FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005732361

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005732361
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences73642908
Sequences flagged as poor quality0
Sequence length51
%GC51

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCGTATG56800.020.67626245
CTCGTAT69900.016.70544
CGTATCT80500.014.92814439
CGTATCG44000.014.8378613
TTCCGTA84200.014.646173536
TCTCGTA83250.014.24239443
GTATCGA49750.014.0193884
GTTCCGT89050.013.87215735
CCCGTAT54300.013.7737351
TATCTCG92250.013.68512241
ATCTCGT98050.012.57567942
AGTTCCG108100.011.75962634
CGACTAA54300.011.72688728
CCGTATC106250.011.52206738
ACACGTC122750.010.68383813
TCCGTAT122000.010.12670637
GATCGGA119650.09.8469671
ACGTCTG135650.09.61853215
CACGTCT143800.09.1046314
AACCGAC74450.08.76407625
Produced by FastQC (version 0.11.5)