FastQCFastQC Report
Wed 27 Apr 2022
EGAF00005732245

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005732245
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1410673
Sequences flagged as poor quality0
Sequence length51
%GC55

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TCGTACGACTCTTAGCGGTGGAATTCTCGGGTGCCAAGGAACTCCAGTCAC836675.930998892018207RNA PCR Primer, Index 1 (100% over 33bp)
TGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCTAGCTATCTCGTATGCC201441.4279709046674884RNA PCR Primer, Index 38 (100% over 51bp)
GGAATACCGGGTGCTGTAGGCTTTGGAATTCTCGGGTGCCAAGGAACTCCA175201.241960397625814RNA PCR Primer, Index 1 (100% over 28bp)
GGAATACCGGGTGCTGTAGGCTTTTGGAATTCTCGGGTGCCAAGGAACTCC164971.1694418196137588RNA PCR Primer, Index 1 (100% over 27bp)
GAATACCGGGTGCTGTAGGCTTTGGAATTCTCGGGTGCCAAGGAACTCCAG157541.1167719237555407RNA PCR Primer, Index 1 (100% over 29bp)
AATACCGGGTGCTGTAGGCTTTGGAATTCTCGGGTGCCAAGGAACTCCAGT133910.9492632240072647RNA PCR Primer, Index 1 (100% over 30bp)
GTACGACTCTTAGCGGTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCT132540.9395515473819943RNA PCR Primer, Index 1 (97% over 35bp)
GAATACCGGGTGCTGTAGGCTTTTGGAATTCTCGGGTGCCAAGGAACTCCA129960.9212624045402443RNA PCR Primer, Index 1 (100% over 28bp)
AATACCGGGTGCTGTAGGCTTTTGGAATTCTCGGGTGCCAAGGAACTCCAG81440.5773130980744652RNA PCR Primer, Index 1 (100% over 29bp)
CCTGTCTGAGCGTCGCTTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACC79700.5649785598788664RNA PCR Primer, Index 2 (100% over 34bp)
CGCGACCTCAGATCAGACGTGGCGACCCGCTGATGGAATTCTCGGGTGCCA66270.46977577369099716No Hit
ACACAAGGGTTTGTTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCTAG65140.46176541267891286RNA PCR Primer, Index 3 (97% over 37bp)
TTGTACGACTCTTAGCGGTGGAATTCTCGGGTGCCAAGGAACTCCAGTCAC57720.40916640497124424RNA PCR Primer, Index 1 (100% over 33bp)
ATACCGGGTGCTGTAGGCTTTGGAATTCTCGGGTGCCAAGGAACTCCAGTC53990.3827251248163111RNA PCR Primer, Index 1 (100% over 31bp)
TCTGAGCGTCGCTTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCTAGC53960.38251246036466285RNA PCR Primer, Index 38 (100% over 38bp)
CCACGTTCCCGTGGTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCTAG47350.3356553928514971RNA PCR Primer, Index 3 (97% over 37bp)
TTTCGGCACCATGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCTAGCTA47290.33523006394820065RNA PCR Primer, Index 38 (100% over 40bp)
TTCGGGCGCCATGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCTAGCTA46060.32651082143062216RNA PCR Primer, Index 38 (100% over 40bp)
AATACCGGGTGCTGTAGGCTTATGGAATTCTCGGGTGCCAAGGAACTCCAG45290.3210524338383169RNA PCR Primer, Index 1 (100% over 29bp)
AATTCTCGGGTGCCAAGGAACTCCAGTCACCTAGCTATCTCGTATGCCGTC44640.31644470405260466RNA PCR Primer, Index 38 (100% over 51bp)
TTCGCGCTTTCCCCTGTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCT42100.2984391138130523RNA PCR Primer, Index 1 (97% over 35bp)
