCAGTCAC58040.12754796508912866RNA PCR Primer, Index 1 (100% over 33bp)
CTGTCTGAGCGTCGCTTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACTT58020.1275040133437499RNA PCR Primer, Index 1 (97% over 35bp)
TCGTGGTTGTAGTCCGTGCGAGAATACCATGGAATTCTCGGGTGCCAAGGA57760.12693264065382617RNA PCR Primer, Index 1 (100% over 22bp)
TACCGGGTGCTGTAGGCTTTGGAATTCTCGGGTGCCAAGGAACTCCAGTCA57120.12552618480170621RNA PCR Primer, Index 1 (100% over 32bp)
TCGCTGGTTCGTTTCCGGCTCGAAGGACCATGGAATTCTCGGGTGCCAAGG56890.1250207397298506Illumina Small RNA Adapter 2 (100% over 21bp)
CCGGGTGCTGTAGGCTTTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACT56840.12491086036640373RNA PCR Primer, Index 3 (100% over 34bp)
GAATACCGGGTGCTGTAGGCTTGGAATTCTCGGGTGCCAAGGAACTCCAGT55560.12209794866216381RNA PCR Primer, Index 1 (100% over 30bp)
GCATTGTGGTTCAGTGGTAGAATTCTCGCCTGTGGAATTCTCGGGTGCCAA54740.1202959271016351No Hit
CGGGTGCTGTAGGCTTTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACTT53880.11840600205034892RNA PCR Primer, Index 1 (97% over 35bp)
CACCACGTTCCCGTGGTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACTT53530.11763684650622083RNA PCR Primer, Index 1 (97% over 35bp)
CCTGGGAATACCGGGTGCTGTAGGCTTTGGAATTCTCGGGTGCCAAGGAAC53220.11695559445285021RNA PCR Primer, Index 1 (100% over 24bp)
TCCTTCATTCCACCGGAGTCTTGGAATTCTCGGGTGCCAAGGAACTCCAGT52610.11561506621879838RNA PCR Primer, Index 1 (100% over 30bp)
CATTATTACTTTTGGTACGCGTGGAATTCTCGGGTGCCAAGGAACTCCAGT52280.11488986242004903RNA PCR Primer, Index 1 (100% over 30bp)
AGGGTTTGTTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACTTAGGCATC52220.11475800718391278RNA PCR Primer, Index 3 (100% over 42bp)
CTGGGAATACCGGGTGCTGTAGGCTTTTGGAATTCTCGGGTGCCAAGGAAC52010.11429651385743592RNA PCR Primer, Index 1 (100% over 24bp)
GTAGTCGTGGCCTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACTTAGGC51500.11317574435027783RNA PCR Primer, Index 3 (100% over 39bp)
ACCGGGTGCTGTAGGCTTTGGAATTCTCGGGTGCCAAGGAACTCCAGTCAC51480.1131317926048991RNA PCR Primer, Index 1 (100% over 33bp)
GCCCGGCTAGCTCAGTCGGTAGAGCATGGGACTTGGAATTCTCGGGTGCCA51030.11214287833387723No Hit
AATACCGGGTGCTGTAGGCTTGGAATTCTCGGGTGCCAAGGAACTCCAGTC50460.1108902535905829RNA PCR Primer, Index 1 (100% over 31bp)
CATGGAATTCTCGGGTGCCAAGGAACTCCAGTCACTTAGGCATCTCGTATG50000.10987936344687167RNA PCR Primer, Index 3 (100% over 49bp)
TACCCTGTAGAACCGAATTTGTGTGGAATTCTCGGGTGCCAAGGAACTCCA49810.10946182186577356RNA PCR Primer, Index 1 (100% over 28bp)
TGGGGGGCCCAAGTCCTTCTGATCGAGGCCTGGAATTCTCGGGTGCCAAGG49610.10902230441198607Illumina Small RNA Adapter 2 (100% over 21bp)
GAGACCGCCTGGGAATACCGGGTGCTGTAGGCTTTGGAATTCTCGGGTGCC48150.10581382699933743No Hit
GCCCGGCTAGCTCAGTCGGTAGAGCATGAGACTTGGAATTCTCGGGTGCCA45710.1004517140631301No Hit
GGGGCTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACTTAGGCATCTCGT45560.10012207597278946RNA PCR Primer, Index 3 (100% over 46bp)

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CATCGGG206.8765535E-445.2092061
TCCTTCA109900.045.0652281
GTCTACG41550.045.0459941
CAACGGA11950.045.0200461
TTCGCCG1850.044.99658627
CCGTTTA351.2137207E-744.99658617
AGATAGT302.168057E-644.99658218
AACCCAC207.038122E-444.99658217
GCTCTAT207.038122E-444.99658218
TCAATTG406.8266672E-944.99658227
CAGATAT253.8943785E-544.99658217
TGAACGA302.1682226E-644.99608620
CAAACCC302.1683863E-644.99559415
CGTGATA3100.044.99559412
ATGCTAC406.828486E-944.99559413
ATCTTAG406.828486E-944.99559415
CCTGATA253.894884E-544.99559412
GATAGGG2850.044.99559415
GATAGAC207.038884E-444.99559412
AGACTAA253.89539E-544.99460610
Produced by FastQC (version 0.11.5)