FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005732310

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005732310
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences27956659
Sequences flagged as poor quality0
Sequence length51
%GC49

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTATCG10600.012.3067823
GTCGCCG35400.011.05296845
TGGTCGC32850.010.81593343
CGCAGAT32550.09.5620821
CGCGTAA2657.419112E-59.4303911
CGCCAAT21750.09.4008281
CGGGAAT35250.09.2163681
CGACAAT14550.09.0562391
GTAGATC44000.08.995007532
GTATCGA13700.08.8647244
CGGCAAT17350.08.7732041
GCGTCGT42700.08.74400512
GGTCGCC40400.08.73886744
CTCGAAT29150.08.7289571
AGCGTCG44500.08.59315211
CTCGGAT34800.08.4215761
CCCGTAT17900.08.3767161
CGGAAAT37500.08.2998871
TCGGGTC22550.08.2613342
CGTCGTG45650.08.22821913
Produced by FastQC (version 0.11.5)