FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005732339

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005732339
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences63963285
Sequences flagged as poor quality0
Sequence length51
%GC50

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCGTATG61300.022.57176445
CTCGTAT73650.017.3207244
TCTCGTA86650.014.54032443
CGTATCG32650.014.1354923
CGTCCCG109550.013.55601435
ACCCGTC110300.013.52386432
GTCCCGA114650.012.93492136
GTATCGA39100.012.7743444
CCCGATC99450.012.6944738
ACACGTC122700.012.52151113
ATCTCGT100500.012.42559442
TCCCGAT109800.012.35854637
GATCGGA116450.012.2845041
CCGATCT106550.011.65846639
CCCGTAT45450.011.4003281
ACGTCTG138500.011.20730815
CACCCGT134000.011.18250231
ATCGGAA130700.010.9553412
CACGTCT145500.010.72989414
CCGTATC49000.010.2919172
Produced by FastQC (version 0.11.5)