FastQCFastQC Report
Wed 27 Apr 2022
EGAF00005732223

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005732223
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1865801
Sequences flagged as poor quality0
Sequence length51
%GC53

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TGGAATTCTCGGGTGCCAAGGAACTCCAGTCACTAGCTTATCTCGTATGCC28806415.439159910408451RNA PCR Primer, Index 10 (100% over 51bp)
GGAATACCGGGTGCTGTAGGCTTTTGGAATTCTCGGGTGCCAAGGAACTCC335551.798423304521758RNA PCR Primer, Index 1 (100% over 27bp)
GTTTCCGTAGTGTAGTGGTTATCACGTTCGCCTTGGAATTCTCGGGTGCCA221281.1859785689899405No Hit
TCCTTCATTCCACCGGAGTCTGTGGAATTCTCGGGTGCCAAGGAACTCCAG193581.0375168627308056RNA PCR Primer, Index 1 (100% over 29bp)
GGAATACCGGGTGCTGTAGGCTTTGGAATTCTCGGGTGCCAAGGAACTCCA192681.03269319718448RNA PCR Primer, Index 1 (100% over 28bp)
TCTGAGCGTCGCTTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACTAGCT189471.0154887900692517RNA PCR Primer, Index 10 (100% over 38bp)
GCATTGGTGGTTCAGTGGTAGAATTCTCGCCTTGGAATTCTCGGGTGCCAA188641.0110402985098625No Hit
GAATACCGGGTGCTGTAGGCTTTTGGAATTCTCGGGTGCCAAGGAACTCCA187901.0070741735051059RNA PCR Primer, Index 1 (100% over 28bp)
TCGTACGACTCTTAGCGGTGGAATTCTCGGGTGCCAAGGAACTCCAGTCAC180510.9674665197413872RNA PCR Primer, Index 1 (100% over 33bp)
CCTGTCTGAGCGTCGCTTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACT172100.922392045025166RNA PCR Primer, Index 3 (100% over 34bp)
CGCCTGTCTGAGCGTCGCTTGGAATTCTCGGGTGCCAAGGAACTCCAGTCA139420.7472393894096959RNA PCR Primer, Index 1 (100% over 32bp)
GAATACCGGGTGCTGTAGGCTTTGGAATTCTCGGGTGCCAAGGAACTCCAG138760.7437020346757237RNA PCR Primer, Index 1 (100% over 29bp)
AATACCGGGTGCTGTAGGCTTTGGAATTCTCGGGTGCCAAGGAACTCCAGT135260.7249433353289017RNA PCR Primer, Index 1 (100% over 30bp)
AATACCGGGTGCTGTAGGCTTTTGGAATTCTCGGGTGCCAAGGAACTCCAG115520.6191442710128251RNA PCR Primer, Index 1 (100% over 29bp)
ATACCGGGTGCTGTAGGCTTTGGAATTCTCGGGTGCCAAGGAACTCCAGTC111930.5999032051113704RNA PCR Primer, Index 1 (100% over 31bp)
ATACCGGGTGCTGTAGGCTTTTGGAATTCTCGGGTGCCAAGGAACTCCAGT105770.5668878942609635RNA PCR Primer, Index 1 (100% over 30bp)
TACCGGGTGCTGTAGGCTTTGGAATTCTCGGGTGCCAAGGAACTCCAGTCA100060.5362844161837195RNA PCR Primer, Index 1 (100% over 32bp)
TACCCTGTAGAACCGAATTTGTTGGAATTCTCGGGTGCCAAGGAACTCCAG99780.5347837202359736RNA PCR Primer, Index 1 (100% over 29bp)
TGTCTGAGCGTCGCTTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACTAG99740.5345693351005815RNA PCR Primer, Index 7 (97% over 36bp)
GTCTGAGCGTCGCTTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACTAGC99140.5313535580696976RNA PCR Primer, Index 10 (100% over 37bp)
GGGAATACCGGGTGCTGTAGGCTTTTGGAATTCTCGGGTGCCAAGGAACTC96380.5165609837276323RNA PCR Primer, Index 1 (100% over 26bp)
TGGGAATACCGGGTGCTGTAGGCTTTTGGAATTCTCGGGTGCCAAGGAACT92200.4941577370791419RNA PCR Primer, Index 1 (100% over 25bp)
GCCTGTCTGAGCGTCGCTTGGAATTCTCGGGTGCCAAGGAACTCCAGTCAC89220.4781860444924191RNA PCR Primer, Index 1 (100% over 33bp)
