FastQCFastQC Report
Wed 20 Apr 2022
EGAF00005732468

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005732468
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences103682647
Sequences flagged as poor quality0
Sequence length101
%GC50

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ATATACGCAGTGGCGTCCATCTCGAAGGAGTCGCCAGCGATAACCGGAGT2868320.2766441717098523No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATACGC438700.085.334712
ATATACG451550.082.8905261
ATACGCA490650.076.366533
TACGCAG529100.070.908224
ACGCAGT587700.063.8443265
GCGATAA381000.038.2699836-37
CGATAAC388050.037.49533538-39
ATAACCG391800.037.17963840-41
CGCAGTG857300.036.8527836
AGCGATA406450.035.6925736-37
TAACCGG416600.035.23427240-41
TAAGACG439900.033.24788364-65
AGTGGCG880250.033.1515169
CGGAGTA439150.032.9506644-45
CGACCAA460100.031.57766770-71
GAGTCGC473550.030.61590628-29
AGTCGCC483900.030.17700628-29
AACCGGA491050.029.63731642-43
AGACGAC505200.029.03064566-67
ATCTCGA512550.028.87866418-19
Produced by FastQC (version 0.11.5)