FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005732328

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005732328
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences80509161
Sequences flagged as poor quality0
Sequence length51
%GC52

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTATCG44150.012.9629833
GTATCGA45150.012.9259954
CCCGTAT55150.012.134461
CCGTATC76400.09.1415752
TCAATCG46150.08.8370813
AGCGTCG79300.08.73673711
GTAGATC86100.08.38479832
CAATCGG49650.08.3506564
CGTCGTG88000.08.20948813
TGGTCGC84100.08.10304343
CCGGGAT122150.08.0427131
CGGGGAT113200.08.0018261
CGCGGTT51900.07.94498831
CGCGATT13900.07.94311431
TATCGAA80900.07.8806925
CGGCGAT76750.07.80931231
CCCGGTT122050.07.75391531
CGACAAT29700.07.73843671
CGCGGAT47000.07.62269971
TAAGGCG76200.07.5881788
Produced by FastQC (version 0.11.5)