FastQCFastQC Report
Wed 27 Apr 2022
EGAF00005732229

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005732229
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences885530
Sequences flagged as poor quality0
Sequence length51
%GC55

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TCGTACGACTCTTAGCGGTGGAATTCTCGGGTGCCAAGGAACTCCAGTCAC426924.821067609228372RNA PCR Primer, Index 1 (100% over 33bp)
GTACGACTCTTAGCGGTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCG176021.9877361580070692RNA PCR Primer, Index 2 (100% over 35bp)
GGAATACCGGGTGCTGTAGGCTTTTGGAATTCTCGGGTGCCAAGGAACTCC148781.6801237676871479RNA PCR Primer, Index 1 (100% over 27bp)
GGAATACCGGGTGCTGTAGGCTTTGGAATTCTCGGGTGCCAAGGAACTCCA124871.4101159757433401RNA PCR Primer, Index 1 (100% over 28bp)
GAATACCGGGTGCTGTAGGCTTTTGGAATTCTCGGGTGCCAAGGAACTCCA92041.0393775479091618RNA PCR Primer, Index 1 (100% over 28bp)
GAATACCGGGTGCTGTAGGCTTTGGAATTCTCGGGTGCCAAGGAACTCCAG90881.0262780481745397RNA PCR Primer, Index 1 (100% over 29bp)
ACACAAGGGTTTGTTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCGTA76390.8626472282136122RNA PCR Primer, Index 22 (100% over 37bp)
AATACCGGGTGCTGTAGGCTTTGGAATTCTCGGGTGCCAAGGAACTCCAGT71730.8100233758314229RNA PCR Primer, Index 1 (100% over 30bp)
TTTCGGCACCATGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCGTACGA65500.7396700281187538RNA PCR Primer, Index 22 (100% over 40bp)
GCATTGGTGGTTCAGTGGTAGAATTCTCGCCTGCTGGAATTCTCGGGTGCC60660.685013494743261No Hit
TGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCGTACGATCTCGTATGCC56100.6335189095795738RNA PCR Primer, Index 22 (100% over 51bp)
AATACCGGGTGCTGTAGGCTTTTGGAATTCTCGGGTGCCAAGGAACTCCAG54790.6187255090171987RNA PCR Primer, Index 1 (100% over 29bp)
TTGTACGACTCTTAGCGGTGGAATTCTCGGGTGCCAAGGAACTCCAGTCAC43080.48648831773062456RNA PCR Primer, Index 1 (100% over 33bp)
TACCCTGTAGAACCGAATTTGTTGGAATTCTCGGGTGCCAAGGAACTCCAG42790.48321344279696904RNA PCR Primer, Index 1 (100% over 29bp)
GTTTCGGCACCATGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCGTACG36300.4099240003161948RNA PCR Primer, Index 22 (100% over 39bp)
TCTGAGCGTCGCTTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCGTAC35920.40563278488588755RNA PCR Primer, Index 22 (100% over 38bp)
TAGCTTATCAGACTGATGTTGATGGAATTCTCGGGTGCCAAGGAACTCCAG31710.358090634986957RNA PCR Primer, Index 1 (100% over 29bp)
TAGCTTATCAGACTGATGTTGACTGGAATTCTCGGGTGCCAAGGAACTCCA30250.34160333359682904RNA PCR Primer, Index 1 (100% over 28bp)
ACACAAGGGTTTGTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCGTAC29450.33256919584881367RNA PCR Primer, Index 22 (100% over 38bp)
CCTGTCTGAGCGTCGCTTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACC29430.3323433424051133RNA PCR Primer, Index 2 (100% over 34bp)
