FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005732235

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005732235
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6024736
Sequences flagged as poor quality0
Sequence length51
%GC53

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TGGAATTCTCGGGTGCCAAGGAACTCCAGTCACGATCAGATCTCGTATGCC5532889.183605721478916RNA PCR Primer, Index 9 (100% over 51bp)
TCGTACGACTCTTAGCGGTGGAATTCTCGGGTGCCAAGGAACTCCAGTCAC1163921.9319020783649277RNA PCR Primer, Index 1 (100% over 33bp)
TCCTTCATTCCACCGGAGTCTGTGGAATTCTCGGGTGCCAAGGAACTCCAG1032951.714514959659643RNA PCR Primer, Index 1 (100% over 29bp)
GGAATACCGGGTGCTGTAGGCTTTTGGAATTCTCGGGTGCCAAGGAACTCC705591.171155051441258RNA PCR Primer, Index 1 (100% over 27bp)
GGAATACCGGGTGCTGTAGGCTTTGGAATTCTCGGGTGCCAAGGAACTCCA643151.067515655457766RNA PCR Primer, Index 1 (100% over 28bp)
TAGCTTATCAGACTGATGTTGATGGAATTCTCGGGTGCCAAGGAACTCCAG424990.7054085025468335RNA PCR Primer, Index 1 (100% over 29bp)
GAATACCGGGTGCTGTAGGCTTTGGAATTCTCGGGTGCCAAGGAACTCCAG413940.6870674499264366RNA PCR Primer, Index 1 (100% over 29bp)
ACACAAGGGTTTGTTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACGATC412180.6841461600973056RNA PCR Primer, Index 9 (100% over 37bp)
TCCTTCATTCCACCGGAGTCTGTTGGAATTCTCGGGTGCCAAGGAACTCCA393350.6528916785731359RNA PCR Primer, Index 1 (100% over 28bp)
GAATACCGGGTGCTGTAGGCTTTTGGAATTCTCGGGTGCCAAGGAACTCCA380850.6321438814912388RNA PCR Primer, Index 1 (100% over 28bp)
AATACCGGGTGCTGTAGGCTTTGGAATTCTCGGGTGCCAAGGAACTCCAGT366310.6080100439255762RNA PCR Primer, Index 1 (100% over 30bp)
GCATTGGTGGTTCAGTGGTAGAATTCTCGCCTGCTGGAATTCTCGGGTGCC322260.534894807008971No Hit
TAGCTTATCAGACTGATGTTGACTGGAATTCTCGGGTGCCAAGGAACTCCA314530.522064369293526RNA PCR Primer, Index 1 (100% over 28bp)
TGAGATGAAGCACTGTAGCTTGGAATTCTCGGGTGCCAAGGAACTCCAGTC304440.5053167474890186RNA PCR Primer, Index 1 (100% over 31bp)
TTCACAGTGGCTAAGTTCTGTGGAATTCTCGGGTGCCAAGGAACTCCAGTC290250.48176384824164914RNA PCR Primer, Index 1 (100% over 31bp)
TCTGAGCGTCGCTTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACGATCA277580.46073388111943825RNA PCR Primer, Index 9 (100% over 38bp)
CCTGTCTGAGCGTCGCTTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACG265450.44060021883116535RNA PCR Primer, Index 6 (100% over 34bp)
TGAGGTAGTAGATTGTATAGTTTGGAATTCTCGGGTGCCAAGGAACTCCAG259450.43064127623185483RNA PCR Primer, Index 1 (100% over 29bp)
GCATTGGTGGTTCAGTGGTAGAATTCTCGCCTTGGAATTCTCGGGTGCCAA258880.42969517668492035No Hit
AATACCGGGTGCTGTAGGCTTTTGGAATTCTCGGGTGCCAAGGAACTCCAG231030.3834690847864537RNA PCR Primer, Index 1 (100% over 29bp)
TATTGCACTTGTCCCGGCCTGTTGGAATTCTCGGGTGCCAAGGAACTCCAG190370.315980650438459RNA PCR Primer, Index 1 (100% over 29bp)
TAGCTTATCAGACTGATGTTGTGGAATTCTCGGGTGCCAAGGAACTCCAGT188740.31327513769897963RNA PCR Primer, Index 1 (100% over 30bp)
GTACGACTCTTAGCGGTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACGA188140.3122792434390486RNA PCR Primer, Index 7 (97% over 35bp)
