FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005732254

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005732254
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3137705
Sequences flagged as poor quality0
Sequence length51
%GC53

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TGGAATTCTCGGGTGCCAAGGAACTCCAGTCACAGTTCCATCTCGTATGCC139797744.554124750414715RNA PCR Primer, Index 14 (100% over 51bp)
TCGTACGACTCTTAGCGGTGGAATTCTCGGGTGCCAAGGAACTCCAGTCAC424451.3527402990402222RNA PCR Primer, Index 1 (100% over 33bp)
TCCTTCATTCCACCGGAGTCTGTGGAATTCTCGGGTGCCAAGGAACTCCAG335731.069985865465364RNA PCR Primer, Index 1 (100% over 29bp)
GGAATACCGGGTGCTGTAGGCTTTTGGAATTCTCGGGTGCCAAGGAACTCC169010.5386420966916903RNA PCR Primer, Index 1 (100% over 27bp)
GGAATTCTCGGGTGCCAAGGAACTCCAGTCACAGTTCCATCTCGTATGCCG165200.5264994637800559RNA PCR Primer, Index 14 (100% over 51bp)
GGAATACCGGGTGCTGTAGGCTTTGGAATTCTCGGGTGCCAAGGAACTCCA145810.4647027046838374RNA PCR Primer, Index 1 (100% over 28bp)
GTACGACTCTTAGCGGTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACAG142180.45313373946881563RNA PCR Primer, Index 2 (97% over 35bp)
TAGCTTATCAGACTGATGTTGACTGGAATTCTCGGGTGCCAAGGAACTCCA135310.43123875571476605RNA PCR Primer, Index 1 (100% over 28bp)
TACCCTGTAGAACCGAATTTGTTGGAATTCTCGGGTGCCAAGGAACTCCAG132100.4210083484585071RNA PCR Primer, Index 1 (100% over 29bp)
CCACGTTCCCGTGGTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACAGTT131200.4181400099754438RNA PCR Primer, Index 8 (97% over 37bp)
CCTGTCTGAGCGTCGCTTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACA127910.4076546392984682RNA PCR Primer, Index 1 (100% over 34bp)
GAATACCGGGTGCTGTAGGCTTTGGAATTCTCGGGTGCCAAGGAACTCCAG116470.3711948701359752RNA PCR Primer, Index 1 (100% over 29bp)
GAATACCGGGTGCTGTAGGCTTTTGGAATTCTCGGGTGCCAAGGAACTCCA115290.3674341596804033RNA PCR Primer, Index 1 (100% over 28bp)
ACACAAGGGTTTGTTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACAGTT94940.3025778395355841RNA PCR Primer, Index 8 (97% over 37bp)
GGGAATACCGGGTGCTGTAGGCTTTTGGAATTCTCGGGTGCCAAGGAACTC86290.2750099196705873RNA PCR Primer, Index 1 (100% over 26bp)
TCGTGGTTGTAGTCCGTGCGAGAATACCATGGAATTCTCGGGTGCCAAGGA86240.2748505675326393RNA PCR Primer, Index 1 (100% over 22bp)
TTCACAGTGGCTAAGTTCTGTGGAATTCTCGGGTGCCAAGGAACTCCAGTC84170.26825338902159385RNA PCR Primer, Index 1 (100% over 31bp)
GGGAATACCGGGTGCTGTAGGCTTTGGAATTCTCGGGTGCCAAGGAACTCC83620.26650051550416626RNA PCR Primer, Index 1 (100% over 27bp)
TAGCTTATCAGACTGATGTTGATGGAATTCTCGGGTGCCAAGGAACTCCAG83030.26462016027638036RNA PCR Primer, Index 1 (100% over 29bp)
GCATTGGTGGTTCAGTGGTAGAATTCTCGCCTTGGAATTCTCGGGTGCCAA80800.25751305492390136No Hit
TAGCTTATCAGACTGATGTTGACTTGGAATTCTCGGGTGCCAAGGAACTCC77950.24842998306086775RNA PCR Primer, Index 1 (100% over 27bp)
TGGGAATACCGGGTGCTGTAGGCTTTTGGAATTCTCGGGTGCCAAGGAACT73940.23564994159744143RNA PCR Primer, Index 1 (100% over 25bp)
