FastQCFastQC Report
Wed 27 Apr 2022
EGAF00005732256

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005732256
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences773792
Sequences flagged as poor quality0
Sequence length51
%GC55

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGAATACCGGGTGCTGTAGGCTTTTGGAATTCTCGGGTGCCAAGGAACTCC280843.6293991150076503RNA PCR Primer, Index 1 (100% over 27bp)
GGAATACCGGGTGCTGTAGGCTTTGGAATTCTCGGGTGCCAAGGAACTCCA227912.9453651627310697RNA PCR Primer, Index 1 (100% over 28bp)
GAATACCGGGTGCTGTAGGCTTTTGGAATTCTCGGGTGCCAAGGAACTCCA167062.1589781233199616RNA PCR Primer, Index 1 (100% over 28bp)
GAATACCGGGTGCTGTAGGCTTTGGAATTCTCGGGTGCCAAGGAACTCCAG145001.8738885902154583RNA PCR Primer, Index 1 (100% over 29bp)
TCGTACGACTCTTAGCGGTGGAATTCTCGGGTGCCAAGGAACTCCAGTCAC110891.4330724535792565RNA PCR Primer, Index 1 (100% over 33bp)
TACCCTGTAGAACCGAATTTGTTGGAATTCTCGGGTGCCAAGGAACTCCAG106721.3791820023985772RNA PCR Primer, Index 1 (100% over 29bp)
CCTGTCTGAGCGTCGCTTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACC91551.1831344857532773RNA PCR Primer, Index 2 (100% over 34bp)
AATACCGGGTGCTGTAGGCTTTGGAATTCTCGGGTGCCAAGGAACTCCAGT62600.8090029361895703RNA PCR Primer, Index 1 (100% over 30bp)
GCCTGTCTGAGCGTCGCTTGGAATTCTCGGGTGCCAAGGAACTCCAGTCAC59240.7655804143749225RNA PCR Primer, Index 1 (100% over 33bp)
CGCCTGTCTGAGCGTCGCTTGGAATTCTCGGGTGCCAAGGAACTCCAGTCA59050.7631249741532609RNA PCR Primer, Index 1 (100% over 32bp)
TTCACAGTGGCTAAGTTCTGTGGAATTCTCGGGTGCCAAGGAACTCCAGTC56690.7326258219263058RNA PCR Primer, Index 1 (100% over 31bp)
CGCGACCTCAGATCAGACGTGGCGACCCGCTGATGGAATTCTCGGGTGCCA53140.6867478598899962No Hit
GGGAATACCGGGTGCTGTAGGCTTTTGGAATTCTCGGGTGCCAAGGAACTC45850.5925364955957156RNA PCR Primer, Index 1 (100% over 26bp)
GGGAATACCGGGTGCTGTAGGCTTTGGAATTCTCGGGTGCCAAGGAACTCC43520.5624250444563914RNA PCR Primer, Index 1 (100% over 27bp)
AATACCGGGTGCTGTAGGCTTTTGGAATTCTCGGGTGCCAAGGAACTCCAG42980.5554464248790372RNA PCR Primer, Index 1 (100% over 29bp)
ATACCGGGTGCTGTAGGCTTTGGAATTCTCGGGTGCCAAGGAACTCCAGTC41590.5374829411521442RNA PCR Primer, Index 1 (100% over 31bp)
GTCTGAGCGTCGCTTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCGTA40760.5267565443943591RNA PCR Primer, Index 22 (100% over 37bp)
TCGTGGTTGTAGTCCGTGCGAGAATACCATGGAATTCTCGGGTGCCAAGGA39540.5109900334973739RNA PCR Primer, Index 1 (100% over 22bp)
TGAGATGAAGCACTGTAGCTTGGAATTCTCGGGTGCCAAGGAACTCCAGTC38630.49922976717257356RNA PCR Primer, Index 1 (100% over 31bp)
GGAATACCGGGTGCTGTAGGCTTGGAATTCTCGGGTGCCAAGGAACTCCAG38260.4944481204251272RNA PCR Primer, Index 1 (100% over 29bp)
GAATACCGGGTGCTGTAGGCTTGGAATTCTCGGGTGCCAAGGAACTCCAGT36340.46963525081675694RNA PCR Primer, Index 1 (100% over 30bp)
