FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005732291

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005732291
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences86634596
Sequences flagged as poor quality0
Sequence length51
%GC51

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCGTATG115150.027.47316643
CGTATGC120600.025.76491444
CTCGTAT122150.025.01609242
AATCTCG138250.022.9496439
TCTCGTA147350.021.08931541
ATCTCGT157350.019.90646640
ACACGTC185400.019.0878913
ACGTCTG187850.018.81494315
CGTATCG62750.018.2868753
CACGTCT196100.018.03491814
CCCGTAT69400.017.969511
GTATGCC181900.017.36646745
GTATCGA65300.017.228014
CACACGT210600.016.82523512
GACCAAT206950.016.77818535
ACCAATC201800.016.35875336
GATCGGA233850.015.0647821
GCACACG247900.014.66563211
ATCGGAA248850.014.1134852
TCGGAAG256850.013.963423
Produced by FastQC (version 0.11.5)