FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005732258

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005732258
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3992297
Sequences flagged as poor quality0
Sequence length51
%GC55

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TGGAATTCTCGGGTGCCAAGGAACTCCAGTCACTGACCAATCTCGTATGCC3559398.915644302014604RNA PCR Primer, Index 4 (100% over 51bp)
TCGTACGACTCTTAGCGGTGGAATTCTCGGGTGCCAAGGAACTCCAGTCAC1324683.318089811454408RNA PCR Primer, Index 1 (100% over 33bp)
TCCTTCATTCCACCGGAGTCTGTGGAATTCTCGGGTGCCAAGGAACTCCAG929312.32775767935101RNA PCR Primer, Index 1 (100% over 29bp)
GTACGACTCTTAGCGGTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACTG507621.271498588406624RNA PCR Primer, Index 2 (97% over 35bp)
CCACGTTCCCGTGGTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACTGAC500621.2539648227574252RNA PCR Primer, Index 4 (100% over 37bp)
GGAATACCGGGTGCTGTAGGCTTTTGGAATTCTCGGGTGCCAAGGAACTCC282290.7070866721589101RNA PCR Primer, Index 1 (100% over 27bp)
GTCTACGGCCATACCACCCTGAACGCGCCCGATGGAATTCTCGGGTGCCAA250960.6286105467604238No Hit
TAGCTTATCAGACTGATGTTGACTGGAATTCTCGGGTGCCAAGGAACTCCA221060.5537163192017027RNA PCR Primer, Index 1 (100% over 28bp)
GGAATACCGGGTGCTGTAGGCTTTGGAATTCTCGGGTGCCAAGGAACTCCA217990.5460265105526969RNA PCR Primer, Index 1 (100% over 28bp)
TGGGAATACCGGGTGCTGTAGGCTTTTGGAATTCTCGGGTGCCAAGGAACT213520.5348299487738513RNA PCR Primer, Index 1 (100% over 25bp)
ACCACGTTCCCGTGGTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACTGA182400.4568798363448411RNA PCR Primer, Index 2 (97% over 36bp)
GTAGTCGTGGCCTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACTGACCA172830.4329086738787219RNA PCR Primer, Index 4 (100% over 39bp)
TGGGAATACCGGGTGCTGTAGGCTTTGGAATTCTCGGGTGCCAAGGAACTC170730.4276485441839623RNA PCR Primer, Index 1 (100% over 26bp)
GGGAATACCGGGTGCTGTAGGCTTTTGGAATTCTCGGGTGCCAAGGAACTC164600.41229397512259236RNA PCR Primer, Index 1 (100% over 26bp)
TTGTACGACTCTTAGCGGTGGAATTCTCGGGTGCCAAGGAACTCCAGTCAC155030.3883228126564732RNA PCR Primer, Index 1 (100% over 33bp)
GCATTGGTGGTTCAGTGGTAGAATTCTCGCCTTGGAATTCTCGGGTGCCAA154870.3879220408702058No Hit
CCTGTCTGAGCGTCGCTTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACT154570.3871705937709544RNA PCR Primer, Index 3 (100% over 34bp)
GTCTACGGCCATACCACCCTGAACGCGCCCGATTGGAATTCTCGGGTGCCA149230.37379483540427977No Hit
GAATACCGGGTGCTGTAGGCTTTTGGAATTCTCGGGTGCCAAGGAACTCCA148330.3715404941065256RNA PCR Primer, Index 1 (100% over 28bp)
GTCTACGGCCATACCACCCTGAACGCGCCCGTGGAATTCTCGGGTGCCAAG146710.3674826797705682No Hit