CGCCTGTCTGAGCGTCGCTTGGAATTCTCGGGTGCCAAGGAACTCCAGTCA73270.23351462294893877RNA PCR Primer, Index 1 (100% over 32bp)
GTCTACGGCCATACCACCCTGAACGCGCCCGATGGAATTCTCGGGTGCCAA71840.22895715180362716No Hit
TGAGATGAAGCACTGTAGCTTGGAATTCTCGGGTGCCAAGGAACTCCAGTC69380.2211170266165876RNA PCR Primer, Index 1 (100% over 31bp)
TGGGAATACCGGGTGCTGTAGGCTTTGGAATTCTCGGGTGCCAAGGAACTC68650.21879048540254742RNA PCR Primer, Index 1 (100% over 26bp)
CCGGGTGCTGTAGGCTTTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACA66870.2131175492916001RNA PCR Primer, Index 1 (100% over 34bp)
GCATTGGTGGTTCAGTGGTAGAATTCTCGCCTGCTGGAATTCTCGGGTGCC66380.21155589833971009No Hit
AATACCGGGTGCTGTAGGCTTTGGAATTCTCGGGTGCCAAGGAACTCCAGT65860.2098986361050513RNA PCR Primer, Index 1 (100% over 30bp)
TCCTTCATTCCACCGGAGTCTGTTGGAATTCTCGGGTGCCAAGGAACTCCA63630.20279153075257236RNA PCR Primer, Index 1 (100% over 28bp)
TCTGAGCGTCGCTTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACAGTTC63260.20161232493175743RNA PCR Primer, Index 14 (100% over 38bp)
TGGAATTCTTGGGTGCCAAGGAACTCCAGTCACAGTTCCATCTCGTATGCC60560.19300730948256767RNA PCR Primer, Index 14 (98% over 51bp)
CCTGGGAATACCGGGTGCTGTAGGCTTTGGAATTCTCGGGTGCCAAGGAAC57810.18424294189542995RNA PCR Primer, Index 1 (100% over 24bp)
CGACACAAGGGTTTGTTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACAG51180.1631128484035306RNA PCR Primer, Index 2 (97% over 35bp)
CCTGGGAATACCGGGTGCTGTAGGCTTTTGGAATTCTCGGGTGCCAAGGAA50250.16014889863769857RNA PCR Primer, Index 1 (100% over 23bp)
CGCGACCTCAGATCAGACGTGGCTGGAATTCTCGGGTGCCAAGGAACTCCA50090.1596389717962651RNA PCR Primer, Index 1 (100% over 28bp)
CTGGGAATACCGGGTGCTGTAGGCTTTTGGAATTCTCGGGTGCCAAGGAAC49570.15798170956160634RNA PCR Primer, Index 1 (100% over 24bp)
CAAGGGTTTGTTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACAGTTCCA47950.15281870029209246RNA PCR Primer, Index 14 (100% over 40bp)
GAATACCGGGTGCTGTAGGCTTGGAATTCTCGGGTGCCAAGGAACTCCAGT47410.15109769720225452RNA PCR Primer, Index 1 (100% over 30bp)
CCGCCTGGGAATACCGGGTGCTGTAGGCTTTGGAATTCTCGGGTGCCAAGG46350.1477194318777578Illumina Small RNA Adapter 2 (100% over 21bp)
CTGTCTGAGCGTCGCTTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACAG46250.14740072760186185RNA PCR Primer, Index 2 (97% over 35bp)
GTCTGAGCGTCGCTTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACAGTT45630.14542476109130717RNA PCR Primer, Index 8 (97% over 37bp)
TGGAATTCTCGGGTGCCAAGGAACTCCAGTCACAGTTCCATTTCGTATGCC45430.14478735253951536RNA PCR Primer, Index 14 (98% over 51bp)
GTCTACGGCCATACCACCCTTGGAATTCTCGGGTGCCAAGGAACTCCAGTC45270.14427742569808188RNA PCR Primer, Index 1 (100% over 31bp)
GTTTCCGTAGTGTAGTGGTTATCACGTTCGCCTTGGAATTCTCGGGTGCCA45020.14348066500834208No Hit
GTCTACGGCCATACCACCCTGAACGCGCCCGTGGAATTCTCGGGTGCCAAG44860.1429707381669086No Hit
CCGGGTGCTGTAGGCTTTTGGAATTCTCGGGTGCCAAGGAACTCCAGTCAC44550.1419827549116313RNA PCR Primer, Index 1 (100% over 33bp)
