FastQCFastQC Report
Wed 27 Apr 2022
EGAF00005732237

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005732237
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1753914
Sequences flagged as poor quality0
Sequence length51
%GC53

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TCGTACGACTCTTAGCGGTGGAATTCTCGGGTGCCAAGGAACTCCAGTCAC1292217.3675790261096035RNA PCR Primer, Index 1 (100% over 33bp)
TGGAATTCTCGGGTGCCAAGGAACTCCAGTCACTACAGCATCTCGTATGCC816904.65758298297408RNA PCR Primer, Index 43 (100% over 51bp)
AATTCTCGGGTGCCAAGGAACTCCAGTCACTACAGCATCTCGTATGCCGTC471782.6898696287275206RNA PCR Primer, Index 43 (100% over 51bp)
TAGCTTATCAGACTGATGTTGACTGGAATTCTCGGGTGCCAAGGAACTCCA435752.48444336495404RNA PCR Primer, Index 1 (100% over 28bp)
TAGCTTATCAGACTGATGTTGATGGAATTCTCGGGTGCCAAGGAACTCCAG369582.1071728716459304RNA PCR Primer, Index 1 (100% over 29bp)
GTACGACTCTTAGCGGTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACTA269341.535651120864535RNA PCR Primer, Index 7 (97% over 35bp)
TACCCTGTAGAACCGAATTTGTTGGAATTCTCGGGTGCCAAGGAACTCCAG210091.197835241636705RNA PCR Primer, Index 1 (100% over 29bp)
CTCGGGTGCCAAGGAACTCCAGTCACTACAGCATCTCGTATGCCGTCTTCT206161.1754282136980492RNA PCR Primer, Index 43 (100% over 51bp)
TCTCGGGTGCCAAGGAACTCCAGTCACTACAGCATCTCGTATGCCGTCTTC164210.9362488696709189RNA PCR Primer, Index 43 (100% over 51bp)
TCCTTCATTCCACCGGAGTCTGTGGAATTCTCGGGTGCCAAGGAACTCCAG137280.7827065637197719RNA PCR Primer, Index 1 (100% over 29bp)
TGAGATGAAGCACTGTAGCTTGGAATTCTCGGGTGCCAAGGAACTCCAGTC120250.6856094426522623RNA PCR Primer, Index 1 (100% over 31bp)
ATTCTCGGGTGCCAAGGAACTCCAGTCACTACAGCATCTCGTATGCCGTCT114590.6533387611935363RNA PCR Primer, Index 43 (100% over 51bp)
TTCACAGTGGCTAAGTTCTGTGGAATTCTCGGGTGCCAAGGAACTCCAGTC106000.6043625856227842RNA PCR Primer, Index 1 (100% over 31bp)
TTGTACGACTCTTAGCGGTGGAATTCTCGGGTGCCAAGGAACTCCAGTCAC105430.6011127113416052RNA PCR Primer, Index 1 (100% over 33bp)
GCATTGGTGGTTCAGTGGTAGAATTCTCGCCTTGGAATTCTCGGGTGCCAA95760.5459788792380926No Hit
TGAGGTAGTAGTTTGTGCTGTTTGGAATTCTCGGGTGCCAAGGAACTCCAG94650.5396501766905333RNA PCR Primer, Index 1 (100% over 29bp)
AAGCTGCCAGTTGAAGAACTGTTGGAATTCTCGGGTGCCAAGGAACTCCAG79280.4520176017752296RNA PCR Primer, Index 1 (100% over 29bp)
TAGCTTATCAGACTGATGTTGTGGAATTCTCGGGTGCCAAGGAACTCCAGT76770.4377067518703882RNA PCR Primer, Index 1 (100% over 30bp)
CATTATTACTTTTGGTACGCGTGGAATTCTCGGGTGCCAAGGAACTCCAGT73460.41883467490424275RNA PCR Primer, Index 1 (100% over 30bp)
AACATTCAACGCTGTCGGTGAGTGGAATTCTCGGGTGCCAAGGAACTCCAG70590.4024712728218145RNA PCR Primer, Index 1 (100% over 29bp)
CAACACCAGTCGATGGGCTGTTGGAATTCTCGGGTGCCAAGGAACTCCAGT60550.3452278732024489RNA PCR Primer, Index 1 (100% over 30bp)
TGAGATGAAGCACTGTAGCTCTGGAATTCTCGGGTGCCAAGGAACTCCAGT53790.30668550453442983RNA PCR Primer, Index 1 (100% over 30bp)
TCGTATGACTCTTAGCGGTGGAATTCTCGGGTGCCAAGGAACTCCAGTCAC51190.29186151658519177RNA PCR Primer, Index 1 (100% over 33bp)