TACCGGGTGCTGTAGGCTTTTGGAATTCTCGGGTGCCAAGGAACTCCAGTC88870.47631017455773683RNA PCR Primer, Index 1 (100% over 31bp)
CTACGCCTGTCTGAGCGTCGCTTGGAATTCTCGGGTGCCAAGGAACTCCAG85590.45873059345557216RNA PCR Primer, Index 1 (100% over 29bp)
CCGGGTGCTGTAGGCTTTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACT77470.4152104109709449RNA PCR Primer, Index 3 (100% over 34bp)
CTCGACACAAGGGTTTGTTGGAATTCTCGGGTGCCAAGGAACTCCAGTCAC75530.40481273190442074RNA PCR Primer, Index 1 (100% over 33bp)
CTGGGAATACCGGGTGCTGTAGGCTTTTGGAATTCTCGGGTGCCAAGGAAC74490.3992387183842221RNA PCR Primer, Index 1 (100% over 24bp)
GCATGGGTGGTTCAGTGGTAGAATTCTCGCCTTGGAATTCTCGGGTGCCAA72650.3893770021561785No Hit
TAGCTTATCAGACTGATGTTGACTGGAATTCTCGGGTGCCAAGGAACTCCA69460.3722797876086464RNA PCR Primer, Index 1 (100% over 28bp)
ACCGGGTGCTGTAGGCTTTGGAATTCTCGGGTGCCAAGGAACTCCAGTCAC69340.3716366322024696RNA PCR Primer, Index 1 (100% over 33bp)
CTGTCTGAGCGTCGCTTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACTA66080.35416424366800103RNA PCR Primer, Index 7 (97% over 35bp)
CGACACAAGGGTTTGTTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACTA64770.34714313048390477RNA PCR Primer, Index 7 (97% over 35bp)
GGGAATACCGGGTGCTGTAGGCTTTGGAATTCTCGGGTGCCAAGGAACTCC64720.34687514906466443RNA PCR Primer, Index 1 (100% over 27bp)
ACCGGGTGCTGTAGGCTTTTGGAATTCTCGGGTGCCAAGGAACTCCAGTCA61220.3281164497178424RNA PCR Primer, Index 1 (100% over 32bp)
CCGGGTGCTGTAGGCTTTTGGAATTCTCGGGTGCCAAGGAACTCCAGTCAC60910.3264549649185524RNA PCR Primer, Index 1 (100% over 33bp)
CAAGGGTTTGTTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACTAGCTTA60510.3243111135646299RNA PCR Primer, Index 10 (100% over 40bp)
TGGGAATACCGGGTGCTGTAGGCTTTGGAATTCTCGGGTGCCAAGGAACTC57550.3084466135456032RNA PCR Primer, Index 1 (100% over 26bp)
TAGCTTATCAGACTGATGTTGACTTGGAATTCTCGGGTGCCAAGGAACTCC53930.2890447587926044RNA PCR Primer, Index 1 (100% over 27bp)
TGAGATGAAGCACTGTAGCTTGGAATTCTCGGGTGCCAAGGAACTCCAGTC52820.28309557128546936RNA PCR Primer, Index 1 (100% over 31bp)
CCCCACGTTGGGCGCCATGGAATTCTCGGGTGCCAAGGAACTCCAGTCACT52560.2817020679054197RNA PCR Primer, Index 3 (100% over 34bp)
TCGACACAAGGGTTTGTTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACT52060.2790222537130166RNA PCR Primer, Index 3 (100% over 34bp)
CTGAGCGTCGCTTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACTAGCTT50980.27323385505742576RNA PCR Primer, Index 10 (100% over 39bp)
GCATTGTGGTTCAGTGGTAGAATTCTCGCCTTGGAATTCTCGGGTGCCAAG50870.27264429593509704No Hit
AATACCGGGTGCTGTAGGCTTGGAATTCTCGGGTGCCAAGGAACTCCAGTC50020.2680886118080117RNA PCR Primer, Index 1 (100% over 31bp)
TCGTGGTTGTAGTCCGTGCGAGAATACCATGGAATTCTCGGGTGCCAAGGA49910.26749905268568297RNA PCR Primer, Index 1 (100% over 22bp)
CCTGGGAATACCGGGTGCTGTAGGCTTTTGGAATTCTCGGGTGCCAAGGAA49610.26589116417024106RNA PCR Primer, Index 1 (100% over 23bp)
CGGGTGCTGTAGGCTTTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACTA48510.25999557294695413RNA PCR Primer, Index 7 (97% over 35bp)
ACACAAGGGTTTGTTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACTAGC48270.25870926213460066RNA PCR Primer, Index 10 (100% over 37bp)
CACAAGGGTTTGTTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACTAGCT47890.2566726033483742RNA PCR Primer, Index 10 (100% over 38bp)
GCATTGGTGGTTCAGTGGTAGAATTCTCGCCTGCTGGAATTCTCGGGTGCC46990.2518489378020486No Hit
GTACGACTCTTAGCGGTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACTA46910.25142016753126406RNA PCR Primer, Index 7 (97% over 35bp)
GGGGCTACGCCTGTCTGAGCGTCGCTTGGAATTCTCGGGTGCCAAGGAACT46490.24916912360964538RNA PCR Primer, Index 1 (100% over 25bp)
GAATACCGGGTGCTGTAGGCTTGGAATTCTCGGGTGCCAAGGAACTCCAGT44400.2379675002854002RNA PCR Primer, Index 1 (100% over 30bp)
TAGCTTATCAGACTGATGTTGATGGAATTCTCGGGTGCCAAGGAACTCCAG43440.23282225703598614RNA PCR Primer, Index 1 (100% over 29bp)
AAGGGTTTGTTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACTAGCTTAT42700.22885613203122945RNA PCR Primer, Index 10 (100% over 41bp)
ATACCGGGTGCTGTAGGCTTGGAATTCTCGGGTGCCAAGGAACTCCAGTCA42610.2283737654765969RNA PCR Primer, Index 1 (100% over 32bp)
AGGGTTTGTTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACTAGCTTATC42550.22805218777350852RNA PCR Primer, Index 10 (100% over 42bp)
CTGGGAATACCGGGTGCTGTAGGCTTTGGAATTCTCGGGTGCCAAGGAACT40350.21626100532693465RNA PCR Primer, Index 1 (100% over 25bp)
GGAATACCGGGTGCTGTAGGCTTGGAATTCTCGGGTGCCAAGGAACTCCAG39510.21175891748369735RNA PCR Primer, Index 1 (100% over 29bp)
TCCTTCATTCCACCGGAGTCTGTTGGAATTCTCGGGTGCCAAGGAACTCCA39510.21175891748369735RNA PCR Primer, Index 1 (100% over 28bp)
TGAGCGTCGCTTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACTAGCTTA38470.20618490396349878RNA PCR Primer, Index 10 (100% over 40bp)
GGTGCTGTAGGCTTTTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACTAG38430.20597051882810655RNA PCR Primer, Index 7 (97% over 36bp)
CCTGGGAATACCGGGTGCTGTAGGCTTTGGAATTCTCGGGTGCCAAGGAAC37280.19980694618557926RNA PCR Primer, Index 1 (100% over 24bp)
CGGGTGCTGTAGGCTTTTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACT36790.1971807282770242RNA PCR Primer, Index 3 (100% over 34bp)
GGAATTCTCGGGTGCCAAGGAACTCCAGTCACTAGCTTATCTCGTATGCCG35590.1907491742152566RNA PCR Primer, Index 10 (100% over 51bp)
TACCGGGTGCTGTAGGCTTGGAATTCTCGGGTGCCAAGGAACTCCAGTCAC35570.1906419816475605RNA PCR Primer, Index 1 (100% over 33bp)
TTCACAGTGGCTAAGTTCTGTGGAATTCTCGGGTGCCAAGGAACTCCAGTC35020.18769418603591703RNA PCR Primer, Index 1 (100% over 31bp)
GCATTGGTGGTTCAGTGGTAGAATTCTCGCCTGGAATTCTCGGGTGCCAAG34580.18533594954660224No Hit
GGTGCTGTAGGCTTTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACTAGC33710.18067307285182074RNA PCR Primer, Index 10 (100% over 37bp)
CCACGTTCCCGTGGTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACTAGC33400.17901158805253078RNA PCR Primer, Index 10 (100% over 37bp)
GTAGTCGTGGCCTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACTAGCTT32830.17595659987319118RNA PCR Primer, Index 10 (100% over 39bp)