TACCCTGTAGAACCGAATTTGTTGGAATTCTCGGGTGCCAAGGAACTCCAG143180.3586406522360436RNA PCR Primer, Index 1 (100% over 29bp)
ATACCGGGTGCTGTAGGCTTTGGAATTCTCGGGTGCCAAGGAACTCCAGTC139810.35019939648778636RNA PCR Primer, Index 1 (100% over 31bp)
GAATACCGGGTGCTGTAGGCTTTGGAATTCTCGGGTGCCAAGGAACTCCAG137730.3449893632663101RNA PCR Primer, Index 1 (100% over 29bp)
GTCTACGGCCATACCACCCTGAACGCGCCCGATCTGGAATTCTCGGGTGCC132300.33138817076986005No Hit
GGGAATACCGGGTGCTGTAGGCTTTGGAATTCTCGGGTGCCAAGGAACTCC131380.3290837329988225RNA PCR Primer, Index 1 (100% over 27bp)
TTCACAGTGGCTAAGTTCTGTGGAATTCTCGGGTGCCAAGGAACTCCAGTC123490.30932067428851107RNA PCR Primer, Index 1 (100% over 31bp)
GGAATTCTCGGGTGCCAAGGAACTCCAGTCACTGACCAATCTCGTATGCCG121790.30506247405941994RNA PCR Primer, Index 4 (100% over 51bp)
TGAGGTAGTAGGTTGTATAGTTTGGAATTCTCGGGTGCCAAGGAACTCCAG121550.30446131638001883RNA PCR Primer, Index 1 (100% over 29bp)
TCCTTCATTCCACCGGAGTCTGTTGGAATTCTCGGGTGCCAAGGAACTCCA121130.30340929044106685RNA PCR Primer, Index 1 (100% over 28bp)
ATACCGGGTGCTGTAGGCTTTTGGAATTCTCGGGTGCCAAGGAACTCCAGT120490.3018062032959973RNA PCR Primer, Index 1 (100% over 30bp)
CGACACAAGGGTTTGTTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACTG119630.29965205494480995RNA PCR Primer, Index 2 (97% over 35bp)
GTTTCCGTAGTGTAGTGGTTATCACGTTCGCCTTGGAATTCTCGGGTGCCA113650.28467320943306573No Hit
TAGCTTATCAGACTGATGTTGATGGAATTCTCGGGTGCCAAGGAACTCCAG110030.2756057477687657RNA PCR Primer, Index 1 (100% over 29bp)
CACCACGTTCCCGTGGTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACTG109210.2735517923641453RNA PCR Primer, Index 2 (97% over 35bp)
GTCTGAGCGTCGCTTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACTGAC104500.2617540729058985RNA PCR Primer, Index 4 (100% over 37bp)
TCGACACAAGGGTTTGTTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACT104070.26067699873030487RNA PCR Primer, Index 3 (100% over 34bp)
TCCAGGGTTCAAGTCCCTGTTCGGGCGCCATGGAATTCTCGGGTGCCAAGG104060.2606519504936632Illumina Small RNA Adapter 2 (100% over 21bp)
CGCGACCTCAGATCAGACGTGGCTGGAATTCTCGGGTGCCAAGGAACTCCA101730.25481571135614406RNA PCR Primer, Index 1 (100% over 28bp)
TACCGGGTGCTGTAGGCTTTGGAATTCTCGGGTGCCAAGGAACTCCAGTCA100710.2522607912186894RNA PCR Primer, Index 1 (100% over 32bp)
AATACCGGGTGCTGTAGGCTTTGGAATTCTCGGGTGCCAAGGAACTCCAGT100240.2510835240965289RNA PCR Primer, Index 1 (100% over 30bp)
CTCGACACAAGGGTTTGTTGGAATTCTCGGGTGCCAAGGAACTCCAGTCAC97530.24429545196662472RNA PCR Primer, Index 1 (100% over 33bp)
TCGTGGGTTCGAGCCCCACGTTGGGCGCCATGGAATTCTCGGGTGCCAAGG97490.24419525902005787Illumina Small RNA Adapter 2 (100% over 21bp)
