FastQCFastQC Report
Wed 20 Apr 2022
EGAF00005732307

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005732307
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences108907187
Sequences flagged as poor quality0
Sequence length51
%GC48

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCGTATG153350.030.27022645
CTCGTAT176550.025.14759644
TCTCGTA196900.022.68646643
AATCTCG209900.021.80671941
ATCTCGT209550.021.37050842
GATCGGA233450.021.1523081
ACACGTC242350.020.6178113
ACGTCTG266150.019.03643815
TCGGAAG267450.018.6358533
CACGTCT273950.018.3876914
ATCGGAA275650.017.8942412
CGACCGT52650.017.4064031
CACACGT296200.016.9527812
GTATCGA95450.016.360524
AGTCAAC313400.016.05364434
GCACACG326600.015.70540211
CGTATCG103400.015.3856923
CGGAAGA353800.014.4753144
CCCGTAT116450.013.9028041
GTCAACA369100.013.7161235
Produced by FastQC (version 0.11.5)