FastQCFastQC Report
Wed 20 Apr 2022
EGAF00005732454

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005732454
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences106037163
Sequences flagged as poor quality0
Sequence length101
%GC52

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ACGTTCTGTCGGGCGTCCATCTCGAAGGAGTCGCCAGCGATAACCGGAGT1760370.1660144377872501No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCGGGCG393850.043.929999
ACGTTCT577100.040.1670231
CGTTCTG585900.039.7119182
TGTCGGG483450.036.063017
GCGATAA250650.035.756136-37
CTGTCGG530850.035.534376
CGATAAC252300.035.3059938-39
GTCGGGC513200.034.065098
ATAACCG261400.034.0046240-41
AGCGATA276900.032.1778636-37
TAACCGG281200.031.94789540-41
TCTGTCG771200.030.4178355
TAAGACG304500.029.49742364-65
CGGAGTA326150.027.41435844-45
CGACCAA327700.027.00267670-71
AACCGGA366250.024.5230842-43
GTTCTGT970300.024.3548743
AGACGAC379250.023.84649566-67
GTCGTGA182900.023.824488
GAGTCGC380300.023.59821328-29
Produced by FastQC (version 0.11.5)