TTCTCGGGTGCCAAGGAACTCCAGTCACTACAGCATCTCGTATGCCGTCTT50900.29020807177546903RNA PCR Primer, Index 43 (100% over 51bp)
GCATTGGTGGTTCAGTGGTAGAATTCTCGCCTGGAATTCTCGGGTGCCAAG49160.2802874029171328No Hit
AGGGTTTGTTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACTACAGCATC49100.27994531088753494RNA PCR Primer, Index 43 (100% over 42bp)
CGCGACCTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACTACAGCATCTC47830.27270436292771477RNA PCR Primer, Index 43 (100% over 44bp)
TGAGGTAGTAGGTTGTATAGTTTGGAATTCTCGGGTGCCAAGGAACTCCAG47670.271792117515454RNA PCR Primer, Index 1 (100% over 29bp)
CGCGACCTCAGATCAGACGTGGCGACCCGCTGATGGAATTCTCGGGTGCCA47070.26837119721947594No Hit
CATTGCACTTGTCTCGGTCTGATGGAATTCTCGGGTGCCAAGGAACTCCAG46690.26620461436535653RNA PCR Primer, Index 1 (100% over 29bp)
GGGGCTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACTACAGCATCTCGT45280.2581654516698082RNA PCR Primer, Index 43 (100% over 46bp)
GGAATTCTCGGGTGCCAAGGAACTCCAGTCACTACAGCATCTCGTATGCCG45240.257937390316743RNA PCR Primer, Index 43 (100% over 51bp)
TGAGATGAAGCACTGTAGCTTTGGAATTCTCGGGTGCCAAGGAACTCCAGT43920.25041136566559136RNA PCR Primer, Index 1 (100% over 30bp)
CGGGGCTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACTACAGCATCTCG43550.24830179814973827RNA PCR Primer, Index 43 (100% over 45bp)
TAGCTTATCAGACTGATGTTGACTTGGAATTCTCGGGTGCCAAGGAACTCC43080.24562207725122212RNA PCR Primer, Index 1 (100% over 27bp)
TGAGATGAAGCACTGTAGCTCTTGGAATTCTCGGGTGCCAAGGAACTCCAG42550.24260026432310822RNA PCR Primer, Index 1 (100% over 29bp)
TTCACAGTGGCTAAGTTCTGCTGGAATTCTCGGGTGCCAAGGAACTCCAGT42510.24237220297004303RNA PCR Primer, Index 1 (100% over 30bp)
CAAGGGTTTGTTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACTACAGCA42310.241231896204717RNA PCR Primer, Index 43 (100% over 40bp)
TCGTGGGTTCGAGCCCCACGTTGGGCGCCATGGAATTCTCGGGTGCCAAGG41080.23421900959796205Illumina Small RNA Adapter 2 (100% over 21bp)
AACATTCAACGCTGTCGGTGAGTTGGAATTCTCGGGTGCCAAGGAACTCCA40890.23313571817090234RNA PCR Primer, Index 1 (100% over 28bp)
GCCCGGCTAGCTCAGTCGGTAGAGCATGGGACTTGGAATTCTCGGGTGCCA39170.22332907998909865No Hit
TGAGAACTGAATTCCATAGGCTGTTGGAATTCTCGGGTGCCAAGGAACTCC37750.215232901955284RNA PCR Primer, Index 1 (100% over 27bp)
GTTTCCGTAGTGTAGTGGTTATCACGTTCGCCTTGGAATTCTCGGGTGCCA35400.20183429746270343No Hit
TAGCTTATCAGACTGATGTTGACATGGAATTCTCGGGTGCCAAGGAACTCC34670.1976721777692635RNA PCR Primer, Index 1 (100% over 27bp)
CAACGGAATCCCAAAAGCAGCTGTGGAATTCTCGGGTGCCAAGGAACTCCA34070.19425125747328545RNA PCR Primer, Index 1 (100% over 28bp)
TCGACACAAGGGTTTGTTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACT33610.19162855191303566RNA PCR Primer, Index 3 (100% over 34bp)
TCGGGTGCCAAGGAACTCCAGTCACTACAGCATCTCGTATGCCGTCTTCTG32300.18415954260015027RNA PCR Primer, Index 43 (100% over 51bp)
TATTGCACTTGTCCCGGCCTGTTGGAATTCTCGGGTGCCAAGGAACTCCAG31660.18051056095110707RNA PCR Primer, Index 1 (100% over 29bp)
TTCGGCACCATGGAATTCTCGGGTGCCAAGGAACTCCAGTCACTACAGCAT31240.17811591674392244RNA PCR Primer, Index 43 (100% over 41bp)
