FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005732225

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005732225
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1770796
Sequences flagged as poor quality0
Sequence length51
%GC55

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TCGTACGACTCTTAGCGGTGGAATTCTCGGGTGCCAAGGAACTCCAGTCAC21804412.313332535198859RNA PCR Primer, Index 1 (100% over 33bp)
TGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCAGGCGATCTCGTATGCC384262.169984571910034RNA PCR Primer, Index 33 (100% over 51bp)
GTACGACTCTTAGCGGTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCA374602.115432833595739RNA PCR Primer, Index 7 (100% over 35bp)
GGAATACCGGGTGCTGTAGGCTTTTGGAATTCTCGGGTGCCAAGGAACTCC235341.3290068421207186RNA PCR Primer, Index 1 (100% over 27bp)
GGAATACCGGGTGCTGTAGGCTTTGGAATTCTCGGGTGCCAAGGAACTCCA204781.1564290861284983RNA PCR Primer, Index 1 (100% over 28bp)
TTGTACGACTCTTAGCGGTGGAATTCTCGGGTGCCAAGGAACTCCAGTCAC183671.0372171610959138RNA PCR Primer, Index 1 (100% over 33bp)
TAGCTTATCAGACTGATGTTGACTGGAATTCTCGGGTGCCAAGGAACTCCA163990.9260807004307666RNA PCR Primer, Index 1 (100% over 28bp)
TCCTTCATTCCACCGGAGTCTGTGGAATTCTCGGGTGCCAAGGAACTCCAG162130.9155769495752192RNA PCR Primer, Index 1 (100% over 29bp)
GCATTGGTGGTTCAGTGGTAGAATTCTCGCCTGCTGGAATTCTCGGGTGCC154500.8724889823559574No Hit
GAATACCGGGTGCTGTAGGCTTTTGGAATTCTCGGGTGCCAAGGAACTCCA137290.7753010510527468RNA PCR Primer, Index 1 (100% over 28bp)
GAATACCGGGTGCTGTAGGCTTTGGAATTCTCGGGTGCCAAGGAACTCCAG132350.7474039923288736RNA PCR Primer, Index 1 (100% over 29bp)
TAGCTTATCAGACTGATGTTGATGGAATTCTCGGGTGCCAAGGAACTCCAG131420.7421521169010998RNA PCR Primer, Index 1 (100% over 29bp)
TTCACAGTGGCTAAGTTCTGTGGAATTCTCGGGTGCCAAGGAACTCCAGTC114230.6450771291554759RNA PCR Primer, Index 1 (100% over 31bp)
AATACCGGGTGCTGTAGGCTTTGGAATTCTCGGGTGCCAAGGAACTCCAGT97140.5485668591977845RNA PCR Primer, Index 1 (100% over 30bp)
TACCCTGTAGAACCGAATTTGTTGGAATTCTCGGGTGCCAAGGAACTCCAG87970.4967822380443597RNA PCR Primer, Index 1 (100% over 29bp)
TCGTATGACTCTTAGCGGTGGAATTCTCGGGTGCCAAGGAACTCCAGTCAC85050.48029247863672603RNA PCR Primer, Index 1 (100% over 33bp)
TGAGATGAAGCACTGTAGCTTGGAATTCTCGGGTGCCAAGGAACTCCAGTC84640.4779771357062022RNA PCR Primer, Index 1 (100% over 31bp)
AATACCGGGTGCTGTAGGCTTTTGGAATTCTCGGGTGCCAAGGAACTCCAG70840.4000460809714953RNA PCR Primer, Index 1 (100% over 29bp)
ACACAAGGGTTTGTTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCAGG67950.3837257369002415RNA PCR Primer, Index 31 (97% over 37bp)
CCACGTTCCCGTGGTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCAGG66880.377683256569362RNA PCR Primer, Index 31 (97% over 37bp)