GTCTACGGCCATACCACCCTGAACGCGCCCGATGGAATTCTCGGGTGCCAA41630.2951073707372297No Hit
GAGACCGCCTGGGAATACCGGGTGCTGTAGGCTTTGGAATTCTCGGGTGCC38090.2700129654427355No Hit
GAATACCGGGTGCTGTAGGCTTGGAATTCTCGGGTGCCAAGGAACTCCAGT36690.26008862436581687RNA PCR Primer, Index 1 (100% over 30bp)
TACCGGGTGCTGTAGGCTTTGGAATTCTCGGGTGCCAAGGAACTCCAGTCA36600.2594506310108721RNA PCR Primer, Index 1 (100% over 32bp)
GGAATTCTCGGGTGCCAAGGAACTCCAGTCACCTAGCTATCTCGTATGCCG35580.2522200396548314RNA PCR Primer, Index 38 (100% over 51bp)
CCTGGGAATACCGGGTGCTGTAGGCTTTGGAATTCTCGGGTGCCAAGGAAC35340.2505187240416454RNA PCR Primer, Index 1 (100% over 24bp)
ATACCGGGTGCTGTAGGCTTTTGGAATTCTCGGGTGCCAAGGAACTCCAGT35250.24988073068670064RNA PCR Primer, Index 1 (100% over 30bp)
GAATACCGGGTGCTGTAGGCTTATGGAATTCTCGGGTGCCAAGGAACTCCA35020.248250303224064RNA PCR Primer, Index 1 (100% over 28bp)
GGTGCTGTAGGCTTTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCTAG34610.2453438890515378RNA PCR Primer, Index 3 (97% over 37bp)
GTCTGAGCGTCGCTTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCTAG34480.2444223430943954RNA PCR Primer, Index 3 (97% over 37bp)
GTCTACGGCCATACCACCCTGAACGCGCCCGTGGAATTCTCGGGTGCCAAG34420.24399701419109887No Hit
GGAATACCGGGTGCTGTAGGCTTGGAATTCTCGGGTGCCAAGGAACTCCAG33640.2384677384482442RNA PCR Primer, Index 1 (100% over 29bp)
CCGGGTGCTGTAGGCTTTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACC33450.23712086358780526RNA PCR Primer, Index 2 (100% over 34bp)
AATACCGGGTGCTGTAGGCTTGGAATTCTCGGGTGCCAAGGAACTCCAGTC33340.23634109393176167RNA PCR Primer, Index 1 (100% over 31bp)
GTTTCGGCACCATGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCTAGCT32320.22911050257572096RNA PCR Primer, Index 38 (100% over 39bp)
TCGTATGACTCTTAGCGGTGGAATTCTCGGGTGCCAAGGAACTCCAGTCAC32230.2284725092207762RNA PCR Primer, Index 1 (100% over 33bp)
CCCCACGTTGGGCGCCATGGAATTCTCGGGTGCCAAGGAACTCCAGTCACC31380.2224470164240756RNA PCR Primer, Index 2 (100% over 34bp)
TTCACAGTGGCTAAGTTCTGTGGAATTCTCGGGTGCCAAGGAACTCCAGTC30380.21535820136913375RNA PCR Primer, Index 1 (100% over 31bp)
AGACCGCCTGGGAATACCGGGTGCTGTAGGCTTTGGAATTCTCGGGTGCCA29450.20876560336803782No Hit
TCCTTCATTCCACCGGAGTCTGTGGAATTCTCGGGTGCCAAGGAACTCCAG29230.20720606405595063RNA PCR Primer, Index 1 (100% over 29bp)
CGCCTGTCTGAGCGTCGCTTGGAATTCTCGGGTGCCAAGGAACTCCAGTCA28920.2050085313889186RNA PCR Primer, Index 1 (100% over 32bp)
GTCTACGGCCATACCACCCTGAACGCGCCCGATCTGGAATTCTCGGGTGCC28630.2029527750229855No Hit
ACACAAGGGTTTGTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCTAGC28550.20238566981859016RNA PCR Primer, Index 38 (100% over 38bp)
GCCTGTCTGAGCGTCGCTTGGAATTCTCGGGTGCCAAGGAACTCCAGTCAC28450.20167678831309593RNA PCR Primer, Index 1 (100% over 33bp)