TCCTTCATTCCACCGGAGTCTGTGGAATTCTCGGGTGCCAAGGAACTCCAG27560.3112260454191275RNA PCR Primer, Index 1 (100% over 29bp)
AATACCGGGTGCTGTAGGCTTATGGAATTCTCGGGTGCCAAGGAACTCCAG27280.3080640972073222RNA PCR Primer, Index 1 (100% over 29bp)
GAGACCGCCTGGGAATACCGGGTGCTGTAGGCTTTGGAATTCTCGGGTGCC26870.30343410161146434No Hit
CGACACAAGGGTTTGTTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCG25300.28570460628098426RNA PCR Primer, Index 2 (100% over 35bp)
AATTCTCGGGTGCCAAGGAACTCCAGTCACCGTACGATCTCGTATGCCGTC24730.27926778313552336RNA PCR Primer, Index 22 (100% over 51bp)
TTCACAGTGGCTAAGTTCTGTGGAATTCTCGGGTGCCAAGGAACTCCAGTC24480.2764446150892686RNA PCR Primer, Index 1 (100% over 31bp)
GCATTGGTGGTTCAGTGGTAGAATTCTCGCCTGTGGAATTCTCGGGTGCCA24390.2754282745926168No Hit
GGAATACCGGGTGCTGTAGGCTTGGAATTCTCGGGTGCCAAGGAACTCCAG23180.2617641412487437RNA PCR Primer, Index 1 (100% over 29bp)
TTCGGGCGCCATGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCGTACGA22900.2586021930369384RNA PCR Primer, Index 22 (100% over 40bp)
CCTGGGAATACCGGGTGCTGTAGGCTTTGGAATTCTCGGGTGCCAAGGAAC22700.2563436585999345RNA PCR Primer, Index 1 (100% over 24bp)
CCTGGGAATACCGGGTGCTGTAGGCTTTTGGAATTCTCGGGTGCCAAGGAA21900.24730952085191918RNA PCR Primer, Index 1 (100% over 23bp)
GGTGCTGTAGGCTTTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCGTA21160.23895294343500503RNA PCR Primer, Index 22 (100% over 37bp)
CCACGTTCCCGTGGTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCGTA20880.23579099522319968RNA PCR Primer, Index 22 (100% over 37bp)
TCGTATGACTCTTAGCGGTGGAATTCTCGGGTGCCAAGGAACTCCAGTCAC20740.234210021117297RNA PCR Primer, Index 1 (100% over 33bp)
GAATACCGGGTGCTGTAGGCTTATGGAATTCTCGGGTGCCAAGGAACTCCA20670.23341953406434565RNA PCR Primer, Index 1 (100% over 28bp)
TACTTGGATGGGAGACCGCCTGGAATTCTCGGGTGCCAAGGAACTCCAGTC20660.23330660734249545RNA PCR Primer, Index 1 (100% over 31bp)
TCGTGGTTGTAGTCCGTGCGAGAATACCATGGAATTCTCGGGTGCCAAGGA20620.2328549004550947RNA PCR Primer, Index 1 (100% over 22bp)
AGACCGCCTGGGAATACCGGGTGCTGTAGGCTTTGGAATTCTCGGGTGCCA20170.22777319797183607No Hit
TCCTTCTGATCGAGGCCTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACC20060.22653100403148396RNA PCR Primer, Index 2 (100% over 34bp)
GGGGCCCAAGTCCTTCTGATCGAGGCCTGGAATTCTCGGGTGCCAAGGAAC19980.22562759025668244RNA PCR Primer, Index 1 (100% over 24bp)
TCGACACAAGGGTTTGTTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACC19760.2231432023759782RNA PCR Primer, Index 2 (100% over 34bp)
GTTTGTGATGACTTACATGGAATTCTCGGGTGCCAAGGAACTCCAGTCACC19580.22111052138267476RNA PCR Primer, Index 2 (100% over 34bp)
ATACCGGGTGCTGTAGGCTTTGGAATTCTCGGGTGCCAAGGAACTCCAGTC19580.22111052138267476RNA PCR Primer, Index 1 (100% over 31bp)