GAATACCGGGTGCTGTAGGCTTATGGAATTCTCGGGTGCCAAGGAACTCCA32480.4197510442082627RNA PCR Primer, Index 1 (100% over 28bp)
GTACGACTCTTAGCGGTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCG32230.41652020181133953RNA PCR Primer, Index 2 (100% over 35bp)
ATACCGGGTGCTGTAGGCTTTTGGAATTCTCGGGTGCCAAGGAACTCCAGT32010.4136770605020471RNA PCR Primer, Index 1 (100% over 30bp)
GTTTCCGTAGTGTAGTGGTTATCACGTTCGCCTTGGAATTCTCGGGTGCCA31210.4033383648318928No Hit
CCGGGTGCTGTAGGCTTTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACC29640.3830486745792151RNA PCR Primer, Index 2 (100% over 34bp)
TACCGGGTGCTGTAGGCTTTGGAATTCTCGGGTGCCAAGGAACTCCAGTCA29320.3789131963111534RNA PCR Primer, Index 1 (100% over 32bp)
TCTGAGCGTCGCTTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCGTAC28880.37322691369256855RNA PCR Primer, Index 22 (100% over 38bp)
GCATTGGTGGTTCAGTGGTAGAATTCTCGCCTGCTGGAATTCTCGGGTGCC28540.36883296803275295No Hit
CTGGGAATACCGGGTGCTGTAGGCTTTTGGAATTCTCGGGTGCCAAGGAAC28480.36805756585749144RNA PCR Primer, Index 1 (100% over 24bp)
TCCTTCATTCCACCGGAGTCTGTGGAATTCTCGGGTGCCAAGGAACTCCAG28480.36805756585749144RNA PCR Primer, Index 1 (100% over 29bp)
TGTCTGAGCGTCGCTTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCGT26940.34815557669244446RNA PCR Primer, Index 12 (97% over 36bp)
ACCGGGTGCTGTAGGCTTTGGAATTCTCGGGTGCCAAGGAACTCCAGTCAC26640.34427856581613664RNA PCR Primer, Index 1 (100% over 33bp)
GGAATACCGGGTGCTGTAGGCTTATGGAATTCTCGGGTGCCAAGGAACTCC26210.3387215168934287RNA PCR Primer, Index 1 (100% over 27bp)
CCTGGGAATACCGGGTGCTGTAGGCTTTGGAATTCTCGGGTGCCAAGGAAC25040.3236011744758281RNA PCR Primer, Index 1 (100% over 24bp)
ATACCGGGTGCTGTAGGCTTATGGAATTCTCGGGTGCCAAGGAACTCCAGT24620.31817335924899715RNA PCR Primer, Index 1 (100% over 30bp)
AACATTCAACGCTGTCGGTGAGTGGAATTCTCGGGTGCCAAGGAACTCCAG24250.3133917125015508RNA PCR Primer, Index 1 (100% over 29bp)
GCATTGGTGGTTCAGTGGTAGAATTCTCGCCTTGGAATTCTCGGGTGCCAA24000.3101608701046276No Hit
AATACCGGGTGCTGTAGGCTTATGGAATTCTCGGGTGCCAAGGAACTCCAG23310.30124374508911955RNA PCR Primer, Index 1 (100% over 29bp)
CCTGGGAATACCGGGTGCTGTAGGCTTTTGGAATTCTCGGGTGCCAAGGAA23200.29982217443447334RNA PCR Primer, Index 1 (100% over 23bp)
TGGGAATACCGGGTGCTGTAGGCTTTTGGAATTCTCGGGTGCCAAGGAACT22620.2923266200736115RNA PCR Primer, Index 1 (100% over 25bp)
TACCGGGTGCTGTAGGCTTTTGGAATTCTCGGGTGCCAAGGAACTCCAGTC22570.2916804515942269RNA PCR Primer, Index 1 (100% over 31bp)
CCACGTTCCCGTGGTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCGTA21700.28043712005293414RNA PCR Primer, Index 22 (100% over 37bp)
TAGCTTATCAGACTGATGTTGATGGAATTCTCGGGTGCCAAGGAACTCCAG21620.2794032504859187RNA PCR Primer, Index 1 (100% over 29bp)
TGGGAATACCGGGTGCTGTAGGCTTTGGAATTCTCGGGTGCCAAGGAACTC20720.2677722178569952RNA PCR Primer, Index 1 (100% over 26bp)
