FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005732303

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005732303
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences67197995
Sequences flagged as poor quality0
Sequence length51
%GC50

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTATGC63500.021.53347444
TCGTATG67900.020.46778343
CTCGTAT72450.018.66663742
TATCTCG78100.017.28827739
ACCGATG85400.016.20935232
TCTCGTA86800.016.04622841
CACCGAT95150.014.57251131
CGATGTA94400.014.56681934
ATCTCGT97000.014.35889340
CCCGTAT42650.013.6143491
CGTATCG39900.013.4831423
ACACGTC117600.013.16950813
CCGATGT107800.012.71302533
TCACCGA123700.012.55978730
ACGTCTG121550.012.52079915
GTATCGA45200.012.3977454
GTATGCC115600.012.37148445
CACGTCT129700.011.854712514
GATCGGA122300.011.4752991
GTCACCG134600.011.39267229
Produced by FastQC (version 0.11.5)