AATTCTCGGGTGCCAAGGAACTCCAGTCACCAGGCGATCTCGTATGCCGTC63740.35995111802827656RNA PCR Primer, Index 33 (100% over 51bp)
CGCGACCTCAGATCAGACGTGGCGACCCGCTGATGGAATTCTCGGGTGCCA57230.3231879900338605No Hit
TGAGGTAGTAGGTTGTATAGTTTGGAATTCTCGGGTGCCAAGGAACTCCAG53400.30155929875603965RNA PCR Primer, Index 1 (100% over 29bp)
CCTGTCTGAGCGTCGCTTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACC50470.28501306756961275RNA PCR Primer, Index 2 (100% over 34bp)
AAGCTGCCAGTTGAAGAACTGTTGGAATTCTCGGGTGCCAAGGAACTCCAG48200.27219397378354143RNA PCR Primer, Index 1 (100% over 29bp)
TGAGGTAGTAGATTGTATAGTTTGGAATTCTCGGGTGCCAAGGAACTCCAG45680.2579630855276384RNA PCR Primer, Index 1 (100% over 29bp)
TTCACAGTGGCTAAGTTCTGCTGGAATTCTCGGGTGCCAAGGAACTCCAGT44090.24898407269950915RNA PCR Primer, Index 1 (100% over 30bp)
TTTCGGCACCATGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCAGGCGA43880.24779816534485055RNA PCR Primer, Index 33 (100% over 40bp)
TATTGCACTTGTCCCGGCCTGTTGGAATTCTCGGGTGCCAAGGAACTCCAG43500.24565223775070646RNA PCR Primer, Index 1 (100% over 29bp)
GTTTGTGATGACTTACATGGAATTCTCGGGTGCCAAGGAACTCCAGTCACC43010.24288512058983644RNA PCR Primer, Index 2 (100% over 34bp)
TCCTTCATTCCACCGGAGTCTGTTGGAATTCTCGGGTGCCAAGGAACTCCA41660.23526143045274556RNA PCR Primer, Index 1 (100% over 28bp)
GGAATTCTCGGGTGCCAAGGAACTCCAGTCACCAGGCGATCTCGTATGCCG40390.2280895145460008RNA PCR Primer, Index 33 (100% over 51bp)
CAAGGGTTTGTTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCAGGCGA40250.22729890964289506RNA PCR Primer, Index 33 (100% over 40bp)
GGAATACCGGGTGCTGTAGGCTTGGAATTCTCGGGTGCCAAGGAACTCCAG38750.21882814282390517RNA PCR Primer, Index 1 (100% over 29bp)
GTCTACGGCCATACCACCCTGAACGCGCCCGATGGAATTCTCGGGTGCCAA38740.21877167104511192No Hit
AACATTCAACGCTGTCGGTGAGTGGAATTCTCGGGTGCCAAGGAACTCCAG37660.2126727189354392RNA PCR Primer, Index 1 (100% over 29bp)
TGAGGTAGTAGTTTGTGCTGTTTGGAATTCTCGGGTGCCAAGGAACTCCAG37440.21143033980198736RNA PCR Primer, Index 1 (100% over 29bp)
GCATTGTGGTTCAGTGGTAGAATTCTCGCCTGCTGGAATTCTCGGGTGCCA37060.20928441220784325No Hit
ATACCGGGTGCTGTAGGCTTTGGAATTCTCGGGTGCCAAGGAACTCCAGTC34390.19420644727004127RNA PCR Primer, Index 1 (100% over 31bp)
GAGACCGCCTGGGAATACCGGGTGCTGTAGGCTTTGGAATTCTCGGGTGCC33110.18697805958450323No Hit
TCGTGGTTGTAGTCCGTGCGAGAATACCATGGAATTCTCGGGTGCCAAGGA32940.18601803934501773RNA PCR Primer, Index 1 (100% over 22bp)
CATTGCACTTGTCTCGGTCTGATGGAATTCTCGGGTGCCAAGGAACTCCAG32610.18415447064483995RNA PCR Primer, Index 1 (100% over 29bp)
CCTGGGAATACCGGGTGCTGTAGGCTTTGGAATTCTCGGGTGCCAAGGAAC32530.18370269641449383RNA PCR Primer, Index 1 (100% over 24bp)
GGTGCTGTAGGCTTTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCAGG32240.1820650148294891RNA PCR Primer, Index 31 (97% over 37bp)
GAATACCGGGTGCTGTAGGCTTGGAATTCTCGGGTGCCAAGGAACTCCAGT31340.17698255473809518RNA PCR Primer, Index 1 (100% over 30bp)
CCTGGGAATACCGGGTGCTGTAGGCTTTTGGAATTCTCGGGTGCCAAGGAA30590.17274717132860024RNA PCR Primer, Index 1 (100% over 23bp)
TCCAGGGTTCAGGTCCCTGTTCGGGCGCCATGGAATTCTCGGGTGCCAAGG30180.17043182839807633Illumina Small RNA Adapter 2 (100% over 21bp)
CGACACAAGGGTTTGTTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCA29830.16845531614031203RNA PCR Primer, Index 7 (100% over 35bp)
TCCAGGGTTCAAGTCCCTGTTCGGGCGCCATGGAATTCTCGGGTGCCAAGG29670.1675517676796198Illumina Small RNA Adapter 2 (100% over 21bp)
CACCCGTAGAACCGACCTTGCGTGGAATTCTCGGGTGCCAAGGAACTCCAG29460.1663658603249612RNA PCR Primer, Index 1 (100% over 29bp)
TCTGAGCGTCGCTTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCAGGC29210.16495406585512956RNA PCR Primer, Index 33 (100% over 38bp)
TCGTGGGTTCGAGCCCCACGTTGGGCGCCATGGAATTCTCGGGTGCCAAGG29180.16478465051874977Illumina Small RNA Adapter 2 (100% over 21bp)
AACATTCAACGCTGTCGGTGAGTTGGAATTCTCGGGTGCCAAGGAACTCCA28980.16365521494288443RNA PCR Primer, Index 1 (100% over 28bp)
TTCAAGTAATCCAGGATAGGCTTGGAATTCTCGGGTGCCAAGGAACTCCAG28490.1608880977820144RNA PCR Primer, Index 1 (100% over 29bp)
GCATTGGTGGTTCAGTGGTAGAATTCTCGCCTTGGAATTCTCGGGTGCCAA28420.16049279533046154No Hit
GGTGCTGTAGGCTTTTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCAG27800.15699154504527907RNA PCR Primer, Index 7 (100% over 36bp)
TAGCTTATCAGACTGATGTTGACTTGGAATTCTCGGGTGCCAAGGAACTCC27590.1558056376906205RNA PCR Primer, Index 1 (100% over 27bp)
TAGCTTATCAGACTGATGTTGTGGAATTCTCGGGTGCCAAGGAACTCCAGT27390.15467620211475516RNA PCR Primer, Index 1 (100% over 30bp)
ATACCGGGTGCTGTAGGCTTTTGGAATTCTCGGGTGCCAAGGAACTCCAGT27300.15416795610561576RNA PCR Primer, Index 1 (100% over 30bp)
GTCTGAGCGTCGCTTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCAGG27080.15292557697216394RNA PCR Primer, Index 31 (97% over 37bp)
GCCCGGCTAGCTCAGTCGGTAGAGCATGGGACTCTGGAATTCTCGGGTGCC27020.15258674629940433No Hit
GCCCGGCTAGCTCAGTCGGTAGAGCATGAGACTCTGGAATTCTCGGGTGCC26670.15061023404164003No Hit
TTCGGGCGCCATGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCAGGCGA25980.1467136813049047RNA PCR Primer, Index 33 (100% over 40bp)
TCGACACAAGGGTTTGTTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACC25800.1456971892866259RNA PCR Primer, Index 2 (100% over 34bp)
CTCGGGTGCCAAGGAACTCCAGTCACCAGGCGATCTCGTATGCCGTCTTCT25640.14479364082593366RNA PCR Primer, Index 33 (100% over 51bp)
CGTACGACTCTTAGCGGTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACC25500.14400303592282793RNA PCR Primer, Index 2 (100% over 34bp)
CAACGGAATCCCAAAAGCAGCTGTGGAATTCTCGGGTGCCAAGGAACTCCA25450.1437206770288616RNA PCR Primer, Index 1 (100% over 28bp)
CTCGTACGACTCTTAGCGGTGGAATTCTCGGGTGCCAAGGAACTCCAGTCA25140.14197005188627035RNA PCR Primer, Index 1 (100% over 32bp)
GTTTCGGCACCATGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCAGGCG25020.14129239054075118RNA PCR Primer, Index 33 (100% over 39bp)
AATACCGGGTGCTGTAGGCTTATGGAATTCTCGGGTGCCAAGGAACTCCAG24350.13750878136160236RNA PCR Primer, Index 1 (100% over 29bp)
TAGCAGCACGTAAATATTGGCGTGGAATTCTCGGGTGCCAAGGAACTCCAG24190.1366052329009101RNA PCR Primer, Index 1 (100% over 29bp)
GTCTACGGCCATACCACCCTGAACGCGCCCGATCTGGAATTCTCGGGTGCC23980.13541932554625152No Hit
GTCTACGGCCATACCACCCTGAACGCGCCCGATTGGAATTCTCGGGTGCCA23780.13428988997038618No Hit
AATACCGGGTGCTGTAGGCTTGGAATTCTCGGGTGCCAAGGAACTCCAGTC23600.1332733979521074RNA PCR Primer, Index 1 (100% over 31bp)
ACCACGTTCCCGTGGTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCAG23360.13191807526106905RNA PCR Primer, Index 7 (100% over 36bp)
TGTCTGAGCGTCGCTTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCAG22990.1298286194457182RNA PCR Primer, Index 7 (100% over 36bp)
GTCTACGGCCATACCACCCTGAACGCGCCCGTGGAATTCTCGGGTGCCAAG22990.1298286194457182No Hit
CCGGGTGCTGTAGGCTTTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACC22710.12824740963950676RNA PCR Primer, Index 2 (100% over 34bp)
CTCGACACAAGGGTTTGTTGGAATTCTCGGGTGCCAAGGAACTCCAGTCAC22650.12790857896674715RNA PCR Primer, Index 1 (100% over 33bp)
ATGGGAGACCGCCTGGGAATACCGGGTGCTGTAGGCTTTGGAATTCTCGGG22360.12627089738174244No Hit
CATTATTACTTTTGGTACGCGTGGAATTCTCGGGTGCCAAGGAACTCCAGT22310.12598853848777614RNA PCR Primer, Index 1 (100% over 30bp)
AGACCGCCTGGGAATACCGGGTGCTGTAGGCTTTGGAATTCTCGGGTGCCA22270.12576265137260306No Hit
GGGAATACCGGGTGCTGTAGGCTTTGGAATTCTCGGGTGCCAAGGAACTCC22170.1251979335846704RNA PCR Primer, Index 1 (100% over 27bp)
ACCGGGTGCTGTAGGCTTTGGAATTCTCGGGTGCCAAGGAACTCCAGTCAC22110.1248591029119108RNA PCR Primer, Index 1 (100% over 33bp)
TACTTGGATGGGAGACCGCCTGGAATTCTCGGGTGCCAAGGAACTCCAGTC21940.12389908267242528RNA PCR Primer, Index 1 (100% over 31bp)
AGGGTTTGTTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCAGGCGATC21710.12260023176018017RNA PCR Primer, Index 33 (100% over 42bp)
GTAGTCGTGGCCTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCAGGCG21670.12237434464500711RNA PCR Primer, Index 33 (100% over 39bp)
GAATACCGGGTGCTGTAGGCTTATGGAATTCTCGGGTGCCAAGGAACTCCA21630.12214845752983403RNA PCR Primer, Index 1 (100% over 28bp)
