FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005732444

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005732444
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences81081195
Sequences flagged as poor quality0
Sequence length101
%GC49

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CATAATGGCGAGGCGTCCATCTCGAAGGAGTCGCCAGCGATAACCGGAGT2937640.3623084242899972No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATGGCGA627600.058.5809675
TAATGGC666550.055.3746223
TGGCGAG577500.054.132516
AATGGCG696750.052.8176884
CATAATG719200.051.760841
ATAATGG718700.051.637172
GCGAGGC611800.047.364078
GCGATAA362800.040.54370536-37
GGCGAGG730200.039.9247447
CGATAAC366350.039.83238-39
CGAGGCG742550.039.0368849
ATAACCG375550.038.95773740-41
AGCGATA379950.038.43240436-37
TAACCGG382500.038.41129740-41
TAAGACG402550.036.5624564-65
CGGAGTA408300.035.78774344-45
CGACCAA421000.034.5102470-71
GAGTCGC435400.033.4269928-29
AGTCGCC445300.032.95046628-29
AACCGGA448700.032.6126842-43
Produced by FastQC (version 0.11.5)