FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005732305

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005732305
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences80554532
Sequences flagged as poor quality0
Sequence length51
%GC51

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACAGTTCCGTATCTCGTATG1166660.14482859884283109TruSeq Adapter, Index 14 (97% over 44bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCGTATG167150.036.52072545
CTCGTAT211250.028.73662844
CGTATCT225300.027.53417839
TATCTCG227100.027.23808141
TTCCGTA241500.026.31144136
TCTCGTA232100.026.24315843
GTTCCGT244250.026.08842835
GATCGGA251250.025.9453751
CCGTATC247600.025.16362238
AGTTCCG259450.024.96006834
ATCTCGT248150.024.88201142
ACACGTC263500.024.82903713
TCCGTAT257150.024.64804837
ATCGGAA266450.024.5156692
ACGTCTG291800.022.544615
CACGTCT292100.022.49833714
TCGGAAG307000.021.4449713
CGTCTGA310100.021.24370416
CACACGT310850.021.09029212
GTATCTC301800.020.80093640
Produced by FastQC (version 0.11.5)