FastQCFastQC Report
Mon 30 May 2022
EGAF00005732420

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005732420
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences333613965
Sequences flagged as poor quality0
Sequence length8-151
%GC45

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[FAIL]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AAGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT3850901211.54298561812303Clontech SMART CDS Primer II A (100% over 26bp)
GCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT28932990.8672595585139848Clontech SMART CDS Primer II A (100% over 24bp)
AGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT21589280.6471335814734254Clontech SMART CDS Primer II A (100% over 25bp)
AAGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTA4599280.13786233438998874Clontech SMART CDS Primer II A (100% over 26bp)
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG4054120.12152129183201309No Hit
AAGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTG3980420.11931215169604786Clontech SMART CDS Primer II A (100% over 26bp)

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)