FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005732348

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005732348
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences76079881
Sequences flagged as poor quality0
Sequence length51
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCGTCGT68750.014.0382912
GATCGGA71650.012.9857612
GTATCGA51900.012.6771054
CGTCGTG89900.011.61699713
CGTATCG58500.011.4006193
AGCGTCG84050.011.23959111
TGGTCGC82450.011.15372143
CCCGTAT63750.011.0980271
AGATCGG83550.010.7332921
GTAGATC93450.010.70659232
ATCGGAA99850.09.22927953
GTCGCCG104150.09.17520145
TCTCGGT110000.09.13704337
AGAGCGT107250.08.9761429
CCGTATC82900.08.9135942
CCGGGAT119750.08.56115151
ATCGAAG123750.08.4598796
AATCGGG87400.08.3789465
CGGGGAT115350.08.067311
GTGGTCG120000.08.03824442
Produced by FastQC (version 0.11.5)