FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005732252

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005732252
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5358729
Sequences flagged as poor quality0
Sequence length51
%GC53

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TGGAATTCTCGGGTGCCAAGGAACTCCAGTCACACTGATATCTCGTATGCC1654033.0866087835380367RNA PCR Primer, Index 25 (100% over 51bp)
CCTGTCTGAGCGTCGCTTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACA504270.9410253812051328RNA PCR Primer, Index 1 (100% over 34bp)
GAATACCGGGTGCTGTAGGCTTTGGAATTCTCGGGTGCCAAGGAACTCCAG503290.9391965893404948RNA PCR Primer, Index 1 (100% over 29bp)
AATACCGGGTGCTGTAGGCTTTGGAATTCTCGGGTGCCAAGGAACTCCAGT463690.8652984690959367RNA PCR Primer, Index 1 (100% over 30bp)
GGAATACCGGGTGCTGTAGGCTTTGGAATTCTCGGGTGCCAAGGAACTCCA459830.8580952684862398RNA PCR Primer, Index 1 (100% over 28bp)
GAATACCGGGTGCTGTAGGCTTTTGGAATTCTCGGGTGCCAAGGAACTCCA445050.830514101384862RNA PCR Primer, Index 1 (100% over 28bp)
GGAATACCGGGTGCTGTAGGCTTTTGGAATTCTCGGGTGCCAAGGAACTCC440140.8213514809201958RNA PCR Primer, Index 1 (100% over 27bp)
TAGCTTATCAGACTGATGTTGACTGGAATTCTCGGGTGCCAAGGAACTCCA432480.8070570465496576RNA PCR Primer, Index 1 (100% over 28bp)
TCTGAGCGTCGCTTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACACTGA422490.7884145662152351RNA PCR Primer, Index 25 (100% over 38bp)
AGGGTTTGTTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACACTGATATC388080.7242015783966683RNA PCR Primer, Index 25 (100% over 42bp)
TCCTTCATTCCACCGGAGTCTGTGGAATTCTCGGGTGCCAAGGAACTCCAG386460.7211784734775728RNA PCR Primer, Index 1 (100% over 29bp)
ACACAAGGGTTTGTTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACACTG382640.7140499174337795RNA PCR Primer, Index 5 (97% over 37bp)
GTTTCCGTAGTGTAGTGGTTATCACGTTCGCCTTGGAATTCTCGGGTGCCA362620.6766903121990308No Hit
AATACCGGGTGCTGTAGGCTTTTGGAATTCTCGGGTGCCAAGGAACTCCAG311080.5805107890322501RNA PCR Primer, Index 1 (100% over 29bp)
CGCCTGTCTGAGCGTCGCTTGGAATTCTCGGGTGCCAAGGAACTCCAGTCA307640.5740913563645409RNA PCR Primer, Index 1 (100% over 32bp)
CAAGGGTTTGTTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACACTGATA307180.5732329438566496RNA PCR Primer, Index 25 (100% over 40bp)
GGTGCTGTAGGCTTTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACACTG280700.5238182412284704RNA PCR Primer, Index 5 (97% over 37bp)
AAGGGTTTGTTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACACTGATAT279370.5213363094121759RNA PCR Primer, Index 25 (100% over 41bp)
GTCTGAGCGTCGCTTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACACTG273680.5107181199123897RNA PCR Primer, Index 5 (97% over 37bp)
TAGCTTATCAGACTGATGTTGATGGAATTCTCGGGTGCCAAGGAACTCCAG270850.5054370168747104RNA PCR Primer, Index 1 (100% over 29bp)
ATACCGGGTGCTGTAGGCTTTGGAATTCTCGGGTGCCAAGGAACTCCAGTC252930.4719962513499003RNA PCR Primer, Index 1 (100% over 31bp)
TCGTGGTTGTAGTCCGTGCGAGAATACCATGGAATTCTCGGGTGCCAAGGA229660.4285717751354845RNA PCR Primer, Index 1 (100% over 22bp)
TGTCTGAGCGTCGCTTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACACT224390.41873735357768604RNA PCR Primer, Index 8 (100% over 36bp)
GGTGCTGTAGGCTTTTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACACT216880.4047228363292863RNA PCR Primer, Index 8 (100% over 36bp)
TACCGGGTGCTGTAGGCTTTGGAATTCTCGGGTGCCAAGGAACTCCAGTCA215160.40151311999543177RNA PCR Primer, Index 1 (100% over 32bp)
CGCTTTCCCCTGTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACACTGAT215100.4014011531465763RNA PCR Primer, Index 25 (100% over 39bp)
CCGGGTGCTGTAGGCTTTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACA210630.39305962290684976RNA PCR Primer, Index 1 (100% over 34bp)
GCCTGTCTGAGCGTCGCTTGGAATTCTCGGGTGCCAAGGAACTCCAGTCAC202740.3783359822823658RNA PCR Primer, Index 1 (100% over 33bp)
TTCGGGCGCCATGGAATTCTCGGGTGCCAAGGAACTCCAGTCACACTGATA195750.36529184439071277RNA PCR Primer, Index 25 (100% over 40bp)
TAGCTTATCAGACTGATGTTGACTTGGAATTCTCGGGTGCCAAGGAACTCC195400.3646387044390563RNA PCR Primer, Index 1 (100% over 27bp)
ATACCGGGTGCTGTAGGCTTTTGGAATTCTCGGGTGCCAAGGAACTCCAGT189690.35398319265631834RNA PCR Primer, Index 1 (100% over 30bp)
CTACGCCTGTCTGAGCGTCGCTTGGAATTCTCGGGTGCCAAGGAACTCCAG187370.34965380783390987RNA PCR Primer, Index 1 (100% over 29bp)
CACAAGGGTTTGTTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACACTGA186610.3482355610817416RNA PCR Primer, Index 25 (100% over 38bp)
GCATTGGTGGTTCAGTGGTAGAATTCTCGCCTTGGAATTCTCGGGTGCCAA186490.3480116273840308No Hit
GTGCTGTAGGCTTTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACACTGA184810.3448765556160799RNA PCR Primer, Index 25 (100% over 38bp)
TTCGCGCTTTCCCCTGTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACAC180670.3371508430450579RNA PCR Primer, Index 5 (100% over 35bp)
CTGTCTGAGCGTCGCTTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACAC173910.3245359114073505RNA PCR Primer, Index 5 (100% over 35bp)
CGCGACCTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACACTGATATCTC171410.3198706260383759RNA PCR Primer, Index 25 (100% over 44bp)
GTGCTGTAGGCTTTTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACACTG171360.31977732033099643RNA PCR Primer, Index 5 (97% over 37bp)
AATACCGGGTGCTGTAGGCTTGGAATTCTCGGGTGCCAAGGAACTCCAGTC169360.31604509203581665RNA PCR Primer, Index 1 (100% over 31bp)
GAGACCGCCTGGGAATACCGGGTGCTGTAGGCTTTGGAATTCTCGGGTGCC167100.31182767406226364No Hit
ACCGGGTGCTGTAGGCTTTGGAATTCTCGGGTGCCAAGGAACTCCAGTCAC164620.3071997109762408RNA PCR Primer, Index 1 (100% over 33bp)
GTCTACGGCCATACCACCCTGAACGCGCCCGATGGAATTCTCGGGTGCCAA163170.30449384546223557No Hit
TACCGGGTGCTGTAGGCTTTTGGAATTCTCGGGTGCCAAGGAACTCCAGTC156470.2919908806733836RNA PCR Primer, Index 1 (100% over 31bp)
GAATACCGGGTGCTGTAGGCTTGGAATTCTCGGGTGCCAAGGAACTCCAGT153870.2871389838896499RNA PCR Primer, Index 1 (100% over 30bp)
CGGGGCTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACACTGATATCTCG149610.2791893376209172RNA PCR Primer, Index 25 (100% over 45bp)