GTTTGTGATGACTTACATGGAATTCTCGGGTGCCAAGGAACTCCAGTCACT31120.17743173268472684RNA PCR Primer, Index 3 (100% over 34bp)
GCATGGGTGGTTCAGTGGTAGAATTCTCGCCTTGGAATTCTCGGGTGCCAA31010.17680456396379754No Hit
CGACACAAGGGTTTGTTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACTA31000.17674754862553124RNA PCR Primer, Index 7 (97% over 35bp)
ACACAAGGGTTTGTTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACTACA30790.17555022652193894RNA PCR Primer, Index 43 (100% over 37bp)
CCACGTTCCCGTGGTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACTACA30790.17555022652193894RNA PCR Primer, Index 43 (100% over 37bp)
TGAGGTAGTAGATTGTATAGTTTGGAATTCTCGGGTGCCAAGGAACTCCAG30350.1730415516382217RNA PCR Primer, Index 1 (100% over 29bp)
GAATACCGGGTGCTGTAGGCTTTTGGAATTCTCGGGTGCCAAGGAACTCCA30180.1720722908876946RNA PCR Primer, Index 1 (100% over 28bp)
CACCCGTAGAACCGACCTTGCGTGGAATTCTCGGGTGCCAAGGAACTCCAG30140.1718442295346294RNA PCR Primer, Index 1 (100% over 29bp)
GCATTGGTGGTTCAGTGGTAGAATTCTCGCCTGCTGGAATTCTCGGGTGCC29940.1707039227693034No Hit
TCCAGGGTTCAAGTCCCTGTTCGGGCGCCATGGAATTCTCGGGTGCCAAGG28700.16363402082428213Illumina Small RNA Adapter 2 (100% over 21bp)
GGAATACCGGGTGCTGTAGGCTTTTGGAATTCTCGGGTGCCAAGGAACTCC28570.16289282142682024RNA PCR Primer, Index 1 (100% over 27bp)
TCGGATCCGTCTGAGCTTGGCTTGGAATTCTCGGGTGCCAAGGAACTCCAG28250.16106833060229864RNA PCR Primer, Index 1 (100% over 29bp)
GAATACCGGGTGCTGTAGGCTTTGGAATTCTCGGGTGCCAAGGAACTCCAG27760.15827457902724992RNA PCR Primer, Index 1 (100% over 29bp)
GTCTACGGCCATACCACCCTGATGGAATTCTCGGGTGCCAAGGAACTCCAG26890.15331424459808177RNA PCR Primer, Index 1 (100% over 29bp)
TACCCTGTAGATCCGAATTTGTTGGAATTCTCGGGTGCCAAGGAACTCCAG26770.15263006053888617RNA PCR Primer, Index 1 (100% over 29bp)
AAGGGTTTGTTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACTACAGCAT26500.15109064640569606RNA PCR Primer, Index 43 (100% over 41bp)
GTAGTCGTGGCCTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACTACAGC26200.14938018625770705RNA PCR Primer, Index 43 (100% over 39bp)
TTTCGGCACCATGGAATTCTCGGGTGCCAAGGAACTCCAGTCACTACAGCA26070.14863898686024515RNA PCR Primer, Index 43 (100% over 40bp)
CACAAGGGTTTGTTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACTACAG25370.14464791318160414RNA PCR Primer, Index 43 (100% over 38bp)
TCCCTGGTGGTCTAGTGGTTAGGATTCGGCGCTGGAATTCTCGGGTGCCAA25090.1430514837101477No Hit
TGAGGTAGTAGGTTGTGTGGTTTGGAATTCTCGGGTGCCAAGGAACTCCAG24830.1415690849152239RNA PCR Primer, Index 1 (100% over 29bp)
CCGGGTGCTGTAGGCTTTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACT24580.1401437014585664RNA PCR Primer, Index 3 (100% over 34bp)
ACGGCACCATGGAATTCTCGGGTGCCAAGGAACTCCAGTCACTACAGCATC24480.1395735480759034RNA PCR Primer, Index 43 (100% over 42bp)
TAGCAGCACGTAAATATTGGCGTGGAATTCTCGGGTGCCAAGGAACTCCAG24300.13854727198710998RNA PCR Primer, Index 1 (100% over 29bp)
TCCCTGGTGGTCTAGTGGTTAGGATTCGGCGCTTGGAATTCTCGGGTGCCA23720.13524038236766453No Hit
