FastQCFastQC Report
Mon 30 May 2022
EGAF00005732394

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005732394
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences295832035
Sequences flagged as poor quality0
Sequence length8-151
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AAGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT144250154.876082808273282Clontech SMART CDS Primer II A (100% over 26bp)
GCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT16626230.5620158749879809Clontech SMART CDS Primer II A (100% over 24bp)
AGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT9540560.32249921817966737Clontech SMART CDS Primer II A (100% over 25bp)
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG7481890.25291006770108587No Hit
CAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTT3056280.10331132664520258Clontech SMART CDS Primer II A (100% over 23bp)

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)