FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005732221

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005732221
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2178802
Sequences flagged as poor quality0
Sequence length51
%GC56

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TGGAATTCTCGGGTGCCAAGGAACTCCAGTCACGGCTACATCTCGTATGCC1068204.9026942328857785RNA PCR Primer, Index 11 (100% over 51bp)
ACACAAGGGTTTGTTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACGGCT337601.549475353887136RNA PCR Primer, Index 11 (100% over 37bp)
TCTGAGCGTCGCTTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACGGCTA238031.0924810974104118RNA PCR Primer, Index 11 (100% over 38bp)
AATACCGGGTGCTGTAGGCTTTGGAATTCTCGGGTGCCAAGGAACTCCAGT225341.0342380812942158RNA PCR Primer, Index 1 (100% over 30bp)
CCTGTCTGAGCGTCGCTTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACG212410.974893542414593RNA PCR Primer, Index 6 (100% over 34bp)
TTTCGGCACCATGGAATTCTCGGGTGCCAAGGAACTCCAGTCACGGCTACA173340.7955748158850597RNA PCR Primer, Index 11 (100% over 40bp)
CGCTTTCCCCTGTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACGGCTAC167660.7695054438172904RNA PCR Primer, Index 11 (100% over 39bp)
GAATACCGGGTGCTGTAGGCTTTGGAATTCTCGGGTGCCAAGGAACTCCAG150000.6884517271417963RNA PCR Primer, Index 1 (100% over 29bp)
TTCGCGCTTTCCCCTGTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACGG134930.6192852769549504RNA PCR Primer, Index 2 (97% over 35bp)
GGAATACCGGGTGCTGTAGGCTTTGGAATTCTCGGGTGCCAAGGAACTCCA119330.5476862973322036RNA PCR Primer, Index 1 (100% over 28bp)
TCCTTCATTCCACCGGAGTCTGTGGAATTCTCGGGTGCCAAGGAACTCCAG118090.5419950963878315RNA PCR Primer, Index 1 (100% over 29bp)
AATACCGGGTGCTGTAGGCTTTTGGAATTCTCGGGTGCCAAGGAACTCCAG117860.540939470406214RNA PCR Primer, Index 1 (100% over 29bp)
TCGTACGACTCTTAGCGGTGGAATTCTCGGGTGCCAAGGAACTCCAGTCAC111360.5111065622300696RNA PCR Primer, Index 1 (100% over 33bp)
GGTGCTGTAGGCTTTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACGGCT100430.4609413797123373RNA PCR Primer, Index 11 (100% over 37bp)
GAATACCGGGTGCTGTAGGCTTTTGGAATTCTCGGGTGCCAAGGAACTCCA98790.45341430749558703RNA PCR Primer, Index 1 (100% over 28bp)
TTCGGGCGCCATGGAATTCTCGGGTGCCAAGGAACTCCAGTCACGGCTACA98690.4529553396774925RNA PCR Primer, Index 11 (100% over 40bp)
ACACAAGGGTTTGTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACGGCTA94610.43422945269923563RNA PCR Primer, Index 11 (100% over 38bp)
CAAGGGTTTGTTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACGGCTACA90200.4139889719212668RNA PCR Primer, Index 11 (100% over 40bp)
GGAATACCGGGTGCTGTAGGCTTTTGGAATTCTCGGGTGCCAAGGAACTCC86100.39517129137939105RNA PCR Primer, Index 1 (100% over 27bp)
CACAAGGGTTTGTTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACGGCTA84450.3875983223808313RNA PCR Primer, Index 11 (100% over 38bp)
GTCTGAGCGTCGCTTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACGGCT79740.3659809381485789RNA PCR Primer, Index 11 (100% over 37bp)
GTTTCGGCACCATGGAATTCTCGGGTGCCAAGGAACTCCAGTCACGGCTAC79690.36575145423953165RNA PCR Primer, Index 11 (100% over 39bp)
AGGGTTTGTTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACGGCTACATC78470.36015204685877833RNA PCR Primer, Index 11 (100% over 42bp)
ATACCGGGTGCTGTAGGCTTTGGAATTCTCGGGTGCCAAGGAACTCCAGTC78080.3583620723682097RNA PCR Primer, Index 1 (100% over 31bp)
AAGGGTTTGTTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACGGCTACAT76740.352211903605743RNA PCR Primer, Index 11 (100% over 41bp)
CTACGCCTGTCTGAGCGTCGCTTGGAATTCTCGGGTGCCAAGGAACTCCAG76670.35189062613307676RNA PCR Primer, Index 1 (100% over 29bp)
GCCTGTCTGAGCGTCGCTTGGAATTCTCGGGTGCCAAGGAACTCCAGTCAC73000.3350465072090075RNA PCR Primer, Index 1 (100% over 33bp)
CGCCTGTCTGAGCGTCGCTTGGAATTCTCGGGTGCCAAGGAACTCCAGTCA72720.33376139731834287RNA PCR Primer, Index 1 (100% over 32bp)
GGGCTACGCCTGTCTGAGCGTCGCTTGGAATTCTCGGGTGCCAAGGAACTC71870.32986017086453934RNA PCR Primer, Index 1 (100% over 26bp)
CGTTCGCGCTTTCCCCTGTGGAATTCTCGGGTGCCAAGGAACTCCAGTCAC71800.32953889339187314RNA PCR Primer, Index 1 (100% over 33bp)
TGTCTGAGCGTCGCTTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACGGC70720.3245820409564522RNA PCR Primer, Index 6 (97% over 36bp)
CCCCACGTTGGGCGCCATGGAATTCTCGGGTGCCAAGGAACTCCAGTCACG67780.311088387104473RNA PCR Primer, Index 6 (100% over 34bp)
AATACCGGGTGCTGTAGGCTTGGAATTCTCGGGTGCCAAGGAACTCCAGTC65410.3002108498156326RNA PCR Primer, Index 1 (100% over 31bp)
CCACGTTCCCGTGGTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACGGCT65340.2998895723429664RNA PCR Primer, Index 11 (100% over 37bp)
CGGGGCTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACGGCTACATCTCG63940.293464022889643RNA PCR Primer, Index 11 (100% over 45bp)
GGTGCTGTAGGCTTTTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACGGC60040.27556427798395633RNA PCR Primer, Index 6 (97% over 36bp)
TCCCTGTTCGGGCGCCATGGAATTCTCGGGTGCCAAGGAACTCCAGTCACG57970.26606364414939954RNA PCR Primer, Index 6 (100% over 34bp)
GTGCTGTAGGCTTTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACGGCTA55870.25642531996941437RNA PCR Primer, Index 11 (100% over 38bp)
TACCGGGTGCTGTAGGCTTTGGAATTCTCGGGTGCCAAGGAACTCCAGTCA55420.254359964787989RNA PCR Primer, Index 1 (100% over 32bp)
AATTCTCGGGTGCCAAGGAACTCCAGTCACGGCTACATCTCGTATGCCGTC51120.23462434860992415RNA PCR Primer, Index 11 (100% over 51bp)
CGACACAAGGGTTTGTTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACGG49980.22939211548364652RNA PCR Primer, Index 2 (97% over 35bp)
GTACGACTCTTAGCGGTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACGG48960.2247106437390823RNA PCR Primer, Index 2 (97% over 35bp)
TCACGGCACCATGGAATTCTCGGGTGCCAAGGAACTCCAGTCACGGCTACA45090.20694858917882394RNA PCR Primer, Index 11 (100% over 40bp)
ACAAGGGTTTGTTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACGGCTAC44850.20584706641539707RNA PCR Primer, Index 11 (100% over 39bp)
CCGGGTGCTGTAGGCTTTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACG43910.2015327689253085RNA PCR Primer, Index 6 (100% over 34bp)
CTCGACACAAGGGTTTGTTGGAATTCTCGGGTGCCAAGGAACTCCAGTCAC43730.20070662685273835RNA PCR Primer, Index 1 (100% over 33bp)
CACATTACCCGTCTGACCTGGAATTCTCGGGTGCCAAGGAACTCCAGTCAC43230.1984117877622657RNA PCR Primer, Index 1 (100% over 33bp)
ATACCGGGTGCTGTAGGCTTTTGGAATTCTCGGGTGCCAAGGAACTCCAGT43100.19781512959874278RNA PCR Primer, Index 1 (100% over 30bp)
GAATACCGGGTGCTGTAGGCTTGGAATTCTCGGGTGCCAAGGAACTCCAGT42550.19529080659922288RNA PCR Primer, Index 1 (100% over 30bp)
ACCGGGTGCTGTAGGCTTTGGAATTCTCGGGTGCCAAGGAACTCCAGTCAC41900.19230751578160843RNA PCR Primer, Index 1 (100% over 33bp)
GTGCTGTAGGCTTTTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACGGCT40150.18427557896495414RNA PCR Primer, Index 11 (100% over 37bp)
CTTTCCCCTGTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACGGCTACAT39310.18042024929296008RNA PCR Primer, Index 11 (100% over 41bp)
CTGAGCGTCGCTTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACGGCTAC38440.17642722927553764RNA PCR Primer, Index 11 (100% over 39bp)
TCGACACAAGGGTTTGTTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACG38350.17601415823925257RNA PCR Primer, Index 6 (100% over 34bp)
CTGTAGGCTTTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACGGCTACAT37400.17165396396735455RNA PCR Primer, Index 11 (100% over 41bp)
TCCTTCATTCCACCGGAGTCTGTTGGAATTCTCGGGTGCCAAGGAACTCCA37300.17119499614926RNA PCR Primer, Index 1 (100% over 28bp)
GGGGCTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACGGCTACATCTCGT35740.16403509818698533RNA PCR Primer, Index 11 (100% over 46bp)
CGGGTGCTGTAGGCTTTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACGG35060.1609141170239425RNA PCR Primer, Index 2 (97% over 35bp)
TATTGCACTTGTCCCGGCCTGTTGGAATTCTCGGGTGCCAAGGAACTCCAG34030.15618674849756886RNA PCR Primer, Index 1 (100% over 29bp)
CCTGGGAATACCGGGTGCTGTAGGCTTTGGAATTCTCGGGTGCCAAGGAAC33520.15384601262528674RNA PCR Primer, Index 1 (100% over 24bp)
AATACCGGGTGCTGTAGGCTTATGGAATTCTCGGGTGCCAAGGAACTCCAG32340.14843019237177127RNA PCR Primer, Index 1 (100% over 29bp)
TACCGGGTGCTGTAGGCTTTTGGAATTCTCGGGTGCCAAGGAACTCCAGTC31600.14503383051787175RNA PCR Primer, Index 1 (100% over 31bp)
GGAATTCTCGGGTGCCAAGGAACTCCAGTCACGGCTACATCTCGTATGCCG31020.14237181717292346RNA PCR Primer, Index 11 (100% over 51bp)
TACGGGCCACCATGGAATTCTCGGGTGCCAAGGAACTCCAGTCACGGCTAC29890.13718548082845528RNA PCR Primer, Index 11 (100% over 39bp)
CACCACGTTCCCGTGGTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACGG29630.1359921645014095RNA PCR Primer, Index 2 (97% over 35bp)
GGAATACCGGGTGCTGTAGGCTTGGAATTCTCGGGTGCCAAGGAACTCCAG29390.13489064173798263RNA PCR Primer, Index 1 (100% over 29bp)
