FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005732472

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005732472
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences100964897
Sequences flagged as poor quality0
Sequence length101
%GC51

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CAGCGCTGGTTGGCGTCCATCTCGAAGGAGTCGCCAGCGATAACCGGAGT7661490.7588271000761779No Hit
CAGCGCTGGTTTGAATTGTTCGCGTTTACCTTGCGTGTACGCGCAGGAAA2824700.279770502811487No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTTGGCG1021350.074.861189
CGCTGGT1583250.071.115274
CAGCGCT1659950.067.9945451
AGCGCTG1663600.067.8394242
GCGCTGG1851450.061.0776633
GCTGGTT1969450.057.397435
GGTTGGC1342050.057.13858
TGGTTGG1476350.052.123287
CTGGTTG1687900.050.4313476
GCGATAA878550.043.5292536-37
CGATAAC880200.043.40287838-39
ATAACCG892950.042.96392440-41
AGCGATA898150.042.46298236-37
TAACCGG943800.040.7497740-41
CGGAGTA932450.040.70316744-45
TAAGACG950550.040.44734664-65
GAGTCGC977400.039.29428528-29
AGTCGCC985150.039.03578628-29
GGTTTGA732650.038.763098
CGACCAA994900.038.5228670-71
Produced by FastQC (version 0.11.5)