TCCCTGTTCGGGCGCCATGGAATTCTCGGGTGCCAAGGAACTCCAGTCACC28110.19926659119441573RNA PCR Primer, Index 2 (100% over 34bp)
ACCGGGTGCTGTAGGCTTTGGAATTCTCGGGTGCCAAGGAACTCCAGTCAC27820.19721083482848256RNA PCR Primer, Index 1 (100% over 33bp)
GTCTACGGCCATACCACCCTGAACGCGCCCGATTGGAATTCTCGGGTGCCA27370.19402086805375873No Hit
CGTTCGCGCTTTCCCCTGTGGAATTCTCGGGTGCCAAGGAACTCCAGTCAC26950.19104356573068315RNA PCR Primer, Index 1 (100% over 33bp)
CGACACAAGGGTTTGTTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCT26550.18820803970870642RNA PCR Primer, Index 1 (97% over 35bp)
GGGGCCCAAGTCCTTCTGATCGAGGCCTGGAATTCTCGGGTGCCAAGGAAC26190.18565606628892733RNA PCR Primer, Index 1 (100% over 24bp)
CCTGGGAATACCGGGTGCTGTAGGCTTTTGGAATTCTCGGGTGCCAAGGAA25890.18352942177244477RNA PCR Primer, Index 1 (100% over 23bp)
ATACCGGGTGCTGTAGGCTTATGGAATTCTCGGGTGCCAAGGAACTCCAGT25590.18140277725596224RNA PCR Primer, Index 1 (100% over 30bp)
CGCTTTCCCCTGTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCTAGCT24570.1741721858999215RNA PCR Primer, Index 38 (100% over 39bp)
GGAATACCGGGTGCTGTAGGCTTATGGAATTCTCGGGTGCCAAGGAACTCC24120.17098221912519768RNA PCR Primer, Index 1 (100% over 27bp)
GGTGCTGTAGGCTTTTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCTA23910.1694935679636599RNA PCR Primer, Index 2 (97% over 36bp)
TCCTTCTGATCGAGGCCTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACC23300.16516939078014536RNA PCR Primer, Index 2 (100% over 34bp)
TTTCCCGGCCAATGCACCATGGAATTCTCGGGTGCCAAGGAACTCCAGTCA23240.16474406187684884RNA PCR Primer, Index 1 (100% over 32bp)
CGGGTGCTGTAGGCTTTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCT23080.16360985146805815RNA PCR Primer, Index 1 (97% over 35bp)
TACCGGGTGCTGTAGGCTTTTGGAATTCTCGGGTGCCAAGGAACTCCAGTC22990.1629718581131134RNA PCR Primer, Index 1 (100% over 31bp)
TGTCTGAGCGTCGCTTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCTA22370.15857679277904943RNA PCR Primer, Index 2 (97% over 36bp)
CTACGCCTGTCTGAGCGTCGCTTGGAATTCTCGGGTGCCAAGGAACTCCAG20250.1435485048625727RNA PCR Primer, Index 1 (100% over 29bp)
TACTTGGATGGGAGACCGCCTGGAATTCTCGGGTGCCAAGGAACTCCAGTC20220.14333584041092443RNA PCR Primer, Index 1 (100% over 31bp)
CAAGGGTTTGTTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCTAGCTA20090.14241429445378198RNA PCR Primer, Index 38 (100% over 40bp)
GGGCTACGCCTGTCTGAGCGTCGCTTGGAATTCTCGGGTGCCAAGGAACTC19600.13894077507686048RNA PCR Primer, Index 1 (100% over 26bp)
CCGGGTGCTGTAGGCTTTTGGAATTCTCGGGTGCCAAGGAACTCCAGTCAC19500.13823189357136628RNA PCR Primer, Index 1 (100% over 33bp)
CTCGACACAAGGGTTTGTTGGAATTCTCGGGTGCCAAGGAACTCCAGTCAC19390.1374521239153227RNA PCR Primer, Index 1 (100% over 33bp)
GTCTACGGCCATACCACCCTGAACGCGCCCTGGAATTCTCGGGTGCCAAGG19080.1352545912482907Illumina Small RNA Adapter 2 (100% over 21bp)
TCGACACAAGGGTTTGTTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACC18850.1336241637856541RNA PCR Primer, Index 2 (100% over 34bp)