TACGCCTGTCTGAGCGTCGCTTGGAATTCTCGGGTGCCAAGGAACTCCAGT31020.16625567249669176RNA PCR Primer, Index 1 (100% over 30bp)
GTGCTGTAGGCTTTTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACTAGC30040.16100323667958158RNA PCR Primer, Index 10 (100% over 37bp)
TCCCTGTTCGGGCGCCATGGAATTCTCGGGTGCCAAGGAACTCCAGTCACT29130.15612597484940785RNA PCR Primer, Index 3 (100% over 34bp)
GGGCTACGCCTGTCTGAGCGTCGCTTGGAATTCTCGGGTGCCAAGGAACTC28060.1503911724776651RNA PCR Primer, Index 1 (100% over 26bp)
CCGCCTGGGAATACCGGGTGCTGTAGGCTTTGGAATTCTCGGGTGCCAAGG27100.14524592922825103Illumina Small RNA Adapter 2 (100% over 21bp)
CCCACGTTGGGCGCCATGGAATTCTCGGGTGCCAAGGAACTCCAGTCACTA26800.14363804071280914RNA PCR Primer, Index 7 (97% over 35bp)
CGGGGCTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACTAGCTTATCTCG26660.14288769273893626RNA PCR Primer, Index 10 (100% over 45bp)
CCACGTTGGGCGCCATGGAATTCTCGGGTGCCAAGGAACTCCAGTCACTAG26630.14272690388739206RNA PCR Primer, Index 7 (97% over 36bp)
CCGGGTGCTGTAGGCTTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACTA26370.14133340050734242RNA PCR Primer, Index 7 (97% over 35bp)
CGGGGCTACGCCTGTCTGAGCGTCGCTTGGAATTCTCGGGTGCCAAGGAAC26120.13999349341114084RNA PCR Primer, Index 1 (100% over 24bp)
ACCGGGTGCTGTAGGCTTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACT24630.13200764711777943RNA PCR Primer, Index 3 (100% over 34bp)
CAACACCAGTCGATGGGCTGTTGGAATTCTCGGGTGCCAAGGAACTCCAGT24630.13200764711777943RNA PCR Primer, Index 1 (100% over 30bp)
GTCTACGGCCTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACTAGCTTAT24270.13007818089924916RNA PCR Primer, Index 10 (100% over 41bp)
CTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACTAGCTTATCTCGTATGC24160.12948862177692047RNA PCR Primer, Index 10 (100% over 50bp)
TGAGGTAGTAGTTTGTGCTGTTTGGAATTCTCGGGTGCCAAGGAACTCCAG24100.12916704407383212RNA PCR Primer, Index 1 (100% over 29bp)
GTGCTGTAGGCTTTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACTAGCT23660.12680880758451732RNA PCR Primer, Index 10 (100% over 38bp)
ATACCGGGTGCTGTAGGCTTATGGAATTCTCGGGTGCCAAGGAACTCCAGT23650.12675521130066925RNA PCR Primer, Index 1 (100% over 30bp)
CCTCGACACAAGGGTTTGTTGGAATTCTCGGGTGCCAAGGAACTCCAGTCA21840.11705428392416983RNA PCR Primer, Index 1 (100% over 32bp)
AAGCTGCCAGTTGAAGAACTGTTGGAATTCTCGGGTGCCAAGGAACTCCAG20930.1121770220939961RNA PCR Primer, Index 1 (100% over 29bp)
CACGTTGGGCGCCATGGAATTCTCGGGTGCCAAGGAACTCCAGTCACTAGC20810.11153386668781932RNA PCR Primer, Index 10 (100% over 37bp)
ACGCCTGTCTGAGCGTCGCTTGGAATTCTCGGGTGCCAAGGAACTCCAGTC20220.10837168594078361RNA PCR Primer, Index 1 (100% over 31bp)
CCGGGGCTACGCCTGTCTGAGCGTCGCTTGGAATTCTCGGGTGCCAAGGAA19700.10558467918068432RNA PCR Primer, Index 1 (100% over 23bp)
CGTTCGCGCTTTCCCCTGTGGAATTCTCGGGTGCCAAGGAACTCCAGTCAC18990.10177934302747185RNA PCR Primer, Index 1 (100% over 33bp)
ACAAGGGTTTGTTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACTAGCTT18820.10086820620205476RNA PCR Primer, Index 10 (100% over 39bp)

[FAIL]Adapter Content

Adapter graph