FastQCFastQC Report
Wed 20 Apr 2022
EGAF00005732289

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005732289
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences63512879
Sequences flagged as poor quality0
Sequence length100
%GC49

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTATCG72900.017.404523
GTATCGA78400.016.9023024
CCCGTAT114950.013.8218731
TAAGGCG157700.012.3361718
CGGTAAT50250.011.0383741
CCGTATC148900.010.4785862
CCGGTAA55800.010.2774361
CTCGGAT138600.09.564121
AGATCGG496150.09.29171594
CAATCGG90800.09.2636044
GACGCTA67750.09.1554024
CCCGGTT146650.09.1032291
ATCGAAG181250.09.0481596
GGATTAA266050.08.9724964
CTTGGAT468850.08.8428821
GATCGGA449950.08.63736194
CCGGTAT56650.08.6296381
CCCGGAT195200.08.5247691
TATCGAA186800.08.4271515
ACGCTAC78550.08.2555445
Produced by FastQC (version 0.11.5)