GCATGGGTGGTTCAGTGGTAGAATTCTCGCCTTGGAATTCTCGGGTGCCAA97090.24319332955438935No Hit
TGTCTGAGCGTCGCTTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACTGA96240.24106422943984376RNA PCR Primer, Index 2 (97% over 36bp)
CACGTTGGGCGCCATGGAATTCTCGGGTGCCAAGGAACTCCAGTCACTGAC94680.23715670452373658RNA PCR Primer, Index 4 (100% over 37bp)
CAACGGAATCCCAAAAGCAGCTGTGGAATTCTCGGGTGCCAAGGAACTCCA92760.23234744308852773RNA PCR Primer, Index 1 (100% over 28bp)
GTCTACGGCCATACCACCCTGAACGCGCCTGGAATTCTCGGGTGCCAAGGA91120.22823953227928684RNA PCR Primer, Index 1 (100% over 22bp)
CAAGGGTTTGTTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACTGACCAA90640.22703721692048465RNA PCR Primer, Index 4 (100% over 40bp)
ACCGGGTGCTGTAGGCTTTGGAATTCTCGGGTGCCAAGGAACTCCAGTCAC89570.22435705559982136RNA PCR Primer, Index 1 (100% over 33bp)
TATTGCACTTGTCCCGGCCTGTTGGAATTCTCGGGTGCCAAGGAACTCCAG88740.2222780519585592RNA PCR Primer, Index 1 (100% over 29bp)
GTCTACGGCCATACCACCCTGAACGCGCCCTGGAATTCTCGGGTGCCAAGG87580.2193724565081205Illumina Small RNA Adapter 2 (100% over 21bp)
CCGGGTGCTGTAGGCTTTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACT86960.2178194658363343RNA PCR Primer, Index 3 (100% over 34bp)
TACCGGGTGCTGTAGGCTTTTGGAATTCTCGGGTGCCAAGGAACTCCAGTC85970.21533969040880477RNA PCR Primer, Index 1 (100% over 31bp)
CTGGGAATACCGGGTGCTGTAGGCTTTTGGAATTCTCGGGTGCCAAGGAAC85310.2136865067904517RNA PCR Primer, Index 1 (100% over 24bp)
CGCGACCTCAGATCAGACGTGGCGACCCGCTGATGGAATTCTCGGGTGCCA80380.2013377261260873No Hit
CTGTCTGAGCGTCGCTTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACTG78270.1960525481946859RNA PCR Primer, Index 2 (97% over 35bp)
CGCGACCTCAGATCAGACGTGGCGACCCGCTGAATTGGAATTCTCGGGTGC77180.19332229040073923No Hit
TGAGATGAAGCACTGTAGCTTGGAATTCTCGGGTGCCAAGGAACTCCAGTC75600.1893646690113486RNA PCR Primer, Index 1 (100% over 31bp)
AATACCGGGTGCTGTAGGCTTTTGGAATTCTCGGGTGCCAAGGAACTCCAG75270.18853807720217208RNA PCR Primer, Index 1 (100% over 29bp)
ACCGGGTGCTGTAGGCTTTTGGAATTCTCGGGTGCCAAGGAACTCCAGTCA75270.18853807720217208RNA PCR Primer, Index 1 (100% over 32bp)
GCATTGGTGGTTCAGTGGTAGAATTCTCGCCTGCTGGAATTCTCGGGTGCC72510.18162476388905938No Hit
TGAGAACTGAATTCCATAGGCTGTTGGAATTCTCGGGTGCCAAGGAACTCC72040.18044749676689883RNA PCR Primer, Index 1 (100% over 27bp)
CCGGGTGCTGTAGGCTTTTGGAATTCTCGGGTGCCAAGGAACTCCAGTCAC71670.1795207120111555RNA PCR Primer, Index 1 (100% over 33bp)
GTTTCCGTAGTGTAGTGGTTATCTGGAATTCTCGGGTGCCAAGGAACTCCA71480.17904479551496294RNA PCR Primer, Index 1 (100% over 28bp)
