FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005732248

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005732248
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences186072
Sequences flagged as poor quality0
Sequence length51
%GC54

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TCGTACGACTCTTAGCGGTGGAATTCTCGGGTGCCAAGGAACTCCAGTCAC71373.835612021153102RNA PCR Primer, Index 1 (100% over 33bp)
TGGAATTCTCGGGTGCCAAGGAACTCCAGTCACTCGAAGATCTCGTATGCC54822.9461713745216906RNA PCR Primer, Index 47 (100% over 51bp)
CCACGTTCCCGTGGTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACTCGA31321.6832193989423447RNA PCR Primer, Index 47 (100% over 37bp)
TCCTTCATTCCACCGGAGTCTGTGGAATTCTCGGGTGCCAAGGAACTCCAG29581.5897072101122147RNA PCR Primer, Index 1 (100% over 29bp)
GTACGACTCTTAGCGGTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACTC22071.186100004299411RNA PCR Primer, Index 5 (97% over 35bp)
TTCACAGTGGCTAAGTTCTGTGGAATTCTCGGGTGCCAAGGAACTCCAGTC17610.9464078421256288RNA PCR Primer, Index 1 (100% over 31bp)
GCATTGGTGGTTCAGTGGTAGAATTCTCGCCTGCTGGAATTCTCGGGTGCC16270.8743927081989767No Hit
TGAGATGAAGCACTGTAGCTTGGAATTCTCGGGTGCCAAGGAACTCCAGTC16040.8620319016294767RNA PCR Primer, Index 1 (100% over 31bp)
GGAATACCGGGTGCTGTAGGCTTTTGGAATTCTCGGGTGCCAAGGAACTCC15020.8072144116256073RNA PCR Primer, Index 1 (100% over 27bp)
AATTCTCGGGTGCCAAGGAACTCCAGTCACTCGAAGATCTCGTATGCCGTC13290.7142396491680639RNA PCR Primer, Index 47 (100% over 51bp)
TAGCTTATCAGACTGATGTTGACTGGAATTCTCGGGTGCCAAGGAACTCCA12520.6728578184788684RNA PCR Primer, Index 1 (100% over 28bp)
TAGCTTATCAGACTGATGTTGATGGAATTCTCGGGTGCCAAGGAACTCCAG12080.6492110580850424RNA PCR Primer, Index 1 (100% over 29bp)
TATTGCACTTGTCCCGGCCTGTTGGAATTCTCGGGTGCCAAGGAACTCCAG11390.6121286383765424RNA PCR Primer, Index 1 (100% over 29bp)
GGAATACCGGGTGCTGTAGGCTTTGGAATTCTCGGGTGCCAAGGAACTCCA11310.6078292273958468RNA PCR Primer, Index 1 (100% over 28bp)
GCATTGGTGGTTCAGTGGTAGAATTCTCGCCTTGGAATTCTCGGGTGCCAA10700.5750462186680425No Hit
TACCCTGTAGAACCGAATTTGTTGGAATTCTCGGGTGCCAAGGAACTCCAG10500.5642976912163034RNA PCR Primer, Index 1 (100% over 29bp)
ACCACGTTCCCGTGGTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACTCG10420.5599982802356077RNA PCR Primer, Index 10 (97% over 36bp)
TGAGGTAGTAGTTTGTGCTGTTTGGAATTCTCGGGTGCCAAGGAACTCCAG10010.5379637989595425RNA PCR Primer, Index 1 (100% over 29bp)
TGAGGTAGTAGGTTGTATAGTTTGGAATTCTCGGGTGCCAAGGAACTCCAG9850.5293649769981512RNA PCR Primer, Index 1 (100% over 29bp)
TCCTTCATTCCACCGGAGTCTGTTGGAATTCTCGGGTGCCAAGGAACTCCA9390.5046433638591513RNA PCR Primer, Index 1 (100% over 28bp)
AAGCTGCCAGTTGAAGAACTGTTGGAATTCTCGGGTGCCAAGGAACTCCAG9380.5041059374865643RNA PCR Primer, Index 1 (100% over 29bp)
CACCACGTTCCCGTGGTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACTC9370.5035685111139774RNA PCR Primer, Index 5 (97% over 35bp)
CCTGTCTGAGCGTCGCTTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACT8800.4729352078765209RNA PCR Primer, Index 3 (100% over 34bp)