GAATACCGGGTGCTGTAGGCTTGGAATTCTCGGGTGCCAAGGAACTCCAGT19290.2178356464490192RNA PCR Primer, Index 1 (100% over 30bp)
CTCGGGTGCCAAGGAACTCCAGTCACCGTACGATCTCGTATGCCGTCTTCT19170.21648052578681692RNA PCR Primer, Index 22 (100% over 51bp)
GGTGCTGTAGGCTTTTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCGT19050.21512540512461462RNA PCR Primer, Index 12 (97% over 36bp)
GGAATACCGGGTGCTGTAGGCTTATGGAATTCTCGGGTGCCAAGGAACTCC18100.20439736654884644RNA PCR Primer, Index 1 (100% over 27bp)
CTCGACACAAGGGTTTGTTGGAATTCTCGGGTGCCAAGGAACTCCAGTCAC17180.19400810813862884RNA PCR Primer, Index 1 (100% over 33bp)
TTCGCGCTTTCCCCTGTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCG17000.1919754271453254RNA PCR Primer, Index 2 (100% over 35bp)
TTAGTACTTGGATGGGAGACCGCCTGGAATTCTCGGGTGCCAAGGAACTCC16310.18418348333766218RNA PCR Primer, Index 1 (100% over 27bp)
AATACCGGGTGCTGTAGGCTTGGAATTCTCGGGTGCCAAGGAACTCCAGTC16050.18124738856955722RNA PCR Primer, Index 1 (100% over 31bp)
GCATTGTGGTTCAGTGGTAGAATTCTCGCCTGCTGGAATTCTCGGGTGCCA15990.18056982823845608No Hit
GTCTGAGCGTCGCTTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCGTA15710.1774078800266507RNA PCR Primer, Index 22 (100% over 37bp)
GCATTGGTGGTTCAGTGGTAGAATTCTCGCCTTGGAATTCTCGGGTGCCAA15610.17627861280814877No Hit
ATACCGGGTGCTGTAGGCTTTTGGAATTCTCGGGTGCCAAGGAACTCCAGT15290.17266495770894266RNA PCR Primer, Index 1 (100% over 30bp)
CCCCACGTTGGGCGCCATGGAATTCTCGGGTGCCAAGGAACTCCAGTCACC14470.16340496651722697RNA PCR Primer, Index 2 (100% over 34bp)
GTCTACGGCCATACCACCCTGAACGCGCCCGATGGAATTCTCGGGTGCCAA14230.16069472519282238No Hit
ACCGGGTGCTGTAGGCTTTGGAATTCTCGGGTGCCAAGGAACTCCAGTCAC14020.15832326403396835RNA PCR Primer, Index 1 (100% over 33bp)
TGTCTGAGCGTCGCTTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCGT14010.15821033731211817RNA PCR Primer, Index 12 (97% over 36bp)
CGTTCGCGCTTTCCCCTGTGGAATTCTCGGGTGCCAAGGAACTCCAGTCAC13970.1577586304247174RNA PCR Primer, Index 1 (100% over 33bp)
CAAGGGTTTGTTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCGTACGA13750.15527424254401317RNA PCR Primer, Index 22 (100% over 40bp)
CGCTTTCCCCTGTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCGTACG13710.15482253565661241RNA PCR Primer, Index 22 (100% over 39bp)
ATGGGAGACCGCCTGGGAATACCGGGTGCTGTAGGCTTTGGAATTCTCGGG13610.15369326843811051No Hit
CGCGACCTCAGATCAGACGTGGCGACCCGCTGATGGAATTCTCGGGTGCCA13450.15188644088850745No Hit
ACCGCCTGGGAATACCGGGTGCTGTAGGCTTTGGAATTCTCGGGTGCCAAG13450.15188644088850745No Hit
TCCAGGGTTCAAGTCCCTGTTCGGGCGCCATGGAATTCTCGGGTGCCAAGG12810.1446591306900952Illumina Small RNA Adapter 2 (100% over 21bp)
CATTATTACTTTTGGTACGCGTGGAATTCTCGGGTGCCAAGGAACTCCAGT12670.1430781565841925RNA PCR Primer, Index 1 (100% over 30bp)