ATGGGAGACCGCCTGGGAATACCGGGTGCTGTAGGCTTTGGAATTCTCGGG18600.13185196002191862No Hit
AGTCACGGCACCATGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCTAGC18480.13100130221532558RNA PCR Primer, Index 38 (100% over 38bp)
CTCGGGTGCCAAGGAACTCCAGTCACCTAGCTATCTCGTATGCCGTCTTCT18200.12901643399994187RNA PCR Primer, Index 38 (100% over 51bp)
TCGTGGTTGTAGTCCGTGCGAGAATACCATGGAATTCTCGGGTGCCAAGGA17950.1272442302362064RNA PCR Primer, Index 1 (100% over 22bp)
ACCGGGTGCTGTAGGCTTTTGGAATTCTCGGGTGCCAAGGAACTCCAGTCA17790.1261100198274157RNA PCR Primer, Index 1 (100% over 32bp)
TAGCTTATCAGACTGATGTTGATGGAATTCTCGGGTGCCAAGGAACTCCAG17430.12355804640763664RNA PCR Primer, Index 1 (100% over 29bp)
TCACGGCACCATGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCTAGCTA17410.1234162701065378RNA PCR Primer, Index 38 (100% over 40bp)
TAGCTTATCAGACTGATGTTGACTGGAATTCTCGGGTGCCAAGGAACTCCA17360.1230618293537907RNA PCR Primer, Index 1 (100% over 28bp)
ATACCGGGTGCTGTAGGCTTGGAATTCTCGGGTGCCAAGGAACTCCAGTCA17360.1230618293537907RNA PCR Primer, Index 1 (100% over 32bp)
GCATTGGTGGTTCAGTGGTAGAATTCTCGCCTGCTGGAATTCTCGGGTGCC17260.12235294784829652No Hit
ACCGCCTGGGAATACCGGGTGCTGTAGGCTTTGGAATTCTCGGGTGCCAAG16790.11902120477247384No Hit
GCATTGGTGGTTCAGTGGTAGAATTCTCGCCTTGGAATTCTCGGGTGCCAA16500.11696544840654072No Hit
TACCCTGTAGAACCGAATTTGTTGGAATTCTCGGGTGCCAAGGAACTCCAG16470.11675278395489246RNA PCR Primer, Index 1 (100% over 29bp)
CACAAGGGTTTGTTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCTAGC16130.11434258683621222RNA PCR Primer, Index 38 (100% over 38bp)
GGGAATACCGGGTGCTGTAGGCTTTGGAATTCTCGGGTGCCAAGGAACTCC16000.11342104087906978RNA PCR Primer, Index 1 (100% over 27bp)
GTTTCCGTAGTGTAGTGGTTATCACGTTCGCCTTGGAATTCTCGGGTGCCA15090.10697021917907268No Hit
GCATTGGTGGTTCAGTGGTAGAATTCTCGCCTGTGGAATTCTCGGGTGCCA14790.10484357466259012No Hit
GTCTACGGCCATACCACCCTGAACGCGCCTGGAATTCTCGGGTGCCAAGGA14580.10335492350105234RNA PCR Primer, Index 1 (100% over 22bp)
TTAGTACTTGGATGGGAGACCGCCTGGAATTCTCGGGTGCCAAGGAACTCC14520.10292959459775582RNA PCR Primer, Index 1 (100% over 27bp)
TTGGGCGCCATGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCTAGCTAT14500.102787818296657RNA PCR Primer, Index 38 (100% over 41bp)
CGGGTGCTGTAGGCTTTTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACC14290.10129916713511919RNA PCR Primer, Index 2 (100% over 34bp)
TTTCCCGGCCAACGCACCATGGAATTCTCGGGTGCCAAGGAACTCCAGTCA14220.10080295008127325RNA PCR Primer, Index 1 (100% over 32bp)
TGGGGGGCCCAAGTCCTTCTGATCGAGGCCTGGAATTCTCGGGTGCCAAGG14170.10044850932852617Illumina Small RNA Adapter 2 (100% over 21bp)
CCGCCTGGGAATACCGGGTGCTGTAGGCTTTGGAATTCTCGGGTGCCAAGG14130.1001649567263285Illumina Small RNA Adapter 2 (100% over 21bp)

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)