CTGGGAATACCGGGTGCTGTAGGCTTTGGAATTCTCGGGTGCCAAGGAACT20510.2650583102435797RNA PCR Primer, Index 1 (100% over 25bp)
GGGGCCCAAGTCCTTCTGATCGAGGCCTGGAATTCTCGGGTGCCAAGGAAC20460.26441214176419503RNA PCR Primer, Index 1 (100% over 24bp)
GTTTCCGTAGTGTAGTGGTTATCACGTTCGCCTCTGGAATTCTCGGGTGCC20180.26079359827964105No Hit
AATACCGGGTGCTGTAGGCTTGGAATTCTCGGGTGCCAAGGAACTCCAGTC19740.25510731566105616RNA PCR Primer, Index 1 (100% over 31bp)
TGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCGTACGATCTCGTATGCC19360.25019643521773294RNA PCR Primer, Index 22 (100% over 51bp)
ACCGGGTGCTGTAGGCTTTTGGAATTCTCGGGTGCCAAGGAACTCCAGTCA19230.24851639717133286RNA PCR Primer, Index 1 (100% over 32bp)
GGGGCTACGCCTGTCTGAGCGTCGCTTGGAATTCTCGGGTGCCAAGGAACT19060.24631942434142506RNA PCR Primer, Index 1 (100% over 25bp)
TACCCTGTAGATCCGAATTTGTTGGAATTCTCGGGTGCCAAGGAACTCCAG18980.24528555477440964RNA PCR Primer, Index 1 (100% over 29bp)
ATGGGAGACCGCCTGGGAATACCGGGTGCTGTAGGCTTTGGAATTCTCGGG18740.24218394607336338No Hit
CCGGGTGCTGTAGGCTTTTGGAATTCTCGGGTGCCAAGGAACTCCAGTCAC18250.2358514949753939RNA PCR Primer, Index 1 (100% over 33bp)
TGGGGGGCCCAAGTCCTTCTGATCGAGGCCTGGAATTCTCGGGTGCCAAGG18240.23572226127951695Illumina Small RNA Adapter 2 (100% over 21bp)
TGAGGTAGTAGGTTGTATAGTTTGGAATTCTCGGGTGCCAAGGAACTCCAG17810.23016521235680906RNA PCR Primer, Index 1 (100% over 29bp)
CAACGGAATCCCAAAAGCAGCTGTGGAATTCTCGGGTGCCAAGGAACTCCA17640.22796823952690126RNA PCR Primer, Index 1 (100% over 28bp)
CCGGGGCTACGCCTGTCTGAGCGTCGCTTGGAATTCTCGGGTGCCAAGGAA17260.22305735908357802RNA PCR Primer, Index 1 (100% over 23bp)
TAGCTTATCAGACTGATGTTGACTGGAATTCTCGGGTGCCAAGGAACTCCA17040.22021421777428557RNA PCR Primer, Index 1 (100% over 28bp)
TGAGGTAGTAGTTTGTGCTGTTTGGAATTCTCGGGTGCCAAGGAACTCCAG16810.21724184276911626RNA PCR Primer, Index 1 (100% over 29bp)
CGGGTGCTGTAGGCTTTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCG16680.21556180472271616RNA PCR Primer, Index 2 (100% over 35bp)
AACATTCAACGCTGTCGGTGAGTTGGAATTCTCGGGTGCCAAGGAACTCCA16610.21465716885157768RNA PCR Primer, Index 1 (100% over 28bp)
TGAGGTAGTAGATTGTATAGTTTGGAATTCTCGGGTGCCAAGGAACTCCAG16600.21452793515570073RNA PCR Primer, Index 1 (100% over 29bp)
CGGGGCTACGCCTGTCTGAGCGTCGCTTGGAATTCTCGGGTGCCAAGGAAC16430.212330962325793RNA PCR Primer, Index 1 (100% over 24bp)
GTCTACGGCCATACCACCCTGAACGCGCCCGATGGAATTCTCGGGTGCCAA16010.206903147098962No Hit
ATACCGGGTGCTGTAGGCTTGGAATTCTCGGGTGCCAAGGAACTCCAGTCA15470.1999245275216079RNA PCR Primer, Index 1 (100% over 32bp)
GTCTACGGCCATACCACCCTGAACGCGCCCGTGGAATTCTCGGGTGCCAAG15060.19462594599065383No Hit
TCCCTGGTGGTCTAGTGGTTAGGATTCGGCGCTCTGGAATTCTCGGGTGCC14790.19113663620197674No Hit
CGCGACCTCAGATCAGACGTGGCGACCCGCTGAATGGAATTCTCGGGTGCC14460.1868719242380381No Hit