TGGGGGGCCCAAGTCCTTCTGATCGAGGCCTGGAATTCTCGGGTGCCAAGG12420.14025498853793775Illumina Small RNA Adapter 2 (100% over 21bp)
TCCCTGTTCGGGCGCCATGGAATTCTCGGGTGCCAAGGAACTCCAGTCACC12390.13991620837238716RNA PCR Primer, Index 2 (100% over 34bp)
GCCCGGCTAGCTCAGTCGGTAGAGCATGGGACTCTGGAATTCTCGGGTGCC12210.13788352737908371No Hit
TACCGGGTGCTGTAGGCTTTGGAATTCTCGGGTGCCAAGGAACTCCAGTCA12130.1369801136042822RNA PCR Primer, Index 1 (100% over 32bp)
TCTCGGGTGCCAAGGAACTCCAGTCACCGTACGATCTCGTATGCCGTCTTC12090.1365284067168814RNA PCR Primer, Index 22 (100% over 51bp)
TTCGGCACCATGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCGTACGAT11870.1340440188361772RNA PCR Primer, Index 22 (100% over 41bp)
CGTACGACTCTTAGCGGTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACC11840.1337052386706266RNA PCR Primer, Index 2 (100% over 34bp)
CACAAGGGTTTGTTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCGTAC11820.13347938522692623RNA PCR Primer, Index 22 (100% over 38bp)
CCGGGTGCTGTAGGCTTTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACC11820.13347938522692623RNA PCR Primer, Index 2 (100% over 34bp)
GGGAATACCGGGTGCTGTAGGCTTTGGAATTCTCGGGTGCCAAGGAACTCC11810.13336645850507606RNA PCR Primer, Index 1 (100% over 27bp)
GTCTACGGCCATACCACCCTGAACGCGCCCGATCTGGAATTCTCGGGTGCC11700.13212426456472395No Hit
CTGGGAATACCGGGTGCTGTAGGCTTTTGGAATTCTCGGGTGCCAAGGAAC11690.13201133784287375RNA PCR Primer, Index 1 (100% over 24bp)
TTTCCCGGCCAATGCACCATGGAATTCTCGGGTGCCAAGGAACTCCAGTCA11600.13099499734622203RNA PCR Primer, Index 1 (100% over 32bp)
AACATTCAACGCTGTCGGTGAGTGGAATTCTCGGGTGCCAAGGAACTCCAG11370.12839768274366764RNA PCR Primer, Index 1 (100% over 29bp)
TCCTTCATTCCACCGGAGTCTGTTGGAATTCTCGGGTGCCAAGGAACTCCA11350.12817182929996726RNA PCR Primer, Index 1 (100% over 28bp)
GTCTACGGCCATACCACCCTGAACGCGCCCGTGGAATTCTCGGGTGCCAAG11240.12692963535961516No Hit
CGGGTGCTGTAGGCTTTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCG11150.12591329486296343RNA PCR Primer, Index 2 (100% over 35bp)
CAGCTAGGAATAATGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCGTAC11080.1251228078100121RNA PCR Primer, Index 22 (100% over 38bp)
CTCGTACGACTCTTAGCGGTGGAATTCTCGGGTGCCAAGGAACTCCAGTCA11060.12489695436631171RNA PCR Primer, Index 1 (100% over 32bp)
AGTCACGGCACCATGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCGTAC11060.12489695436631171RNA PCR Primer, Index 22 (100% over 38bp)
TCCCTGGTGGTCTAGTGGTTAGGATTCGGCGCTCTGGAATTCTCGGGTGCC11040.12467110092261131No Hit
TGAGGTAGTAGGTTGTATAGTTTGGAATTCTCGGGTGCCAAGGAACTCCAG10860.12263841992930787RNA PCR Primer, Index 1 (100% over 29bp)
GCCCGGCTAGCTCAGTCGGTAGAGCATGAGACTCTGGAATTCTCGGGTGCC10800.12196085959820671No Hit