GAATACCGGGTGCTGTAGGCTTTTGGAATTCTCGGGTGCCAAGGAACTCCA8330.447676168364934RNA PCR Primer, Index 1 (100% over 28bp)
TTCACAGTGGCTAAGTTCTGCTGGAATTCTCGGGTGCCAAGGAACTCCAGT8050.4326282299324993RNA PCR Primer, Index 1 (100% over 30bp)
GGAATTCTCGGGTGCCAAGGAACTCCAGTCACTCGAAGATCTCGTATGCCG7590.4079066167934993RNA PCR Primer, Index 47 (100% over 51bp)
CGCGACCTCAGATCAGACGTGGCGACCCGCTGATGGAATTCTCGGGTGCCA7380.3966206629691732No Hit
GGGGATTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACTCGAAGATCTCG7050.3788855926738037RNA PCR Primer, Index 47 (100% over 45bp)
TGAGGTAGTAGATTGTATAGTTTGGAATTCTCGGGTGCCAAGGAACTCCAG7020.3772733135560428RNA PCR Primer, Index 1 (100% over 29bp)
GAATACCGGGTGCTGTAGGCTTTGGAATTCTCGGGTGCCAAGGAACTCCAG6820.3665247861043037RNA PCR Primer, Index 1 (100% over 29bp)
GTCTGAGCGTCGCTTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACTCGA6790.3649125069865429RNA PCR Primer, Index 47 (100% over 37bp)
CAACGGAATCCCAAAAGCAGCTGTGGAATTCTCGGGTGCCAAGGAACTCCA6110.3283675136506299RNA PCR Primer, Index 1 (100% over 28bp)
GTTTCCGTAGTGTAGTGGTTATCACGTTCGCCTTGGAATTCTCGGGTGCCA5900.3170815598263038No Hit
TGAGATGAAGCACTGTAGCTCTGGAATTCTCGGGTGCCAAGGAACTCCAGT5360.2880605357066082RNA PCR Primer, Index 1 (100% over 30bp)
TTGTACGACTCTTAGCGGTGGAATTCTCGGGTGCCAAGGAACTCCAGTCAC5220.2805365664903908RNA PCR Primer, Index 1 (100% over 33bp)
TTCAAGTAATCCAGGATAGGCTTGGAATTCTCGGGTGCCAAGGAACTCCAG4960.26656348080312997RNA PCR Primer, Index 1 (100% over 29bp)
AACATTCAACGCTGTCGGTGAGTGGAATTCTCGGGTGCCAAGGAACTCCAG4840.2601143643320865RNA PCR Primer, Index 1 (100% over 29bp)
CATTGCACTTGTCTCGGTCTGATGGAATTCTCGGGTGCCAAGGAACTCCAG4740.25474010060621693RNA PCR Primer, Index 1 (100% over 29bp)
TGTCTGAGCGTCGCTTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACTCG4640.2493658368803474RNA PCR Primer, Index 10 (97% over 36bp)
GTTTGTGATGACTTACATGGAATTCTCGGGTGCCAAGGAACTCCAGTCACT4560.24506642589965175RNA PCR Primer, Index 3 (100% over 34bp)
CTCGGGTGCCAAGGAACTCCAGTCACTCGAAGATCTCGTATGCCGTCTTCT4390.2359301775656735RNA PCR Primer, Index 47 (100% over 51bp)
GGGGCTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACTCGAAGATCTCGT4370.2348553248204996RNA PCR Primer, Index 47 (100% over 46bp)
CGGGGCTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACTCGAAGATCTCG4240.2278687819768692RNA PCR Primer, Index 47 (100% over 45bp)
TGAGGTAGTAGGTTGTGTGGTTTGGAATTCTCGGGTGCCAAGGAACTCCAG4180.22464422374134743RNA PCR Primer, Index 1 (100% over 29bp)
GCATTGTGGTTCAGTGGTAGAATTCTCGCCTGCTGGAATTCTCGGGTGCCA4090.21980738638806485No Hit
GCCCGGCTAGCTCAGTCGGTAGAGCATGGGACTCTGGAATTCTCGGGTGCC4040.21712025452513004No Hit
AACATTCAACGCTGTCGGTGAGTTGGAATTCTCGGGTGCCAAGGAACTCCA4040.21712025452513004RNA PCR Primer, Index 1 (100% over 28bp)
TGAGATGAAGCACTGTAGCTCTTGGAATTCTCGGGTGCCAAGGAACTCCAG4040.21712025452513004RNA PCR Primer, Index 1 (100% over 29bp)
AATACCGGGTGCTGTAGGCTTTGGAATTCTCGGGTGCCAAGGAACTCCAGT4010.21550797540736918RNA PCR Primer, Index 1 (100% over 30bp)
TAGCAGCACGTAAATATTGGCGTGGAATTCTCGGGTGCCAAGGAACTCCAG3730.20046003697493445RNA PCR Primer, Index 1 (100% over 29bp)