GGGGCTACGCCTGTCTGAGCGTCGCTTGGAATTCTCGGGTGCCAAGGAACT29240.13420219001084083RNA PCR Primer, Index 1 (100% over 25bp)
TCCTTCATTCCACCGGACTCTGTGGAATTCTCGGGTGCCAAGGAACTCCAG29230.13415629322903136RNA PCR Primer, Index 1 (100% over 29bp)
TCCTTCTGATCGAGGCCTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACG28980.13300887368379505RNA PCR Primer, Index 6 (100% over 34bp)
AGTCACGGCACCATGGAATTCTCGGGTGCCAAGGAACTCCAGTCACGGCTA28820.13227452517484378RNA PCR Primer, Index 11 (100% over 38bp)
CACTTGGACGAACATGGAATTCTCGGGTGCCAAGGAACTCCAGTCACGGCT28410.1303927571206562RNA PCR Primer, Index 11 (100% over 37bp)
CTGTCTGAGCGTCGCTTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACGG28380.13025506677522786RNA PCR Primer, Index 2 (97% over 35bp)
GGTGCTGTAGGCTTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACGGCTA27920.12814381481199302RNA PCR Primer, Index 11 (100% over 38bp)
TTTCCCGGCCAATGCACCATGGAATTCTCGGGTGCCAAGGAACTCCAGTCA26850.12323285915838153RNA PCR Primer, Index 1 (100% over 32bp)
GTGGAACCTCCATGGAATTCTCGGGTGCCAAGGAACTCCAGTCACGGCTAC26430.12130519432238451RNA PCR Primer, Index 11 (100% over 39bp)
TACGCCTGTCTGAGCGTCGCTTGGAATTCTCGGGTGCCAAGGAACTCCAGT26340.12089212328609944RNA PCR Primer, Index 1 (100% over 30bp)
CTCGGGTGCCAAGGAACTCCAGTCACGGCTACATCTCGTATGCCGTCTTCT25600.1174957614321999RNA PCR Primer, Index 11 (100% over 51bp)
ATACCGGGTGCTGTAGGCTTGGAATTCTCGGGTGCCAAGGAACTCCAGTCA24710.11341094785115857RNA PCR Primer, Index 1 (100% over 32bp)
ACCACGTTCCCGTGGTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACGGC24150.1108407280698292RNA PCR Primer, Index 6 (97% over 36bp)
ACCGGGTGCTGTAGGCTTTTGGAATTCTCGGGTGCCAAGGAACTCCAGTCA22970.10542490781631374RNA PCR Primer, Index 1 (100% over 32bp)
CCGGGTGCTGTAGGCTTTTGGAATTCTCGGGTGCCAAGGAACTCCAGTCAC22830.10478235287098141RNA PCR Primer, Index 1 (100% over 33bp)
TTGGGCGCCATGGAATTCTCGGGTGCCAAGGAACTCCAGTCACGGCTACAT22710.10423159148926794RNA PCR Primer, Index 11 (100% over 41bp)
ACCGCTCTGACCATGGAATTCTCGGGTGCCAAGGAACTCCAGTCACGGCTA21980.10088112641717788RNA PCR Primer, Index 11 (100% over 38bp)
AGACCGCCTGGGAATACCGGGTGCTGTAGGCTTTGGAATTCTCGGGTGCCA21900.10051395216270226No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTAGGG302.158502E-645.0238841
AGTAAGG253.8797847E-545.0238841
GTTTACG351.2075543E-745.023881
AACATTC3700.045.023881
TGAGGTA2650.045.023881
TCTTTCA1250.045.023881
AAGCTGC207.0163177E-445.023881
AGCTTAT4450.045.0125122
ACGTCTA207.032206E-445.00321232
GATCACA253.894011E-544.99598711
TTCAACG2800.044.9959875
CATTCAT700.044.9959873
ATCACAC253.894011E-544.99598712
GCCGAGT351.2134478E-744.99598710
TCGTGCA253.894011E-544.99598712
TAATTCA351.2134478E-744.9959877
AGGCAAC253.894011E-544.99598711
GCTTACA253.894011E-544.9959877
TCAGAAG253.894011E-544.99598714
AGTAGTT700.044.9959877
Produced by FastQC (version 0.11.5)