FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005732457

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005732457
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences90521525
Sequences flagged as poor quality0
Sequence length101
%GC52

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TCTTAATTGAGGGCGTCCATCTCGAAGGAGTCGCCAGCGATAACCGGAGT3017760.33337485200343236No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGATCTA931100.085.1009758
CCGATCT3648100.082.607937
TTCCGAT3696600.082.564375
TCCGATC3700850.082.444636
CTTCCGA3890400.078.808914
TCTTCCG3934000.077.715833
GCTCTTC4331750.070.960181
CGATCTG1067650.067.2061168
CGATCTT1216500.065.440178
CTCTTCC5274800.058.4170532
GATCTAA433800.057.773449
GATCTAG451900.053.7463659
TAATTGA731150.050.465394
TCTTAAT767000.048.7285161
AATTGAG762050.048.406745
CTTAATT781800.047.7169722
GATCTAT339700.044.8507279
CGATCTC1659650.043.2478838
GATCTTA403100.039.104369
ATTGAGG824400.038.907576
Produced by FastQC (version 0.11.5)