CTCGACACAAGGGTTTGTTGGAATTCTCGGGTGCCAAGGAACTCCAGTCAC23570.13438515229367004RNA PCR Primer, Index 1 (100% over 33bp)
CCGGGTGCTGTAGGCTTTTGGAATTCTCGGGTGCCAAGGAACTCCAGTCAC23390.13335887620487666RNA PCR Primer, Index 1 (100% over 33bp)
CGGGTGCTGTAGGCTTTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACTA23300.13284573816047993RNA PCR Primer, Index 7 (97% over 35bp)
GTCTACGGCCATACCACCCTTGGAATTCTCGGGTGCCAAGGAACTCCAGTC23250.13256066146914844RNA PCR Primer, Index 1 (100% over 31bp)
CATGGAATTCTCGGGTGCCAAGGAACTCCAGTCACTACAGCATCTCGTATG23240.13250364613088214RNA PCR Primer, Index 43 (100% over 49bp)
GGGGGCTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACTACAGCATCTCG23100.13170543139515392RNA PCR Primer, Index 43 (100% over 45bp)
TTCAAGTAATCCAGGATAGGCTTGGAATTCTCGGGTGCCAAGGAACTCCAG22910.13062213996809421RNA PCR Primer, Index 1 (100% over 29bp)
CTCGGGTGCCAAGGAACTCCAGTCACTACCGCATCTCGTATGCCGTCTTCT22750.1297098945558334RNA PCR Primer, Index 43 (98% over 51bp)
CTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACTACAGCATCTCGTATGC22670.12925377184970302RNA PCR Primer, Index 43 (100% over 50bp)
TCCTTCATTCCACCGGAGTCTGTTGGAATTCTCGGGTGCCAAGGAACTCCA22290.12708718899558358RNA PCR Primer, Index 1 (100% over 28bp)
GGAATACCGGGTGCTGTAGGCTTTGGAATTCTCGGGTGCCAAGGAACTCCA22220.1266880816277195RNA PCR Primer, Index 1 (100% over 28bp)
GACACAAGGGTTTGTTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACTAC21910.12492060614146416RNA PCR Primer, Index 30 (97% over 36bp)
CTCGTACGACTCTTAGCGGTGGAATTCTCGGGTGCCAAGGAACTCCAGTCA21450.12229790058121437RNA PCR Primer, Index 1 (100% over 32bp)
CTCGTCGCCATGGAATTCTCGGGTGCCAAGGAACTCCAGTCACTACAGCAT21450.12229790058121437RNA PCR Primer, Index 43 (100% over 41bp)
TCGCTGGTTCGTTTCCGGCTCGAAGGACCATGGAATTCTCGGGTGCCAAGG21320.12155670118375245Illumina Small RNA Adapter 2 (100% over 21bp)
GCATTGTGGTTCAGTGGTAGAATTCTCGCCTTGGAATTCTCGGGTGCCAAG20960.11950414900616564No Hit
CGGGTGCTGTAGGCTTTTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACT20940.11939011832963305RNA PCR Primer, Index 3 (100% over 34bp)
TCGGCACCATGGAATTCTCGGGTGCCAAGGAACTCCAGTCACTACAGCATC19790.11283335442900848RNA PCR Primer, Index 43 (100% over 42bp)
CTGGGAATACCGGGTGCTGTAGGCTTTTGGAATTCTCGGGTGCCAAGGAAC19190.10941243413303046RNA PCR Primer, Index 1 (100% over 24bp)
TTAGTACTTGGATGGGAGACCGCCTGGAATTCTCGGGTGCCAAGGAACTCC19080.10878526541210118RNA PCR Primer, Index 1 (100% over 27bp)
GTTAGTACTTGGATGGGAGACCGCCTGGAATTCTCGGGTGCCAAGGAACTC18780.10707480526411216RNA PCR Primer, Index 1 (100% over 26bp)
CGTACGACTCTTAGCGGTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACT18280.10422403835079713RNA PCR Primer, Index 3 (100% over 34bp)
TCCCTGGTTCGATCCCGGGTTTCGGCACCATGGAATTCTCGGGTGCCAAGG17930.10222850151147661Illumina Small RNA Adapter 2 (100% over 21bp)
CCATGGAATTCTCGGGTGCCAAGGAACTCCAGTCACTACAGCATCTCGTAT17610.100404010686955RNA PCR Primer, Index 43 (100% over 48bp)

[FAIL]Adapter Content

Adapter graph