FastQCFastQC Report
Wed 27 Apr 2022
EGAF00005732249

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005732249
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1020054
Sequences flagged as poor quality0
Sequence length51
%GC53

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TGGAATTCTCGGGTGCCAAGGAACTCCAGTCACTATAATATCTCGTATGCC485494.759453911263521RNA PCR Primer, Index 44 (100% over 51bp)
GTTTCCGTAGTGTAGTGGTTATCACGTTCGCCTTGGAATTCTCGGGTGCCA149511.4657067174875056No Hit
GCATTGGTGGTTCAGTGGTAGAATTCTCGCCTTGGAATTCTCGGGTGCCAA127931.2541492901356204No Hit
TCGTACGACTCTTAGCGGTGGAATTCTCGGGTGCCAAGGAACTCCAGTCAC116001.1371946975356206RNA PCR Primer, Index 1 (100% over 33bp)
CCTGTCTGAGCGTCGCTTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACT80540.7895660425820594RNA PCR Primer, Index 3 (100% over 34bp)
GGAATACCGGGTGCTGTAGGCTTTTGGAATTCTCGGGTGCCAAGGAACTCC75600.7411372339111458RNA PCR Primer, Index 1 (100% over 27bp)
GGAATACCGGGTGCTGTAGGCTTTGGAATTCTCGGGTGCCAAGGAACTCCA68960.6760426408797966RNA PCR Primer, Index 1 (100% over 28bp)
TGAGATGAAGCACTGTAGCTTGGAATTCTCGGGTGCCAAGGAACTCCAGTC61640.604281734104273RNA PCR Primer, Index 1 (100% over 31bp)
CGCCTGTCTGAGCGTCGCTTGGAATTCTCGGGTGCCAAGGAACTCCAGTCA61430.6022230195656308RNA PCR Primer, Index 1 (100% over 32bp)
CCACGTTCCCGTGGTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACTATA54480.5340893717391432RNA PCR Primer, Index 43 (97% over 37bp)
GCATTGGTGGTTCAGTGGTAGAATTCTCGCCTGCTGGAATTCTCGGGTGCC50380.49389542122279795No Hit
GAATACCGGGTGCTGTAGGCTTTGGAATTCTCGGGTGCCAAGGAACTCCAG49940.48958192409421464RNA PCR Primer, Index 1 (100% over 29bp)
TACCCTGTAGAACCGAATTTGTTGGAATTCTCGGGTGCCAAGGAACTCCAG48260.47311220778507806RNA PCR Primer, Index 1 (100% over 29bp)
TCCTTCATTCCACCGGAGTCTGTGGAATTCTCGGGTGCCAAGGAACTCCAG47710.4677203363743488RNA PCR Primer, Index 1 (100% over 29bp)
GAATACCGGGTGCTGTAGGCTTTTGGAATTCTCGGGTGCCAAGGAACTCCA45870.44968207565481827RNA PCR Primer, Index 1 (100% over 28bp)
TTCACAGTGGCTAAGTTCTGTGGAATTCTCGGGTGCCAAGGAACTCCAGTC45320.44429020424408905RNA PCR Primer, Index 1 (100% over 31bp)
GTCTGAGCGTCGCTTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACTATA44780.4389963668590094RNA PCR Primer, Index 43 (97% over 37bp)
ATACCGGGTGCTGTAGGCTTTGGAATTCTCGGGTGCCAAGGAACTCCAGTC42490.4165465749852459RNA PCR Primer, Index 1 (100% over 31bp)
CCGGGTGCTGTAGGCTTTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACT40970.40164540308650326RNA PCR Primer, Index 3 (100% over 34bp)
TCTGAGCGTCGCTTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACTATAA40230.3943908851884312RNA PCR Primer, Index 44 (100% over 38bp)
GCCTGTCTGAGCGTCGCTTGGAATTCTCGGGTGCCAAGGAACTCCAGTCAC37930.37184305928901806RNA PCR Primer, Index 1 (100% over 33bp)
AATACCGGGTGCTGTAGGCTTTGGAATTCTCGGGTGCCAAGGAACTCCAGT37680.36939220864777744RNA PCR Primer, Index 1 (100% over 30bp)
GTACGACTCTTAGCGGTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACTA34160.33488423161911035RNA PCR Primer, Index 7 (97% over 35bp)
GTCTACGGCCATACCACCCTGAACGCGCCCGATGGAATTCTCGGGTGCCAA33820.33155107474702317No Hit
TACCGGGTGCTGTAGGCTTTGGAATTCTCGGGTGCCAAGGAACTCCAGTCA33420.3276297137210383RNA PCR Primer, Index 1 (100% over 32bp)
TAGCTTATCAGACTGATGTTGACTGGAATTCTCGGGTGCCAAGGAACTCCA33420.3276297137210383RNA PCR Primer, Index 1 (100% over 28bp)
TCCTGTACTGAGCTGCCCCGAGTGGAATTCTCGGGTGCCAAGGAACTCCAG32910.3226299784129076RNA PCR Primer, Index 1 (100% over 29bp)
TGAGGTAGTAGTTTGTGCTGTTTGGAATTCTCGGGTGCCAAGGAACTCCAG32560.3191987875151707RNA PCR Primer, Index 1 (100% over 29bp)
ATACCGGGTGCTGTAGGCTTTTGGAATTCTCGGGTGCCAAGGAACTCCAGT30680.30076839069304173RNA PCR Primer, Index 1 (100% over 30bp)
GCATTGGTGGTTCAGTGGTAGAATTCTCGCCTGTGGAATTCTCGGGTGCCA30640.30037625459044326No Hit
TAGCTTATCAGACTGATGTTGATGGAATTCTCGGGTGCCAAGGAACTCCAG30280.29684702966705684RNA PCR Primer, Index 1 (100% over 29bp)
TGTCTGAGCGTCGCTTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACTAT29790.2920433624102253RNA PCR Primer, Index 9 (97% over 36bp)
GGGGCTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACTATAATATCTCGT28720.28155372166571574RNA PCR Primer, Index 44 (100% over 46bp)
GCATTGTGGTTCAGTGGTAGAATTCTCGCCTTGGAATTCTCGGGTGCCAAG28470.2791028710244752No Hit
GTCTACGGCCATACCACCCTGAACGCGCCCGTGGAATTCTCGGGTGCCAAG27320.2678289580747686No Hit
CCGGGTGCTGTAGGCTTTTGGAATTCTCGGGTGCCAAGGAACTCCAGTCAC25280.24783001684224562RNA PCR Primer, Index 1 (100% over 33bp)
TCCCTGGTGGTCTAGTGGTTAGGATTCGGCGCTTGGAATTCTCGGGTGCCA24590.24106566907242166No Hit
GTCTACGGCCTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACTATAATAT24300.23822268232858262RNA PCR Primer, Index 44 (100% over 41bp)
AATACCGGGTGCTGTAGGCTTTTGGAATTCTCGGGTGCCAAGGAACTCCAG24130.23655610389253903RNA PCR Primer, Index 1 (100% over 29bp)
AGGGTTTGTTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACTATAATATC23490.2302819262509632RNA PCR Primer, Index 44 (100% over 42bp)
GTAGTCGTGGCCTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACTATAAT23180.2272428714558249RNA PCR Primer, Index 44 (100% over 39bp)
GGAATTCTCGGGTGCCAAGGAACTCCAGTCACTATAATATCTCGTATGCCG23030.22577236107108054RNA PCR Primer, Index 44 (100% over 51bp)
TACCGGGTGCTGTAGGCTTTTGGAATTCTCGGGTGCCAAGGAACTCCAGTC23020.22567432704543094RNA PCR Primer, Index 1 (100% over 31bp)
CGGGGCTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACTATAATATCTCG22750.22302740835289112RNA PCR Primer, Index 44 (100% over 45bp)
CGCGACCTCAGATCAGACGTGGCGACCCGCTGAATTGGAATTCTCGGGTGC22140.21704733278826416No Hit
GTCTACGGCCATACCACCCTGAACGCGCCCGATCTGGAATTCTCGGGTGCC21530.21106725722363717No Hit
CGCGACCTCAGATCAGACGTGGCGACCCGCTGATGGAATTCTCGGGTGCCA21140.20724393022330193No Hit