TAGCTTATCAGACTGATGTTGTGGAATTCTCGGGTGCCAAGGAACTCCAGT3590.19293606775871705RNA PCR Primer, Index 1 (100% over 30bp)
TCCCTGGTGGTCTAGTGGTTAGGATTCGGCGCTTGGAATTCTCGGGTGCCA3560.1913237886409562No Hit
CGCGACCTCAGATCAGACGTGGCGACCCGCTGAATGGAATTCTCGGGTGCC3530.18971150952319532No Hit
CATTATTACTTTTGGTACGCGTGGAATTCTCGGGTGCCAAGGAACTCCAGT3530.18971150952319532RNA PCR Primer, Index 1 (100% over 30bp)
TCCCTGGTGGTCTAGTGGTTAGGATTCGGCGCTCTGGAATTCTCGGGTGCC3400.1827249666795649No Hit
GCATGGGTGGTTCAGTGGTAGAATTCTCGCCTTGGAATTCTCGGGTGCCAA3350.1800378348166301No Hit
GTTCCCGTGGTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACTCGAAGAT3340.17950040844404316RNA PCR Primer, Index 47 (100% over 41bp)
GCCCGGCTAGCTCAGTCGGTAGAGCATGAGACTCTGGAATTCTCGGGTGCC3260.17520099746334752No Hit
ATTCTCGGGTGCCAAGGAACTCCAGTCACTCGAAGATCTCGTATGCCGTCT3240.17412614471817361RNA PCR Primer, Index 47 (100% over 51bp)
CGTTCCCGTGGTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACTCGAAGA3210.17251386560041276RNA PCR Primer, Index 47 (100% over 40bp)
GCATGGGTGGTTCAGTGGTAGAATTCTCGCCTGCTGGAATTCTCGGGTGCC3210.17251386560041276No Hit
AATACCGGGTGCTGTAGGCTTTTGGAATTCTCGGGTGCCAAGGAACTCCAG3200.1719764392278258RNA PCR Primer, Index 1 (100% over 29bp)
TACCCTGTAGATCCGAATTTGTTGGAATTCTCGGGTGCCAAGGAACTCCAG3040.16337761726643452RNA PCR Primer, Index 1 (100% over 29bp)
CAAGGGTTTGTTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACTCGAAGA2990.16069048540349973RNA PCR Primer, Index 47 (100% over 40bp)
GTTTCCGTAGTGTAGTGGTTATCACGTTCGCCTCTGGAATTCTCGGGTGCC2880.1547787953050432No Hit
TCGTGGTTGTAGTCCGTGCGAGAATACCATGGAATTCTCGGGTGCCAAGGA2860.1537039425598693RNA PCR Primer, Index 1 (100% over 22bp)
GGGGCTACGCCTGTCTGAGCGTCGCTTGGAATTCTCGGGTGCCAAGGAACT2780.14940453157917366RNA PCR Primer, Index 1 (100% over 25bp)
GCATTGTGGTTCAGTGGTAGAATTCTCGCCTTGGAATTCTCGGGTGCCAAG2620.14080570961778235No Hit
CGCGACCTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACTCGAAGATCTC2610.1402682832451954RNA PCR Primer, Index 47 (100% over 44bp)
GCATTGGTGGTTCAGTGGTAGAATTCTCGCCTGTGGAATTCTCGGGTGCCA2560.13758115138226062No Hit
ACGTTCCCGTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACTCGAAGATC2540.13650629863708671RNA PCR Primer, Index 47 (100% over 42bp)
TGAGATGAAGCACTGTAGCTTTGGAATTCTCGGGTGCCAAGGAACTCCAGT2530.13596887226449977RNA PCR Primer, Index 1 (100% over 30bp)
TCCCGTGGTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACTCGAAGATCT2490.13381916677415195RNA PCR Primer, Index 47 (100% over 43bp)
ACACAAGGGTTTGTTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACTCGA2460.13220688765639108RNA PCR Primer, Index 47 (100% over 37bp)
AAGGGCTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACTCGAAGATCTCG2350.12629519755793456RNA PCR Primer, Index 47 (100% over 45bp)
CCACCACGTTCCCGTGGTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACT2340.12575777118534762RNA PCR Primer, Index 3 (100% over 34bp)
AGCAGCATTGTACAGGGCTATGATGGAATTCTCGGGTGCCAAGGAACTCCA2320.1246829184401737RNA PCR Primer, Index 1 (100% over 28bp)