CCGGGTGCTGTAGGCTTTTGGAATTCTCGGGTGCCAAGGAACTCCAGTCAC142560.26603323288040875RNA PCR Primer, Index 1 (100% over 33bp)
CCTGGGAATACCGGGTGCTGTAGGCTTTGGAATTCTCGGGTGCCAAGGAAC142070.26511883694808974RNA PCR Primer, Index 1 (100% over 24bp)
CGGGTGCTGTAGGCTTTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACAC142000.2649882089577585RNA PCR Primer, Index 5 (100% over 35bp)
TACCCTGTAGAACCGAATTTGTTGGAATTCTCGGGTGCCAAGGAACTCCAG140550.2622823434437532RNA PCR Primer, Index 1 (100% over 29bp)
TCCTTCATTCCACCGGAGTCTGTTGGAATTCTCGGGTGCCAAGGAACTCCA140550.2622823434437532RNA PCR Primer, Index 1 (100% over 28bp)
CTTTCCCCTGTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACACTGATAT135250.25239193846152697RNA PCR Primer, Index 25 (100% over 41bp)
TGAGATGAAGCACTGTAGCTTGGAATTCTCGGGTGCCAAGGAACTCCAGTC134760.25147754252920795RNA PCR Primer, Index 1 (100% over 31bp)
CTGAGCGTCGCTTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACACTGAT134530.2510483362752623RNA PCR Primer, Index 25 (100% over 39bp)
GTCTACGGCCATACCACCCTGAACGCGCCCGTGGAATTCTCGGGTGCCAAG133540.24920088326914835No Hit
TTCACAGTGGCTAAGTTCTGTGGAATTCTCGGGTGCCAAGGAACTCCAGTC132620.24748405825336567RNA PCR Primer, Index 1 (100% over 31bp)
CGTTCGCGCTTTCCCCTGTGGAATTCTCGGGTGCCAAGGAACTCCAGTCAC126590.2362313899433989RNA PCR Primer, Index 1 (100% over 33bp)
TCCCTGTTCGGGCGCCATGGAATTCTCGGGTGCCAAGGAACTCCAGTCACA125580.23434661465433315RNA PCR Primer, Index 1 (100% over 34bp)
CGACACAAGGGTTTGTTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACAC124670.23264845078002638RNA PCR Primer, Index 5 (100% over 35bp)
ACCGCTCTGACCATGGAATTCTCGGGTGCCAAGGAACTCCAGTCACACTGA124020.231435476584093RNA PCR Primer, Index 25 (100% over 38bp)
GGAATACCGGGTGCTGTAGGCTTGGAATTCTCGGGTGCCAAGGAACTCCAG120970.22574382843394397RNA PCR Primer, Index 1 (100% over 29bp)
ACCGGGTGCTGTAGGCTTTTGGAATTCTCGGGTGCCAAGGAACTCCAGTCA119590.22316859091026994RNA PCR Primer, Index 1 (100% over 32bp)
ACAAGGGTTTGTTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACACTGAT118820.22173168301662577RNA PCR Primer, Index 25 (100% over 39bp)
CCCCACGTTGGGCGCCATGGAATTCTCGGGTGCCAAGGAACTCCAGTCACA114760.214155259577411RNA PCR Primer, Index 1 (100% over 34bp)
CCTGGGAATACCGGGTGCTGTAGGCTTTTGGAATTCTCGGGTGCCAAGGAA112930.21074027068732157RNA PCR Primer, Index 1 (100% over 23bp)
TCGTACGACTCTTAGCGGTGGAATTCTCGGGTGCCAAGGAACTCCAGTCAC111780.20859423941759322RNA PCR Primer, Index 1 (100% over 33bp)
TTTCGGCACCATGGAATTCTCGGGTGCCAAGGAACTCCAGTCACACTGATA111310.20771716576822602RNA PCR Primer, Index 25 (100% over 40bp)
TGAGCGTCGCTTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACACTGATA108520.20251070729645032RNA PCR Primer, Index 25 (100% over 40bp)
CCGCCTGGGAATACCGGGTGCTGTAGGCTTTGGAATTCTCGGGTGCCAAGG105430.1967444145803977Illumina Small RNA Adapter 2 (100% over 21bp)
CAACACCAGTCGATGGGCTGTTGGAATTCTCGGGTGCCAAGGAACTCCAGT103460.1930681697096457RNA PCR Primer, Index 1 (100% over 30bp)
AAGGACCATGGAATTCTCGGGTGCCAAGGAACTCCAGTCACACTGATATCT101280.18900004086789984RNA PCR Primer, Index 25 (100% over 43bp)
GGGGCTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACACTGATATCTCGT100260.18709660443735818RNA PCR Primer, Index 25 (100% over 46bp)
TGCTGTAGGCTTTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACACTGAT100180.186947315305551RNA PCR Primer, Index 25 (100% over 39bp)
CGGGTGCTGTAGGCTTTTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACA99750.18614488622208736RNA PCR Primer, Index 1 (100% over 34bp)
TCGACACAAGGGTTTGTTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACA99190.18509986229943703RNA PCR Primer, Index 1 (100% over 34bp)
CTCGACACAAGGGTTTGTTGGAATTCTCGGGTGCCAAGGAACTCCAGTCAC99100.18493191202615394RNA PCR Primer, Index 1 (100% over 33bp)
GGTGCTGTAGGCTTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACACTGA98890.1845400280551601RNA PCR Primer, Index 25 (100% over 38bp)
AGACCGCCTGGGAATACCGGGTGCTGTAGGCTTTGGAATTCTCGGGTGCCA97470.1818901459655825No Hit
GTAGTCGTGGCCTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACACTGAT96430.17994938725208906RNA PCR Primer, Index 25 (100% over 39bp)
TGAGGTAGTAGATTGTATAGTTTGGAATTCTCGGGTGCCAAGGAACTCCAG95780.17873641305615567RNA PCR Primer, Index 1 (100% over 29bp)
ACCGCCTGGGAATACCGGGTGCTGTAGGCTTTGGAATTCTCGGGTGCCAAG92140.17194375755892863No Hit
ATACCGGGTGCTGTAGGCTTGGAATTCTCGGGTGCCAAGGAACTCCAGTCA90060.16806224013194174RNA PCR Primer, Index 1 (100% over 32bp)
TAGCTTATCAGACTGATGTTGTGGAATTCTCGGGTGCCAAGGAACTCCAGT88650.16543101918384004RNA PCR Primer, Index 1 (100% over 30bp)
TATTGCACTTGTCCCGGCCTGTTGGAATTCTCGGGTGCCAAGGAACTCCAG85970.16042983326829927RNA PCR Primer, Index 1 (100% over 29bp)
GGGTGCTGTAGGCTTTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACACT84240.15720145579296882RNA PCR Primer, Index 8 (100% over 36bp)
TACGCCTGTCTGAGCGTCGCTTGGAATTCTCGGGTGCCAAGGAACTCCAGT80490.15020352773950688RNA PCR Primer, Index 1 (100% over 30bp)
TGGGAATACCGGGTGCTGTAGGCTTTGGAATTCTCGGGTGCCAAGGAACTC80460.15014754431507918RNA PCR Primer, Index 1 (100% over 26bp)
GCATGGGTGGTTCAGTGGTAGAATTCTCGCCTTGGAATTCTCGGGTGCCAA79650.14863599185553142No Hit
GTGCTGTAGGCTTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACACTGAT78000.14555690351200817RNA PCR Primer, Index 25 (100% over 39bp)
CTCGAAGGACCATGGAATTCTCGGGTGCCAAGGAACTCCAGTCACACTGAT76210.14221655918782233RNA PCR Primer, Index 25 (100% over 39bp)
CTGTAGGCTTTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACACTGATAT75440.14077965129417816RNA PCR Primer, Index 25 (100% over 41bp)
CCGGGTGCTGTAGGCTTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACAC74490.13900684285396778RNA PCR Primer, Index 5 (100% over 35bp)
GTCTACGGCCATACCACCCTGAACGCGCCCTGGAATTCTCGGGTGCCAAGG74440.1389135371465883Illumina Small RNA Adapter 2 (100% over 21bp)
GGGAATACCGGGTGCTGTAGGCTTTGGAATTCTCGGGTGCCAAGGAACTCC73880.137868513223938RNA PCR Primer, Index 1 (100% over 27bp)
AATACCGGGTGCTGTAGGCTTATGGAATTCTCGGGTGCCAAGGAACTCCAG72460.13521863113436042RNA PCR Primer, Index 1 (100% over 29bp)
CCTGTCTGAGCGTCGCTTTGGAATTCTCGGGTGCCAAGGAACTCCAGTCAC72290.13490139172927013RNA PCR Primer, Index 1 (100% over 33bp)