GGAATACCGGGTGCTGTAGGCTTGGAATTCTCGGGTGCCAAGGAACTCCAG42660.13595924409719845RNA PCR Primer, Index 1 (100% over 29bp)
AATACCGGGTGCTGTAGGCTTTTGGAATTCTCGGGTGCCAAGGAACTCCAG42570.13567241024889212RNA PCR Primer, Index 1 (100% over 29bp)
CTGGGAATACCGGGTGCTGTAGGCTTTGGAATTCTCGGGTGCCAAGGAACT40260.12831034147569642RNA PCR Primer, Index 1 (100% over 25bp)
ACCACGTTCCCGTGGTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACAGT39460.12576070726852906RNA PCR Primer, Index 8 (97% over 36bp)
CCCCACGTTGGGCGCCATGGAATTCTCGGGTGCCAAGGAACTCCAGTCACA39140.12474085358566214RNA PCR Primer, Index 1 (100% over 34bp)
CGGGTGCTGTAGGCTTTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACAG38920.12403970417869112RNA PCR Primer, Index 2 (97% over 35bp)
CACCACGTTCCCGTGGTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACAG36800.11728317352969765RNA PCR Primer, Index 2 (97% over 35bp)
GCCTGTCTGAGCGTCGCTTGGAATTCTCGGGTGCCAAGGAACTCCAGTCAC36160.1152434661639638RNA PCR Primer, Index 1 (100% over 33bp)
GAGACCGCCTGGGAATACCGGGTGCTGTAGGCTTTGGAATTCTCGGGTGCC35850.11425548290868645No Hit
TCGACACAAGGGTTTGTTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACA35280.11243886853607972RNA PCR Primer, Index 1 (100% over 34bp)
TGGAATTCTCGGGTGCCAAGGAACTCTAGTCACAGTTCCATCTCGTATGCC34740.11071786544624175RNA PCR Primer, Index 14 (98% over 51bp)
GTCTACGGCCATACCACCCTGAACGCGCCCGATTGGAATTCTCGGGTGCCA34500.10995297518409156No Hit
TGAGGTAGTAGGTTGTATAGTTTGGAATTCTCGGGTGCCAAGGAACTCCAG34240.10912434406676218RNA PCR Primer, Index 1 (100% over 29bp)
CGCGACCTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACAGTTCCATCTC33510.10679780285272197RNA PCR Primer, Index 14 (100% over 44bp)
TCCCTGTTCGGGCGCCATGGAATTCTCGGGTGCCAAGGAACTCCAGTCACA33030.10526802232842156RNA PCR Primer, Index 1 (100% over 34bp)
AATACCGGGTGCTGTAGGCTTGGAATTCTCGGGTGCCAAGGAACTCCAGTC32750.10437565035591301RNA PCR Primer, Index 1 (100% over 31bp)
TGTCTGAGCGTCGCTTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACAGT32620.10396133479724831RNA PCR Primer, Index 8 (97% over 36bp)
CTACGCCTGTCTGAGCGTCGCTTGGAATTCTCGGGTGCCAAGGAACTCCAG31720.10109299631418506RNA PCR Primer, Index 1 (100% over 29bp)
GGTGCTGTAGGCTTTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACAGTT31640.10083803289346832RNA PCR Primer, Index 8 (97% over 37bp)

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTGTACC207.0169603E-445.0236631
CAACGGA1750.045.0236631
TGTGCAA207.0169603E-445.0236631
GAAAAAG302.1588803E-645.0236631
TACTAGA207.0169603E-445.0236631
TGAGGTA13100.045.0236631
TCCCATA351.2078272E-745.0236631
AACACCC351.2101009E-745.01292
AAAAAGT253.8857732E-545.0128972
TTAAACC207.0252316E-445.0128942
ACCCGTA1150.045.0128942
GGCACGC207.032407E-445.00356722
CACGCCA207.032407E-445.00356724
AGGCACG207.032407E-445.00356721
ACGCGCT207.032407E-445.00356723
TCGCCGT207.0340635E-445.0014228
GGTTATT253.8916296E-545.0014220
CCTTAGA253.8927283E-544.9992686
TTAATGA207.03572E-444.9992683
ATTACTT800.044.9992685
Produced by FastQC (version 0.11.5)