TCGTGGTTGTAGTCCGTGCGAGAATACCATGGAATTCTCGGGTGCCAAGGA187430.3111007685647969RNA PCR Primer, Index 1 (100% over 22bp)
TGAGGTAGTAGGTTGTATAGTTTGGAATTCTCGGGTGCCAAGGAACTCCAG187230.31076880381148647RNA PCR Primer, Index 1 (100% over 29bp)
CAAGGGTTTGTTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACGATCAGA185820.3084284523006485RNA PCR Primer, Index 9 (100% over 40bp)
TGAGAACTGAATTCCATAGGCTGTTGGAATTCTCGGGTGCCAAGGAACTCC183240.304146106982945RNA PCR Primer, Index 1 (100% over 27bp)
TTTCGGCACCATGGAATTCTCGGGTGCCAAGGAACTCCAGTCACGATCAGA178210.2957971934371896RNA PCR Primer, Index 9 (100% over 40bp)
GTAGTCGTGGCCTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACGATCAG176320.2926601265184068RNA PCR Primer, Index 9 (100% over 39bp)
TTCAAGTAATCCAGGATAGGCTTGGAATTCTCGGGTGCCAAGGAACTCCAG165720.2750659945929581RNA PCR Primer, Index 1 (100% over 29bp)
CCACGTTCCCGTGGTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACGATC159100.2640779612583854RNA PCR Primer, Index 9 (100% over 37bp)
CGCGACCTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACGATCAGATCTC158300.262750102245144RNA PCR Primer, Index 9 (100% over 44bp)
GTCTGAGCGTCGCTTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACGATC154310.2561274054166025RNA PCR Primer, Index 9 (100% over 37bp)
AGGGTTTGTTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACGATCAGATC150240.2493719226867368RNA PCR Primer, Index 9 (100% over 42bp)
TCGACACAAGGGTTTGTTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACG150220.24933872621140576RNA PCR Primer, Index 6 (100% over 34bp)
TGGGAATACCGGGTGCTGTAGGCTTTTGGAATTCTCGGGTGCCAAGGAACT149580.24827643900081267RNA PCR Primer, Index 1 (100% over 25bp)
GGTGCTGTAGGCTTTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACGATC144240.23941298008742623RNA PCR Primer, Index 9 (100% over 37bp)
TAGCAGCACGTAAATATTGGCGTGGAATTCTCGGGTGCCAAGGAACTCCAG140930.2339189634201399RNA PCR Primer, Index 1 (100% over 29bp)
TGGGAATACCGGGTGCTGTAGGCTTTGGAATTCTCGGGTGCCAAGGAACTC140900.23386916870714336RNA PCR Primer, Index 1 (100% over 26bp)
AAGGGTTTGTTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACGATCAGAT139050.2307984947390226RNA PCR Primer, Index 9 (100% over 41bp)
CTCGACACAAGGGTTTGTTGGAATTCTCGGGTGCCAAGGAACTCCAGTCAC135450.22482312917943623RNA PCR Primer, Index 1 (100% over 33bp)
CACAAGGGTTTGTTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACGATCA133710.22193503582563617RNA PCR Primer, Index 9 (100% over 38bp)
GAGACCGCCTGGGAATACCGGGTGCTGTAGGCTTTGGAATTCTCGGGTGCC132610.22010922968242924No Hit
ATACCGGGTGCTGTAGGCTTTGGAATTCTCGGGTGCCAAGGAACTCCAGTC129810.2154617231360843RNA PCR Primer, Index 1 (100% over 31bp)
TACCCTGTAGAACCGAATTTGTTGGAATTCTCGGGTGCCAAGGAACTCCAG129440.21484758834246018RNA PCR Primer, Index 1 (100% over 29bp)
CCTGGGAATACCGGGTGCTGTAGGCTTTGGAATTCTCGGGTGCCAAGGAAC129390.21476459715413257RNA PCR Primer, Index 1 (100% over 24bp)
TGAGGTAGTAGTTTGTGCTGTTTGGAATTCTCGGGTGCCAAGGAACTCCAG129340.214681605965805RNA PCR Primer, Index 1 (100% over 29bp)
TGTCTGAGCGTCGCTTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACGAT128920.21398447998385323RNA PCR Primer, Index 9 (100% over 36bp)
GTCTACGGCCATACCACCCTGAACGCGCCCGATGGAATTCTCGGGTGCCAA127580.21176031613667384No Hit
TTCACAGTGGCTAAGTTCTGCTGGAATTCTCGGGTGCCAAGGAACTCCAGT126560.21006729589479106RNA PCR Primer, Index 1 (100% over 30bp)
CGACACAAGGGTTTGTTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACGA125720.2086730439308876RNA PCR Primer, Index 7 (97% over 35bp)
GGAATACCGGGTGCTGTAGGCTTGGAATTCTCGGGTGCCAAGGAACTCCAG123850.2055691734874358RNA PCR Primer, Index 1 (100% over 29bp)
CTTTCCCCTGTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACGATCAGAT120380.19980958501750115RNA PCR Primer, Index 9 (100% over 41bp)
GTCTACGGCCTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACGATCAGAT117990.19584260621544244RNA PCR Primer, Index 9 (100% over 41bp)
CATTGCACTTGTCTCGGTCTGATGGAATTCTCGGGTGCCAAGGAACTCCAG117430.1949131049061735RNA PCR Primer, Index 1 (100% over 29bp)
TCAGTGCACTACAGAACTTTGTTGGAATTCTCGGGTGCCAAGGAACTCCAG115080.19101251905477684RNA PCR Primer, Index 1 (100% over 29bp)
AATACCGGGTGCTGTAGGCTTATGGAATTCTCGGGTGCCAAGGAACTCCAG114080.18935269528822507RNA PCR Primer, Index 1 (100% over 29bp)
GGTGCTGTAGGCTTTTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACGAT113650.18863897106860783RNA PCR Primer, Index 9 (100% over 36bp)
AACATTCAACGCTGTCGGTGAGTGGAATTCTCGGGTGCCAAGGAACTCCAG112790.1872115226293733RNA PCR Primer, Index 1 (100% over 29bp)
CAACGGAATCCCAAAAGCAGCTGTGGAATTCTCGGGTGCCAAGGAACTCCA111070.18435662575090428RNA PCR Primer, Index 1 (100% over 28bp)
GTTTCCGTAGTGTAGTGGTTATCACGTTCGCCTTGGAATTCTCGGGTGCCA110190.18289598083633873No Hit
AATACCGGGTGCTGTAGGCTTGGAATTCTCGGGTGCCAAGGAACTCCAGTC109130.18113656764379385RNA PCR Primer, Index 1 (100% over 31bp)
CGCTTTCCCCTGTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACGATCAG106660.177036802940411RNA PCR Primer, Index 9 (100% over 39bp)
GAATACCGGGTGCTGTAGGCTTGGAATTCTCGGGTGCCAAGGAACTCCAGT106420.17663844523643857RNA PCR Primer, Index 1 (100% over 30bp)
CCTGGGAATACCGGGTGCTGTAGGCTTTTGGAATTCTCGGGTGCCAAGGAA105930.17582513159082822RNA PCR Primer, Index 1 (100% over 23bp)
ACACAAGGGTTTGTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACGATCA104810.17396612897229025RNA PCR Primer, Index 9 (100% over 38bp)
TCGCTGGTTCGTTTCCGGCTCGAAGGACCATGGAATTCTCGGGTGCCAAGG104580.17358436950598333Illumina Small RNA Adapter 2 (100% over 21bp)
GCATTGTGGTTCAGTGGTAGAATTCTCGCCTGCTGGAATTCTCGGGTGCCA103110.17114442856915224No Hit
ACAAGGGTTTGTTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACGATCAG102360.16989956074423843RNA PCR Primer, Index 9 (100% over 39bp)
TTCGGGCGCCATGGAATTCTCGGGTGCCAAGGAACTCCAGTCACGATCAGA101270.168090352838697RNA PCR Primer, Index 9 (100% over 40bp)
GGAATACCGGGTGCTGTAGGCTTATGGAATTCTCGGGTGCCAAGGAACTCC100500.16681228853845212RNA PCR Primer, Index 1 (100% over 27bp)
TATTGCACTTGTCCCGGCCTGTAATGGAATTCTCGGGTGCCAAGGAACTCC98040.1627291220727348RNA PCR Primer, Index 1 (100% over 27bp)
GGGAATACCGGGTGCTGTAGGCTTTTGGAATTCTCGGGTGCCAAGGAACTC97440.16173322781280375RNA PCR Primer, Index 1 (100% over 26bp)