TGAGATGAAGCACTGTAGCTTGGAATTCTCGGGTGCCAAGGAACTCCAGTC10760.12150915271080596RNA PCR Primer, Index 1 (100% over 31bp)
TGAGGTAGTAGATTGTATAGTTTGGAATTCTCGGGTGCCAAGGAACTCCAG10610.11981525188305309RNA PCR Primer, Index 1 (100% over 29bp)
GTTAGTACTTGGATGGGAGACCGCCTGGAATTCTCGGGTGCCAAGGAACTC10550.11913769155195195RNA PCR Primer, Index 1 (100% over 26bp)
ACCGGGTGCTGTAGGCTTTTGGAATTCTCGGGTGCCAAGGAACTCCAGTCA10540.11902476483010174RNA PCR Primer, Index 1 (100% over 32bp)
TCACGGCACCATGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCGTACGA10500.118573057942701RNA PCR Primer, Index 22 (100% over 40bp)
TGAGGTAGTAGTTTGTGCTGTTTGGAATTCTCGGGTGCCAAGGAACTCCAG10460.11812135105530022RNA PCR Primer, Index 1 (100% over 29bp)
GGGAATACCGGGTGCTGTAGGCTTTTGGAATTCTCGGGTGCCAAGGAACTC10370.1171050105586485RNA PCR Primer, Index 1 (100% over 26bp)
TCGTGGGTTCGAGCCCCACGTTGGGCGCCATGGAATTCTCGGGTGCCAAGG10280.11608867006199677Illumina Small RNA Adapter 2 (100% over 21bp)
GTCTACGGCCATACCACCCTGAACGCGCCCGATTGGAATTCTCGGGTGCCA10090.11394306234684312No Hit
GTGCTGTAGGCTTTTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCGTA10070.11371720890314276RNA PCR Primer, Index 22 (100% over 37bp)
TAGCTTATCAGACTGATGTTGTGGAATTCTCGGGTGCCAAGGAACTCCAGT10050.11349135545944238RNA PCR Primer, Index 1 (100% over 30bp)
ATGGGAGACCGCCTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCGTAC9870.11145867446613893RNA PCR Primer, Index 22 (100% over 38bp)
AAGCTGCCAGTTGAAGAACTGTTGGAATTCTCGGGTGCCAAGGAACTCCAG9810.11078111413503777RNA PCR Primer, Index 1 (100% over 29bp)
CGACACAAGGGTTTGTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCGT9750.11010355380393663RNA PCR Primer, Index 12 (97% over 36bp)
ATACCGGGTGCTGTAGGCTTATGGAATTCTCGGGTGCCAAGGAACTCCAGT9510.10739331247953203RNA PCR Primer, Index 1 (100% over 30bp)
TTTCGGCACCATGGAATTCTCGGGTGCCACGGAACTCCAGTCACCGTACGA9430.10648989870473051RNA PCR Primer, Index 22 (97% over 40bp)
TACCGGGTGCTGTAGGCTTTTGGAATTCTCGGGTGCCAAGGAACTCCAGTC9230.10423136426772667RNA PCR Primer, Index 1 (100% over 31bp)
CTGTCTGAGCGTCGCTTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCG9130.10310209704922477RNA PCR Primer, Index 2 (100% over 35bp)
GGAATTCTCGGGTGCCAAGGAACTCCAGTCACCGTACGATCTCGTATGCCG9010.10174697638702247RNA PCR Primer, Index 22 (100% over 51bp)
TTGGGCGCCATGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCGTACGAT8920.10073063589037075RNA PCR Primer, Index 22 (100% over 41bp)
ATCGAGGCCTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCGTACGATC8910.10061770916852054RNA PCR Primer, Index 22 (100% over 42bp)
TCCAGGGTTCAGGTCCCTGTTCGGGCGCCATGGAATTCTCGGGTGCCAAGG8910.10061770916852054Illumina Small RNA Adapter 2 (100% over 21bp)

[FAIL]Adapter Content

Adapter graph