FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005732327

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005732327
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences80509161
Sequences flagged as poor quality0
Sequence length51
%GC52

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCGTATG80100.027.2761145
CTCGTAT100400.021.08851244
TCTCGTA117900.017.7678943
ACCCGTC139200.017.0229832
ATCTCGT131750.016.00299842
CGTCCCG149500.015.98353535
ACACGTC158200.015.47286113
GATCGGA156100.015.371661
CCCGATC145100.015.01084838
TCCCGAT151050.014.79133937
GTCCCGA161550.014.56860636
ATCGGAA167500.014.3069362
ACGTCTG169500.014.26883715
CACCCGT174250.013.68947431
CCGATCT157000.013.54326939
CACGTCT183000.013.38833614
CGTATGC50850.012.5274291
CGTATCG41400.012.5001763
TCACCCG192100.012.47603230
TCGGAAG200300.012.4352743
Produced by FastQC (version 0.11.5)