CGCGACCTCAGATCAGACGTGGCGACCCGCTGAATTGGAATTCTCGGGTGC2310.12414549206758675No Hit
CGGGGCTACGCCTGTCTGAGCGTCGCTTGGAATTCTCGGGTGCCAAGGAAC2230.1198460810868911RNA PCR Primer, Index 1 (100% over 24bp)
GCCCGGCTAGCTCAGTCGGTAGAGCATGGGACTTGGAATTCTCGGGTGCCA2210.11877122834171719No Hit
TCTCGGGTGCCAAGGAACTCCAGTCACTCGAAGATCTCGTATGCCGTCTTC2180.11715894922395631RNA PCR Primer, Index 47 (100% over 51bp)
TAGCTTATCAGACTGATGTTGACTTGGAATTCTCGGGTGCCAAGGAACTCC2170.11662152285136936RNA PCR Primer, Index 1 (100% over 27bp)
GGAATACCGGGTGCTGTAGGCTTGGAATTCTCGGGTGCCAAGGAACTCCAG2140.1150092437336085RNA PCR Primer, Index 1 (100% over 29bp)
GGGGGTTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACTCGAAGATCTCG2130.11447181736102155RNA PCR Primer, Index 47 (100% over 45bp)
CGCCTGTCTGAGCGTCGCTTGGAATTCTCGGGTGCCAAGGAACTCCAGTCA2120.1139343909884346RNA PCR Primer, Index 1 (100% over 32bp)
GGGTGCTGTAGGCTTTTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACTC2100.11285953824326067RNA PCR Primer, Index 5 (97% over 35bp)
AGGGTTTGTTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACTCGAAGATC2090.11232211187067372RNA PCR Primer, Index 47 (100% over 42bp)
TCTGAGCGTCGCTTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACTCGAA2050.1101724063803259RNA PCR Primer, Index 47 (100% over 38bp)
TTTGGCAATGGTAGAACTCACACTTGGAATTCTCGGGTGCCAAGGAACTCC2030.109097553635152RNA PCR Primer, Index 1 (100% over 27bp)
GCCTGTCTGAGCGTCGCTTGGAATTCTCGGGTGCCAAGGAACTCCAGTCAC2020.10856012726256502RNA PCR Primer, Index 1 (100% over 33bp)
GGGTGCTGTAGGCTTTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACTCG2010.10802270088997806RNA PCR Primer, Index 10 (97% over 36bp)
TGAGGTAGTAGGTTGTATAGTTGGAATTCTCGGGTGCCAAGGAACTCCAGT2000.10748527451739111RNA PCR Primer, Index 1 (100% over 30bp)
CTCGTACGACTCTTAGCGGTGGAATTCTCGGGTGCCAAGGAACTCCAGTCA1990.10694784814480415RNA PCR Primer, Index 1 (100% over 32bp)
GGTGCTGTAGGCTTTTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACTCG1990.10694784814480415RNA PCR Primer, Index 10 (97% over 36bp)
TCGTATGACTCTTAGCGGTGGAATTCTCGGGTGCCAAGGAACTCCAGTCAC1960.1053355690270433RNA PCR Primer, Index 1 (100% over 33bp)
TAACACTGTCTGGTAAAGATGTGGAATTCTCGGGTGCCAAGGAACTCCAGT1910.10264843716410851RNA PCR Primer, Index 1 (100% over 30bp)
CACCCGTAGAACCGACCTTGCGTGGAATTCTCGGGTGCCAAGGAACTCCAG1900.10211101079152156RNA PCR Primer, Index 1 (100% over 29bp)

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TTATTGT502.1827873E-1145.00806822
ACACTTG253.874063E-545.00806820
AACCCGT253.874063E-545.0080681
TCAGTGC551.8189894E-1245.0080681
CCCGATC551.8189894E-1245.00806828
TCTTCAT253.874063E-545.0080682
CCGATTG253.874063E-545.00806829
GCCTGCC253.874063E-545.00806829
CTCGTAC551.8189894E-1245.0080681
TGGCTCA253.874063E-545.0080681
TCCTCGT207.011838E-445.0080641
TTGTTAA351.2028977E-745.00806425
TCCTTAA207.011838E-445.0080641
TTCACAG2850.045.0080641
CATTGCA650.045.0080641
TTTCCGT1300.045.0080642
GGACCCT207.011838E-445.00806425
ACCCGTA700.045.0080642
CAACACC302.1530068E-645.0080641
TAGTCGT351.2028977E-745.0080642
Produced by FastQC (version 0.11.5)