GTTTCCGTAGTGTAGTGGTTATCACGTTCGCCTCTGGAATTCTCGGGTGCC20930.11819543301430542No Hit
CGCGACCTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCAGGCGATCTC20760.11723541277481991RNA PCR Primer, Index 33 (100% over 44bp)
GGGAATACCGGGTGCTGTAGGCTTTTGGAATTCTCGGGTGCCAAGGAACTC20620.1164448078717142RNA PCR Primer, Index 1 (100% over 26bp)
GGAATACCGGGTGCTGTAGGCTTATGGAATTCTCGGGTGCCAAGGAACTCC20270.11446829561394989RNA PCR Primer, Index 1 (100% over 27bp)
CACCACGTTCCCGTGGTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCA20190.11401652138360374RNA PCR Primer, Index 7 (100% over 35bp)
TACCGGGTGCTGTAGGCTTTGGAATTCTCGGGTGCCAAGGAACTCCAGTCA19720.11136234778032025RNA PCR Primer, Index 1 (100% over 32bp)
TGAGATGAAGCACTGTAGCTCTTGGAATTCTCGGGTGCCAAGGAACTCCAG19540.11034585576204148RNA PCR Primer, Index 1 (100% over 29bp)
CCCCACGTTGGGCGCCATGGAATTCTCGGGTGCCAAGGAACTCCAGTCACC19530.11028938398324822RNA PCR Primer, Index 2 (100% over 34bp)
CAACACCAGTCGATGGGCTGTTGGAATTCTCGGGTGCCAAGGAACTCCAGT19340.10921642018617615RNA PCR Primer, Index 1 (100% over 30bp)
CACAAGGGTTTGTTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCAGGC19130.10803051283151757RNA PCR Primer, Index 33 (100% over 38bp)
CTGGGAATACCGGGTGCTGTAGGCTTTTGGAATTCTCGGGTGCCAAGGAAC18830.1063363594677196RNA PCR Primer, Index 1 (100% over 24bp)
TGAGATGAAGCACTGTAGCTCTGGAATTCTCGGGTGCCAAGGAACTCCAGT18680.10548928278582062RNA PCR Primer, Index 1 (100% over 30bp)
ACACAAGGGTTTGTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCAGGC18460.10424690365236876RNA PCR Primer, Index 33 (100% over 38bp)
CGGGTGCTGTAGGCTTTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCA18390.1038516012008159RNA PCR Primer, Index 7 (100% over 35bp)
CTCGTCGCCATGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCAGGCGAT18040.10187508894305158RNA PCR Primer, Index 33 (100% over 41bp)
GTCTACGGCCTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCAGGCGAT17980.10153625827029202RNA PCR Primer, Index 33 (100% over 41bp)

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAGTTTA700.045.017661
TTAATGC406.7957444E-945.0176541
GTTTACG253.8826587E-545.0176541
TCTTACG406.7957444E-945.0176541
CGGGACA207.020741E-445.0176541
GAAAAAG253.8826587E-545.0176541
AGCTACA1500.045.0176541
TCCCATA207.020741E-445.0176541
AGTTTAT700.045.010032
GGCAAGA1150.045.010032
GCTACAT1500.045.010032
GTAAGGT1450.045.010032
GTTACAT207.026605E-445.0100252
TAGGGCG207.026605E-445.0100252
ACGCTCA253.8865466E-545.0100252
CGTAAGA406.8048394E-945.0100252
TATAATA253.8865466E-545.0100252
TAATGCT406.8048394E-945.0100252
TAGCATA351.2108467E-745.0074921
AATGCGC351.2108467E-745.0074922