FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005732448

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005732448
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences77658242
Sequences flagged as poor quality0
Sequence length101
%GC49

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCGCCGT254000.044.1055075
CGCCGTG264300.032.6562736
CGTGGCG230100.029.3429079
CCCGCCG398700.028.8008844
CGCCGTT94550.028.0531336
CGATAAC121400.027.95161838-39
GCGATAA120400.027.90556536-37
AGGCCCG423500.027.0849361
ATAACCG126950.026.76612740-41
GGCCCGC439850.026.346492
TAACCGG137250.024.8611840-41
AGCGATA137900.024.63955336-37
TAAGACG146600.022.98044264-65
CGGAGTA152200.022.0409544-45
CGACCAA165050.020.84121370-71
TTCGCGT64650.020.62455418-19
CCGTTGA131050.020.4208348
TACGCGC69400.019.38711538-39
TCGCGTT68550.019.38216820-21
CGCGTTT69900.019.2114920-21
Produced by FastQC (version 0.11.5)