AATTCTCGGGTGCCAAGGAACTCCAGTCACTATAATATCTCGTATGCCGTC20610.2020481268638719RNA PCR Primer, Index 44 (100% over 51bp)
GTCTACGGCCATACCACCCTGAACGCGCCCGATTGGAATTCTCGGGTGCCA20540.20136188868432456No Hit
CTGTCTGAGCGTCGCTTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACTA20460.20057761647912756RNA PCR Primer, Index 7 (97% over 35bp)
ACCGGGTGCTGTAGGCTTTGGAATTCTCGGGTGCCAAGGAACTCCAGTCAC19750.19361720065800436RNA PCR Primer, Index 1 (100% over 33bp)
CGCGACCTCAGATCAGACGTGGCGACCCGCTGAATTTGGAATTCTCGGGTG19580.1919506222219608No Hit
GCATGGGTGGTTCAGTGGTAGAATTCTCGCCTTGGAATTCTCGGGTGCCAA19130.1875390910677278No Hit
GTTTCCGTAGTGTAGTGGTTATCACGTTCGCCTCTGGAATTCTCGGGTGCC18990.18616661470863305No Hit
CGGGTGCTGTAGGCTTTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACTA18940.18567644458038496RNA PCR Primer, Index 7 (97% over 35bp)
AAGCTGCCAGTTGAAGAACTGTTGGAATTCTCGGGTGCCAAGGAACTCCAG18820.18450003627258948RNA PCR Primer, Index 1 (100% over 29bp)
CTACGCCTGTCTGAGCGTCGCTTGGAATTCTCGGGTGCCAAGGAACTCCAG18550.18185311758004968RNA PCR Primer, Index 1 (100% over 29bp)
GTCTACGGCCATACCACCCTGAACGCGCCCTGGAATTCTCGGGTGCCAAGG16750.16420699296311764Illumina Small RNA Adapter 2 (100% over 21bp)
CGCGACCTCAGATCAGACGTGGCGACCCGCTGAATGGAATTCTCGGGTGCC16270.15950135973193574No Hit
GGGGCTACGCCTGTCTGAGCGTCGCTTGGAATTCTCGGGTGCCAAGGAACT16180.15861905350108915RNA PCR Primer, Index 1 (100% over 25bp)
CGGGGCTACGCCTGTCTGAGCGTCGCTTGGAATTCTCGGGTGCCAAGGAAC16090.15773674727024256RNA PCR Primer, Index 1 (100% over 24bp)
CAAGGGTTTGTTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACTATAATA15900.15587410078289973RNA PCR Primer, Index 44 (100% over 40bp)
ACCACGTTCCCGTGGTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACTAT15750.1544035903981554RNA PCR Primer, Index 9 (97% over 36bp)
GCATTGGTGGTTCAGTGGTAGAATTCTCGCCTGGAATTCTCGGGTGCCAAG15690.15381538624425767No Hit
TGAGGTAGTAGATTGTATAGTTTGGAATTCTCGGGTGCCAAGGAACTCCAG15630.15322718209035993RNA PCR Primer, Index 1 (100% over 29bp)
CGCGACCTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACTATAATATCTC15630.15322718209035993RNA PCR Primer, Index 44 (100% over 44bp)
GGGAATACCGGGTGCTGTAGGCTTTGGAATTCTCGGGTGCCAAGGAACTCC15570.1526389779364622RNA PCR Primer, Index 1 (100% over 27bp)
ATACCGGGTGCTGTAGGCTTGGAATTCTCGGGTGCCAAGGAACTCCAGTCA15350.1504822293721705RNA PCR Primer, Index 1 (100% over 32bp)
TCCTTCATTCCACCGGAGTCTGTTGGAATTCTCGGGTGCCAAGGAACTCCA15340.15038419534652087RNA PCR Primer, Index 1 (100% over 28bp)
CGACACAAGGGTTTGTTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACTA15210.14910975301307577RNA PCR Primer, Index 7 (97% over 35bp)
CTCGACACAAGGGTTTGTTGGAATTCTCGGGTGCCAAGGAACTCCAGTCAC14720.1443060857562443RNA PCR Primer, Index 1 (100% over 33bp)
GGGAATACCGGGTGCTGTAGGCTTTTGGAATTCTCGGGTGCCAAGGAACTC14630.14342377952539767RNA PCR Primer, Index 1 (100% over 26bp)
GGGTGCTGTAGGCTTTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACTAT14530.14244343926890146RNA PCR Primer, Index 9 (97% over 36bp)
TATTGCACTTGTCCCGGCCTGTTGGAATTCTCGGGTGCCAAGGAACTCCAG14270.13989455460201128RNA PCR Primer, Index 1 (100% over 29bp)
CGCGACCTCAGATCAGACGTGGCTGGAATTCTCGGGTGCCAAGGAACTCCA14250.13969848655071201RNA PCR Primer, Index 1 (100% over 28bp)
GTCTACGGCCATACCACCCTGAACGCGCCTGGAATTCTCGGGTGCCAAGGA14170.13891421434551504RNA PCR Primer, Index 1 (100% over 22bp)
GAATACCGGGTGCTGTAGGCTTGGAATTCTCGGGTGCCAAGGAACTCCAGT13940.1366594317555737RNA PCR Primer, Index 1 (100% over 30bp)
GGTGCTGTAGGCTTTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACTATA13850.13577712552472712RNA PCR Primer, Index 43 (97% over 37bp)
GGAATACCGGGTGCTGTAGGCTTGGAATTCTCGGGTGCCAAGGAACTCCAG13510.13244396865263994RNA PCR Primer, Index 1 (100% over 29bp)
CCGGGTGCTGTAGGCTTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACTA13410.13146362839614373RNA PCR Primer, Index 7 (97% over 35bp)
GTTTCCGTAGTGTAGTGGTCATCACGTTCGCCTTGGAATTCTCGGGTGCCA13270.13009115203704902No Hit
ACACAAGGGTTTGTTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACTATA13200.12940491385750166RNA PCR Primer, Index 43 (97% over 37bp)
ACCGGGTGCTGTAGGCTTTTGGAATTCTCGGGTGCCAAGGAACTCCAGTCA13110.12852260762665504RNA PCR Primer, Index 1 (100% over 32bp)
TCGACACAAGGGTTTGTTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACT12920.12665996113931224RNA PCR Primer, Index 3 (100% over 34bp)
AACATTCAACGCTGTCGGTGAGTGGAATTCTCGGGTGCCAAGGAACTCCAG12910.1265619271136626RNA PCR Primer, Index 1 (100% over 29bp)
TAGCAGCACGTAAATATTGGCGTGGAATTCTCGGGTGCCAAGGAACTCCAG12880.12626782503671374RNA PCR Primer, Index 1 (100% over 29bp)
TCGTGGTTGTAGTCCGTGCGAGAATACCATGGAATTCTCGGGTGCCAAGGA12730.1247973146519694RNA PCR Primer, Index 1 (100% over 22bp)
AAGGGTTTGTTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACTATAATAT12670.12420911049807166RNA PCR Primer, Index 44 (100% over 41bp)
CTGGGAATACCGGGTGCTGTAGGCTTTTGGAATTCTCGGGTGCCAAGGAAC12630.12381697439547316RNA PCR Primer, Index 1 (100% over 24bp)
CGGGTGCTGTAGGCTTTTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACT12510.12264056608767772RNA PCR Primer, Index 3 (100% over 34bp)
GGCTGGTCCGATGGTAGTGGGTTATCAGAACTTGGAATTCTCGGGTGCCAA12480.12234646401072884No Hit
TCCTGTACTGAGCTGCCCCGAGATGGAATTCTCGGGTGCCAAGGAACTCCA12430.12185629388248073RNA PCR Primer, Index 1 (100% over 28bp)
GTTTCCGTAGTGTAGTGGTTATCTGGAATTCTCGGGTGCCAAGGAACTCCA12350.12107202167728374RNA PCR Primer, Index 1 (100% over 28bp)
GGGGGCTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACTATAATATCTCG12320.12077791960033489RNA PCR Primer, Index 44 (100% over 45bp)
CGCGACCTCAGATCTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACTATA12220.11979757934383867RNA PCR Primer, Index 43 (97% over 37bp)