TTGTACGACTCTTAGCGGTGGAATTCTCGGGTGCCAAGGAACTCCAGTCAC96740.1605713511762175RNA PCR Primer, Index 1 (100% over 33bp)
GAATACCGGGTGCTGTAGGCTTATGGAATTCTCGGGTGCCAAGGAACTCCA95760.15894472388499678RNA PCR Primer, Index 1 (100% over 28bp)
GGGAATACCGGGTGCTGTAGGCTTTGGAATTCTCGGGTGCCAAGGAACTCC94670.15713551597945535RNA PCR Primer, Index 1 (100% over 27bp)
TTCGCGCTTTCCCCTGTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACGA91820.15240501824478284RNA PCR Primer, Index 7 (97% over 35bp)
GTCTACGGCCATACCACCCTGAACGCGCCCGTGGAATTCTCGGGTGCCAAG88960.1476579222724448No Hit
ATACCGGGTGCTGTAGGCTTTTGGAATTCTCGGGTGCCAAGGAACTCCAGT88590.14704378747882066RNA PCR Primer, Index 1 (100% over 30bp)
TAGCTTATCAGACTGATGTTGACTTGGAATTCTCGGGTGCCAAGGAACTCC86000.1427448439234516RNA PCR Primer, Index 1 (100% over 27bp)
CCGGGTGCTGTAGGCTTTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACG82770.13738361315748937RNA PCR Primer, Index 6 (100% over 34bp)
GCATTGTGGTTCAGTGGTAGAATTCTCGCCTTGGAATTCTCGGGTGCCAAG82770.13738361315748937No Hit
CGTTCGCGCTTTCCCCTGTGGAATTCTCGGGTGCCAAGGAACTCCAGTCAC81980.1360723523819135RNA PCR Primer, Index 1 (100% over 33bp)
GTGCTGTAGGCTTTTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACGATC81750.1356905929156066RNA PCR Primer, Index 9 (100% over 37bp)
AGACCGCCTGGGAATACCGGGTGCTGTAGGCTTTGGAATTCTCGGGTGCCA81630.1354914140636204No Hit
TGAGAACTGAATTCCATAGGCTGTGGAATTCTCGGGTGCCAAGGAACTCCA80140.13301827665145827RNA PCR Primer, Index 1 (100% over 28bp)
TACCGGGTGCTGTAGGCTTTGGAATTCTCGGGTGCCAAGGAACTCCAGTCA79780.13242074009549962RNA PCR Primer, Index 1 (100% over 32bp)
GTGCTGTAGGCTTTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACGATCA79690.13227135595650996RNA PCR Primer, Index 9 (100% over 38bp)
CGGGGCTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACGATCAGATCTCG79530.13200578415386166RNA PCR Primer, Index 9 (100% over 45bp)
CTGTCTGAGCGTCGCTTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACGA79050.13120906874591684RNA PCR Primer, Index 7 (97% over 35bp)
TCAGTGCACTACAGAACTTTGTCTGGAATTCTCGGGTGCCAAGGAACTCCA78190.1297816203066823RNA PCR Primer, Index 1 (100% over 28bp)
AAGCTGCCAGTTGAAGAACTGTTGGAATTCTCGGGTGCCAAGGAACTCCAG77650.12888531547274437RNA PCR Primer, Index 1 (100% over 29bp)
CGGGTGCTGTAGGCTTTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACGA77550.1287193330960892RNA PCR Primer, Index 7 (97% over 35bp)
GTCTACGGCCATACCACCCTGAACGCGCCCGATTGGAATTCTCGGGTGCCA76760.1274080723205133No Hit
GGGGCTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACGATCAGATCTCGT76300.12664455338789948RNA PCR Primer, Index 9 (100% over 46bp)
CACCACGTTCCCGTGGTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACGA76110.12632918687225464RNA PCR Primer, Index 7 (97% over 35bp)
TCCTTCATTCCACCGGAGTCTGATGGAATTCTCGGGTGCCAAGGAACTCCA75380.12511751552267186RNA PCR Primer, Index 1 (100% over 28bp)
GCATTGGTGGTTCAGTGGTAGAATTCTCGCCTGGAATTCTCGGGTGCCAAG75190.12480214900702703No Hit
CGCGACCTTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACGATCAGATCT74260.12325851290413388RNA PCR Primer, Index 9 (100% over 43bp)
CTGGGAATACCGGGTGCTGTAGGCTTTTGGAATTCTCGGGTGCCAAGGAAC74230.12320871819113734RNA PCR Primer, Index 1 (100% over 24bp)
GTCTACGGCCATACCACCCTGAACGCGCCCGATCTGGAATTCTCGGGTGCC73950.12274396753650285No Hit
TATTGCACTTGTCCCGGCCTGTATGGAATTCTCGGGTGCCAAGGAACTCCA71880.11930813233974069RNA PCR Primer, Index 1 (100% over 28bp)
TCCAGGGTTCAAGTCCCTGTTCGGGCGCCATGGAATTCTCGGGTGCCAAGG71880.11930813233974069Illumina Small RNA Adapter 2 (100% over 21bp)
CATTATTACTTTTGGTACGCGTGGAATTCTCGGGTGCCAAGGAACTCCAGT71390.11849481869413032RNA PCR Primer, Index 1 (100% over 30bp)
GTTTCGGCACCATGGAATTCTCGGGTGCCAAGGAACTCCAGTCACGATCAG70880.11764830857318893RNA PCR Primer, Index 9 (100% over 39bp)
GTCTACGGCCATACCACCCTGAACGCGCCCTGGAATTCTCGGGTGCCAAGG69340.11509217997269922Illumina Small RNA Adapter 2 (100% over 21bp)
GTTTGTGATGACTTACATGGAATTCTCGGGTGCCAAGGAACTCCAGTCACG69060.11462742931806472RNA PCR Primer, Index 6 (100% over 34bp)
TGTAAACATCCTCGACTGGAAGCTTGGAATTCTCGGGTGCCAAGGAACTCC67920.11273523022419572RNA PCR Primer, Index 1 (100% over 27bp)
CTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACGATCAGATCTCGTATGC67490.11202150600457847RNA PCR Primer, Index 9 (100% over 50bp)
GTCTACGGCCATACCACCCTGAACGCGCCTGGAATTCTCGGGTGCCAAGGA66310.11006291396004739RNA PCR Primer, Index 1 (100% over 22bp)
CTGAGCGTCGCTTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACGATCAG65850.10929939502743356RNA PCR Primer, Index 9 (100% over 39bp)
CCCCACGTTGGGCGCCATGGAATTCTCGGGTGCCAAGGAACTCCAGTCACG64850.1076395712608818RNA PCR Primer, Index 6 (100% over 34bp)
ACTGGACTTGGAGTCAGAAGGCTGGAATTCTCGGGTGCCAAGGAACTCCAG64790.1075399818348887RNA PCR Primer, Index 1 (100% over 29bp)
CTGGGAATACCGGGTGCTGTAGGCTTTGGAATTCTCGGGTGCCAAGGAACT64520.10709182941791973RNA PCR Primer, Index 1 (100% over 25bp)
GGAATTCTCGGGTGCCAAGGAACTCCAGTCACGATCAGATCTCGTATGCCG64430.10694244527893007RNA PCR Primer, Index 9 (100% over 51bp)
CGCGACCTCAGATCAGACGTGGCTGGAATTCTCGGGTGCCAAGGAACTCCA63970.10617892634631626RNA PCR Primer, Index 1 (100% over 28bp)
TGAGCGTCGCTTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACGATCAGA63480.1053656127007059RNA PCR Primer, Index 9 (100% over 40bp)
TGAGGTAGTAGATTGTATAGTTGGAATTCTCGGGTGCCAAGGAACTCCAGT61780.10254391229756789RNA PCR Primer, Index 1 (100% over 30bp)

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATTACAT406.7975634E-945.0190621
CACCGAC302.1607339E-645.0190621
GGGGGTA3100.045.0190621
AATAGCG302.1643173E-645.008232
AATAACG207.0293405E-445.008232
CGTAAGA700.045.0082242
GACGCCC207.032503E-445.0041163
ATACGAC351.2122109E-745.0041163
CGTTACC1800.045.0007555
GCACATC650.045.0007556
TCAACGT551.8189894E-1245.0007556
GTTAATC207.0350914E-445.000756
TAGACCA207.0359546E-444.99963420
AAACGCG207.0359546E-444.99963421
AATGCGC551.8189894E-1244.99963422
ATGCGCC551.8189894E-1244.99963423
AACTCGC253.8929706E-544.99963422
TCATTAG253.8937338E-544.9981429
ATAGACC302.1676587E-644.99814213
CATACTA302.1676587E-644.99814210
Produced by FastQC (version 0.11.5)