GGGGGCCCAAGTCCTTCTGATCGAGGCCTGGAATTCTCGGGTGCCAAGGAA14240.18402878292874572RNA PCR Primer, Index 1 (100% over 23bp)
AAGCTGCCAGTTGAAGAACTGTTGGAATTCTCGGGTGCCAAGGAACTCCAG13790.17821326661428394RNA PCR Primer, Index 1 (100% over 29bp)
TCCTTCTGATCGAGGCCTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACC12640.1633513915884372RNA PCR Primer, Index 2 (100% over 34bp)
CATTATTACTTTTGGTACGCGTGGAATTCTCGGGTGCCAAGGAACTCCAGT12630.16322215789256025RNA PCR Primer, Index 1 (100% over 30bp)
GAGACCGCCTGGGAATACCGGGTGCTGTAGGCTTTGGAATTCTCGGGTGCC12400.16024978288739092No Hit
CGCGACCTCAGATCAGACGTGGCGACCCGCTGAATTGGAATTCTCGGGTGC12400.16024978288739092No Hit
TTCGCGCTTTCCCCTGTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCG12360.15973284810388322RNA PCR Primer, Index 2 (100% over 35bp)
CGACACAAGGGTTTGTTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCG12190.15753587527397545RNA PCR Primer, Index 2 (100% over 35bp)
TTCAAGTAATCCAGGATAGGCTTGGAATTCTCGGGTGCCAAGGAACTCCAG12190.15753587527397545RNA PCR Primer, Index 1 (100% over 29bp)
TGAGATGAAGCACTGTAGCTCTGGAATTCTCGGGTGCCAAGGAACTCCAGT12030.1554681361399446RNA PCR Primer, Index 1 (100% over 30bp)
TACCGGGTGCTGTAGGCTTATGGAATTCTCGGGTGCCAAGGAACTCCAGTC11930.1541757991811753RNA PCR Primer, Index 1 (100% over 31bp)
TATTGCACTTGTCCCGGCCTGTTGGAATTCTCGGGTGCCAAGGAACTCCAG11490.14848951656259046RNA PCR Primer, Index 1 (100% over 29bp)
CTGTCTGAGCGTCGCTTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCG11290.1459048426450519RNA PCR Primer, Index 2 (100% over 35bp)
ATCTAAAGGTCCCTGGTTCGATCCCGGGTTTCGGCACCATGGAATTCTCGG11040.1426740002481287No Hit
CGGGTGCTGTAGGCTTTTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACC10980.14189859807286712RNA PCR Primer, Index 2 (100% over 34bp)
CGTTCGCGCTTTCCCCTGTGGAATTCTCGGGTGCCAAGGAACTCCAGTCAC10870.1404770274182209RNA PCR Primer, Index 1 (100% over 33bp)
GTTTGTGATGACTTACATGGAATTCTCGGGTGCCAAGGAACTCCAGTCACC10870.1404770274182209RNA PCR Primer, Index 2 (100% over 34bp)
CTCGACACAAGGGTTTGTTGGAATTCTCGGGTGCCAAGGAACTCCAGTCAC10730.13866775567594392RNA PCR Primer, Index 1 (100% over 33bp)
GTGGGGGGCCCAAGTCCTTCTGATCGAGGCCTGGAATTCTCGGGTGCCAAG10170.13143066870683592No Hit
CCGCCTGGGAATACCGGGTGCTGTAGGCTTTGGAATTCTCGGGTGCCAAGG10160.131301435010959Illumina Small RNA Adapter 2 (100% over 21bp)
ACCGGGTGCTGTAGGCTTATGGAATTCTCGGGTGCCAAGGAACTCCAGTCA10150.1311722013150821RNA PCR Primer, Index 1 (100% over 32bp)
ACACAAGGGTTTGTTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCGTA10150.1311722013150821RNA PCR Primer, Index 22 (100% over 37bp)
CTACGCCTGTCTGAGCGTCGCTTGGAATTCTCGGGTGCCAAGGAACTCCAG10110.1306552665315744RNA PCR Primer, Index 1 (100% over 29bp)
GCATTGGTGGTTCAGTGGTAGAATTCTCGCCTGGAATTCTCGGGTGCCAAG10030.12962139696455896No Hit
TACCCTGTAGAACCGAATTTGTGTGGAATTCTCGGGTGCCAAGGAACTCCA9880.12768289152640502RNA PCR Primer, Index 1 (100% over 28bp)