TCGTATGACTCTTAGCGGTGGAATTCTCGGGTGCCAAGGAACTCCAGTCAC70570.1767654059805671RNA PCR Primer, Index 1 (100% over 33bp)
CCACGTTGGGCGCCATGGAATTCTCGGGTGCCAAGGAACTCCAGTCACTGA69510.1741102928965455RNA PCR Primer, Index 2 (97% over 36bp)
GTCTACGGCCTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACTGACCAAT66770.1672470760567162RNA PCR Primer, Index 4 (100% over 41bp)
GGGGCTACGCCTGTCTGAGCGTCGCTTGGAATTCTCGGGTGCCAAGGAACT65860.16496768652232036RNA PCR Primer, Index 1 (100% over 25bp)
CGCGACCTCAGATCAGACGTGGCGACCCGCTGAATGGAATTCTCGGGTGCC65350.163690226453593No Hit
CGGGTGCTGTAGGCTTTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACTG64380.16126054749934687RNA PCR Primer, Index 2 (97% over 35bp)
TCCAGGGTTCAGGTCCCTGTTCGGGCGCCATGGAATTCTCGGGTGCCAAGG61890.1550235365755604Illumina Small RNA Adapter 2 (100% over 21bp)
TCGCTGGTTCGTTTCCGGCTCGAAGGACCATGGAATTCTCGGGTGCCAAGG59310.14856109152199848Illumina Small RNA Adapter 2 (100% over 21bp)
TGAGGTAGTAGGTTGTGTGGTTTGGAATTCTCGGGTGCCAAGGAACTCCAG58520.14658228082730318RNA PCR Primer, Index 1 (100% over 29bp)
CTGGGAATACCGGGTGCTGTAGGCTTTGGAATTCTCGGGTGCCAAGGAACT57550.14415260187305703RNA PCR Primer, Index 1 (100% over 25bp)
GTGAAATGTTTAGGACCACTAGTGGAATTCTCGGGTGCCAAGGAACTCCAG57530.1441025053997736RNA PCR Primer, Index 1 (100% over 29bp)
GCATTGGTGGTTCAGTGGTAGAATTCTCGCCTGGAATTCTCGGGTGCCAAG57390.14375183008678963No Hit
CACAAGGGTTTGTTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACTGACC56370.14119690994933493RNA PCR Primer, Index 4 (100% over 38bp)
CATGGAATTCTCGGGTGCCAAGGAACTCCAGTCACTGACCAATCTCGTATG53360.1336573907201794RNA PCR Primer, Index 4 (100% over 49bp)
CGGGTGCTGTAGGCTTTTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACT52920.13255526830794404RNA PCR Primer, Index 3 (100% over 34bp)
ACACAAGGGTTTGTTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACTGAC51240.12834716455213627RNA PCR Primer, Index 4 (100% over 37bp)
CGCCTGTCTGAGCGTCGCTTGGAATTCTCGGGTGCCAAGGAACTCCAGTCA51230.12832211631549456RNA PCR Primer, Index 1 (100% over 32bp)
TTCACAGTGGCTAAGTTCTGCTGGAATTCTCGGGTGCCAAGGAACTCCAGT51040.12784619981930204RNA PCR Primer, Index 1 (100% over 30bp)
TCTGAGCGTCGCTTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACTGACC50510.12651864327729126RNA PCR Primer, Index 4 (100% over 38bp)
CTGTTCGGGCGCCATGGAATTCTCGGGTGCCAAGGAACTCCAGTCACTGAC49930.1250658455520719RNA PCR Primer, Index 4 (100% over 37bp)
TAGCAGCACGTAAATATTGGCGTGGAATTCTCGGGTGCCAAGGAACTCCAG48920.1225359736512589RNA PCR Primer, Index 1 (100% over 29bp)
AACCCGTAGATCCGAACTTGTGTGGAATTCTCGGGTGCCAAGGAACTCCAG47450.11885388286492714RNA PCR Primer, Index 1 (100% over 29bp)
GTAGTCGTGGCCGAGTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACTGA47410.11875368991836029RNA PCR Primer, Index 2 (97% over 36bp)
GGGTGCTGTAGGCTTTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACTGA46650.11685002393359012RNA PCR Primer, Index 2 (97% over 36bp)
GCCTGTCTGAGCGTCGCTTGGAATTCTCGGGTGCCAAGGAACTCCAGTCAC46100.11547237091829592RNA PCR Primer, Index 1 (100% over 33bp)
GCATTGTGGTTCAGTGGTAGAATTCTCGCCTTGGAATTCTCGGGTGCCAAG45970.11514674384195363No Hit
TGAGGTAGTAGTTTGTGCTGTTTGGAATTCTCGGGTGCCAAGGAACTCCAG45080.1129174507808412RNA PCR Primer, Index 1 (100% over 29bp)
TTCAAGTAATCCAGGATAGGCTTGGAATTCTCGGGTGCCAAGGAACTCCAG44860.11236638957472353RNA PCR Primer, Index 1 (100% over 29bp)
CGCGACCTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACTGACCAATCTC44750.1120908589716647RNA PCR Primer, Index 4 (100% over 44bp)
TACCTGGTTGATCCTGCCTGGAATTCTCGGGTGCCAAGGAACTCCAGTCAC44230.11078835066629562RNA PCR Primer, Index 1 (100% over 33bp)
ATACCGGGTGCTGTAGGCTTGGAATTCTCGGGTGCCAAGGAACTCCAGTCA43450.10883458820824202RNA PCR Primer, Index 1 (100% over 32bp)
TGAAATGTTTAGGACCACTAGTGGAATTCTCGGGTGCCAAGGAACTCCAGT43380.10865925055175005RNA PCR Primer, Index 1 (100% over 30bp)
TCGCTGGTTCGATTCCGGCTCGAAGGACCATGGAATTCTCGGGTGCCAAGG43310.10848391289525804Illumina Small RNA Adapter 2 (100% over 21bp)
TCCTTCATTCCACCGGAGTCTTGGAATTCTCGGGTGCCAAGGAACTCCAGT43250.10833362347540776RNA PCR Primer, Index 1 (100% over 30bp)
CATTATTACTTTTGGTACGCGTGGAATTCTCGGGTGCCAAGGAACTCCAGT42100.1054530762616108RNA PCR Primer, Index 1 (100% over 30bp)
GTTTGTGATGACTTACATGGAATTCTCGGGTGCCAAGGAACTCCAGTCACT41990.10517754565855196RNA PCR Primer, Index 3 (100% over 34bp)
TGAGGTAGTAGATTGTATAGTTTGGAATTCTCGGGTGCCAAGGAACTCCAG41710.10447619503258398RNA PCR Primer, Index 1 (100% over 29bp)
CCCACGTTGGGCGCCATGGAATTCTCGGGTGCCAAGGAACTCCAGTCACTG40190.10066886306304365RNA PCR Primer, Index 2 (97% over 35bp)

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATCTACG351.1648808E-745.2319561
AGTAAGG2350.045.2319561
CTATACG206.859432E-445.2319531
TTATAAA206.859432E-445.2319531
TCGTAGG406.519258E-945.2319531
GTCTACG146650.045.185691
TCCTTCA128850.045.1792951
CCGTTTA351.2136115E-744.9968417
ACGATGT650.044.9957216
AAACCCA453.8562575E-1044.9957216
GCAGATA453.8562575E-1044.9957216
GTAACGA650.044.9957213
CACCTTA207.038687E-444.99571626
TCGTGTA207.038687E-444.99571612
AATAAGC1600.044.99571615
CGTAAAT6750.044.994039
CTATGGC800.044.9940263
CACATAA253.8955986E-544.9940267
GCTATAC2700.044.9940268
ACATTAA253.8955986E-544.9940269
Produced by FastQC (version 0.11.5)