GTCTACGGCCATACCACCCTGAACGCGCCCGATTGGAATTCTCGGGTGCCA72100.13454683004122805No Hit
ACACAAGGGTTTGTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACACTGA71060.13260607132773464RNA PCR Primer, Index 25 (100% over 38bp)
TGGGAATACCGGGTGCTGTAGGCTTTTGGAATTCTCGGGTGCCAAGGAACT70440.13144908055622892RNA PCR Primer, Index 1 (100% over 25bp)
TACCGGGTGCTGTAGGCTTGGAATTCTCGGGTGCCAAGGAACTCCAGTCAC70100.13081460174604836RNA PCR Primer, Index 1 (100% over 33bp)
GTCTACGGCCTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACACTGATAT69770.1301987840773437RNA PCR Primer, Index 25 (100% over 41bp)
GTCTACGGCCATACCACCCTGAACGCGCCCGATCTGGAATTCTCGGGTGCC67190.1253842095765619No Hit
GCATTGGTGGTTCAGTGGTAGAATTCTCGCCTGCTGGAATTCTCGGGTGCC66240.12361140113635155No Hit
GGGAATACCGGGTGCTGTAGGCTTTTGGAATTCTCGGGTGCCAAGGAACTC66030.12321951716535767RNA PCR Primer, Index 1 (100% over 26bp)
GAATACCGGGTGCTGTAGGCTTATGGAATTCTCGGGTGCCAAGGAACTCCA65050.12139072530071963RNA PCR Primer, Index 1 (100% over 28bp)
TGCTGTAGGCTTTTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACACTGA64970.12124143616891243RNA PCR Primer, Index 25 (100% over 38bp)
ATTTCAACTTAACTTGACCGCTCTGACCATGGAATTCTCGGGTGCCAAGGA64690.12071892420758729RNA PCR Primer, Index 1 (100% over 22bp)
ATACCGGGTGCTGTAGGCTTATGGAATTCTCGGGTGCCAAGGAACTCCAGT62950.11747188559078094RNA PCR Primer, Index 1 (100% over 30bp)
CTGGGAATACCGGGTGCTGTAGGCTTTGGAATTCTCGGGTGCCAAGGAACT62380.11640820052665472RNA PCR Primer, Index 1 (100% over 25bp)
GCTTCTGTAGTGTAGTGGTTATCACGTTCGCCTTGGAATTCTCGGGTGCCA61910.1155311268772875No Hit
GCATTGTGGTTCAGTGGTAGAATTCTCGCCTTGGAATTCTCGGGTGCCAAG61350.1144861029546372No Hit
GGGTGCTGTAGGCTTTTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACAC59650.11131370890373445RNA PCR Primer, Index 5 (100% over 35bp)
ACCGGGTGCTGTAGGCTTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACA59250.1105672632446985RNA PCR Primer, Index 1 (100% over 34bp)
CTGGGAATACCGGGTGCTGTAGGCTTTTGGAATTCTCGGGTGCCAAGGAAC57470.10724558006198859RNA PCR Primer, Index 1 (100% over 24bp)
CACGTTGGGCGCCATGGAATTCTCGGGTGCCAAGGAACTCCAGTCACACTG57470.10724558006198859RNA PCR Primer, Index 5 (97% over 37bp)
CTCTGACCATGGAATTCTCGGGTGCCAAGGAACTCCAGTCACACTGATATC57000.10636850641262134RNA PCR Primer, Index 25 (100% over 42bp)

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TACCTTA551.8189894E-1245.0155331
AACTAGA207.0236524E-445.0155331
TCCCACA650.045.0155331
GGGGGTA453.8380676E-1045.015531
AATAGCG207.027852E-445.0100672
GACCTTA302.1675569E-644.9983144
TTAATGA207.036904E-444.998313
ATAGCGG253.8935883E-544.998313
GCACATA207.036904E-444.998316
GTAGTAA253.8935883E-544.998315
CAACGCT4750.044.997897
AGACTTA551.8189894E-1244.9974710
AGTTTCG207.037551E-444.9974714
CCATACG207.037551E-444.997479
CAAATAC253.8940176E-544.9974712
GGCATAG207.037551E-444.9974713
ACATAAT207.037551E-444.997478
GTAACGA253.8940176E-544.9974713
CCACCGA700.044.9974710
TCAAATA253.8940176E-544.9974711
Produced by FastQC (version 0.11.5)