TGAGGTAGTAGGTTGTATAGTTTGGAATTCTCGGGTGCCAAGGAACTCCAG12190.11950347726688978RNA PCR Primer, Index 1 (100% over 29bp)
AATACCGGGTGCTGTAGGCTTGGAATTCTCGGGTGCCAAGGAACTCCAGTC12140.11901330713864168RNA PCR Primer, Index 1 (100% over 31bp)
TTCAAGTAATCCAGGATAGGCTTGGAATTCTCGGGTGCCAAGGAACTCCAG12000.11764083077954696RNA PCR Primer, Index 1 (100% over 29bp)
GGTGCTGTAGGCTTTTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACTAT11980.11744476272824772RNA PCR Primer, Index 9 (97% over 36bp)
GTTTTCGTAGTGTAGTGGTTATCACGTTCGCCTTGGAATTCTCGGGTGCCA11960.11724869467694848No Hit
CTGAGCGTCGCTTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACTATAAT11900.11666049052305075RNA PCR Primer, Index 44 (100% over 39bp)
GCATTGTGGTTCAGTGGTAGAATTCTCGCCTGCTGGAATTCTCGGGTGCCA11860.11626835442045225No Hit
CACCACGTTCCCGTGGTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACTA11810.11577818429220413RNA PCR Primer, Index 7 (97% over 35bp)
CCGGGGCTACGCCTGTCTGAGCGTCGCTTGGAATTCTCGGGTGCCAAGGAA11670.11440570793310942RNA PCR Primer, Index 1 (100% over 23bp)
GTCTACGGCCATACCACCCTGAACGCGCCCGATCTCGTCTGGAATTCTCGG11650.11420963988181018No Hit
GCCCGGCTAGCTCAGTCGGTAGAGCATGGGACTCTGGAATTCTCGGGTGCC11370.11146468716362073No Hit
CGACTCTTAGCGGTGGATCTGGAATTCTCGGGTGCCAAGGAACTCCAGTCA11110.10891580249673055RNA PCR Primer, Index 1 (100% over 32bp)
TCCCTGGTGGTCTAGTGGTTAGGATTCGGCGCTCTGGAATTCTCGGGTGCC11050.10832759834283281No Hit
GAGACCGCCTGGGAATACCGGGTGCTGTAGGCTTTGGAATTCTCGGGTGCC11030.10813153029153358No Hit
TACCCTGTAGATCCGAATTTGTTGGAATTCTCGGGTGCCAAGGAACTCCAG10970.10754332613763584RNA PCR Primer, Index 1 (100% over 29bp)
TGGGAATACCGGGTGCTGTAGGCTTTGGAATTCTCGGGTGCCAAGGAACTC10960.10744529211198622RNA PCR Primer, Index 1 (100% over 26bp)
ATCTAAAGGTCCCTGGTTCGATCCCGGGTTTCGGCACCATGGAATTCTCGG10890.10675905393243887No Hit
TGGGAATACCGGGTGCTGTAGGCTTTTGGAATTCTCGGGTGCCAAGGAACT10810.1059747817272419RNA PCR Primer, Index 1 (100% over 25bp)
TAGCTTATCAGACTGATGTTGACTTGGAATTCTCGGGTGCCAAGGAACTCC10750.10538657757334416RNA PCR Primer, Index 1 (100% over 27bp)
GTTTCCGTAGTGTAGTGGTTATCATGTTCGCCTTGGAATTCTCGGGTGCCA10690.10479837341944642No Hit
CATTGCACTTGTCTCGGTCTGATGGAATTCTCGGGTGCCAAGGAACTCCAG10670.10460230536814717RNA PCR Primer, Index 1 (100% over 29bp)
GGGGATTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACTATAATATCTCG10540.10332786303470208RNA PCR Primer, Index 44 (100% over 45bp)
TTGTACGACTCTTAGCGGTGGAATTCTCGGGTGCCAAGGAACTCCAGTCAC10290.10087701239346152RNA PCR Primer, Index 1 (100% over 33bp)
TACCGGGTGCTGTAGGCTTGGAATTCTCGGGTGCCAAGGAACTCCAGTCAC10250.10048487629086304RNA PCR Primer, Index 1 (100% over 33bp)
TGAGATGAAGCACTGTAGCTCTTGGAATTCTCGGGTGCCAAGGAACTCCAG10250.10048487629086304RNA PCR Primer, Index 1 (100% over 29bp)
TGAGATGAAGCACTGTAGCTCTGGAATTCTCGGGTGCCAAGGAACTCCAGT10240.10038684226521342RNA PCR Primer, Index 1 (100% over 30bp)

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)