FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005732450

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005732450
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences103201803
Sequences flagged as poor quality0
Sequence length101
%GC50

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GACAAAGAGAAGGCGTCCATCTCGAAGGAGTCGCCAGCGATAACCGGAGT1295980.1255772634127332No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCGATAA211900.032.44412636-37
CGATAAC213100.031.02447738-39
ATAACCG217100.030.43105740-41
TAACCGG226650.030.33280640-41
AGCGATA236650.028.10768336-37
TAAGACG246750.027.80357664-65
CGGAGTA264150.025.83789644-45
CGACCAA267950.024.80612870-71
GAGTCGC303800.022.04341328-29
AGTCGCC318200.021.7548428-29
AACCGGA306850.021.73145542-43
GAAGGCG622250.021.6691049
AGACGAC322450.021.6371166-67
ATCTCGA340100.020.52927818-19
AAGGCGT340450.019.65634510-11
GTCGCCA359700.018.61104830-31
GATAACC380450.018.48869138-39
ACCGGAG378700.018.44867742-43
CGTCCAT373400.018.1449714-15
CGAAGGA389200.018.12794122-23
Produced by FastQC (version 0.11.5)