FastQCFastQC Report
Wed 20 Apr 2022
EGAF00005732324

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005732324
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences67924078
Sequences flagged as poor quality0
Sequence length100
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTATCG51300.010.4425273
CGCCAAT105900.09.1057751
CGGAAAT199000.09.0059791
GTATCGA64950.08.9714894
CGCAAAT86300.08.8300511
TAGATCG67800.08.82148594
CGCGTTT40350.08.7433271
GATCGGA318450.08.63652994
CCGGGAT238300.08.547181
CTCGGAT179000.08.5406191
AGATCGG377700.08.52858194
CGGGGAT190600.08.4403841
GTTCGAT102350.08.318591
CGGGGTT233650.08.1938431
CCGGCAT168250.08.0798221
CGTCGAT50200.07.96478751
CGCGAAT27200.07.9551421
CTCGAAT128400.07.839851
CCCGGAT212200.07.758571
GTCCGAT52150.07.75716731
Produced by FastQC (version 0.11.5)