FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005732312

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005732312
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences31952684
Sequences flagged as poor quality0
Sequence length51
%GC49

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GATCGGA41400.012.9108471
AGCGTCG38550.011.90278510
GCGTCGT34650.011.29506711
TCGCCGT29650.011.14799445
CGCGTAA6700.010.8633161
CGTCGTG38700.010.46075112
CGCAAAT20950.010.2054231
AGAGCGT46700.09.7771698
GTAGATC47800.09.73782931
ATCGGAA55500.09.7127582
CGCGAAT6150.09.61581
TCGTGTA40600.09.52752214
TGGTCGC39200.09.41060642
GGTCGCC46950.09.33929343
CGTGTAG48150.09.20126215
GTCGCCG47900.09.2008844
GTCGTGT43450.09.00659813
TCGGAAG65900.08.600023
CGGGAAT35850.08.5650871
CGACAAT18600.08.5599711
Produced by FastQC (version 0.11.5)