CGCGACCTCAGATCAGACGTGGCGACCCGCTGAATTTGGAATTCTCGGGTG9880.12768289152640502No Hit
CGTGGGGGGCCCAAGTCCTTCTGATCGAGGCCTGGAATTCTCGGGTGCCAA9680.12509821760886647No Hit
GTCTACGGCCATACCACCCTGAACGCGCCCTGGAATTCTCGGGTGCCAAGG9650.12471051652123569Illumina Small RNA Adapter 2 (100% over 21bp)
TCAGTGCACTACAGAACTTTGTTGGAATTCTCGGGTGCCAAGGAACTCCAG9480.1225135436913279RNA PCR Primer, Index 1 (100% over 29bp)
CCGGGTGCTGTAGGCTTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCG9400.12147967412431249RNA PCR Primer, Index 2 (100% over 35bp)
TCGACACAAGGGTTTGTTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACC9320.12044580455729706RNA PCR Primer, Index 2 (100% over 34bp)
TACCGGGTGCTGTAGGCTTGGAATTCTCGGGTGCCAAGGAACTCCAGTCAC9310.12031657086142011RNA PCR Primer, Index 1 (100% over 33bp)
TCCTTCATTCCACCGGAGTCTGTTGGAATTCTCGGGTGCCAAGGAACTCCA9300.1201873371655432RNA PCR Primer, Index 1 (100% over 28bp)
TACTTGGATGGGAGACCGCCTGGAATTCTCGGGTGCCAAGGAACTCCAGTC9160.11837806542326619RNA PCR Primer, Index 1 (100% over 31bp)
GTCTACGGCCATACCACCCTGAACGCGCCCGATCTGGAATTCTCGGGTGCC9080.11734419585625076No Hit
GCATTGGTGGTTCAGTGGTAGAATTCTCGCCTGTGGAATTCTCGGGTGCCA9050.11695649476861998No Hit
TTCGGGCGCCATGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCGTACGA8990.11618109259335843RNA PCR Primer, Index 22 (100% over 40bp)
TCCCTGTTCGGGCGCCATGGAATTCTCGGGTGCCAAGGAACTCCAGTCACC8840.11424258715520451RNA PCR Primer, Index 2 (100% over 34bp)
TTTCGGCACCATGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCGTACGA8810.11385488606757371RNA PCR Primer, Index 22 (100% over 40bp)
GTCTACGGCCATACCACCCTGAACGCGCCCGATTGGAATTCTCGGGTGCCA8530.11023634258301973No Hit
CGCGACCTCAGATCAGATGGAATTCTCGGGTGCCAAGGAACTCCAGTCACC8450.10920247301600429RNA PCR Primer, Index 2 (100% over 34bp)
GATGGGAGACCGCCTGGGAATACCGGGTGCTGTAGGCTTTGGAATTCTCGG8400.10855630453661966No Hit
ACCGGGTGCTGTAGGCTTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACC8380.10829783714486581RNA PCR Primer, Index 2 (100% over 34bp)
TCACGTCGGGGTCACCATGGAATTCTCGGGTGCCAAGGAACTCCAGTCACC8080.10442082626855795RNA PCR Primer, Index 2 (100% over 34bp)
CCTTCTGATCGAGGCCTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCG8040.10390389148505023RNA PCR Primer, Index 2 (100% over 35bp)
TCCCTGGTGGTCTAGTGGTTAGGATTCGGCGCTTGGAATTCTCGGGTGCCA7990.1032577230056656No Hit
CTGAGCGTCGCTTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCGTACG7920.10235308713452711RNA PCR Primer, Index 22 (100% over 39bp)
GTTAGTACTTGGATGGGAGACCGCCTGGAATTCTCGGGTGCCAAGGAACTC7880.1018361523510194RNA PCR Primer, Index 1 (100% over 26bp)
GGTGCTGTAGGCTTTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCGTA7840.10131921756751168RNA PCR Primer, Index 22 (100% over 37bp)

[FAIL]Adapter Content

Adapter graph