FastQCFastQC Report
Wed 20 Apr 2022
EGAF00005732483

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005732483
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences96726689
Sequences flagged as poor quality0
Sequence length101
%GC50

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ATTTGTGGTATGGCGTCCATCTCGAAGGAGTCGCCAGCGATAACCGGAGT7798700.8062614445533227No Hit
ATTTGTGGTATTGAATTGTTCGCGTTTACCTTGCGTGTACGCGCAGGAAA1822720.18844023493867346No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATGGCG968050.083.230659
GTGGTAT1455800.071.6521155
GTATGGC1128050.071.57838
GGTATGG1224950.066.0336767
TGTGGTA1623200.064.278694
ATTTGTG1679050.062.2728271
TTGTGGT1753100.059.4588783
TGGTATG1538500.055.721796
TTTGTGG1920200.054.507752
GCGATAA949250.043.11465536-37
CGATAAC952050.042.44923438-39
ATAACCG955150.042.28991340-41
TAACCGG979900.041.68469240-41
AGCGATA972500.041.57588236-37
GTATTGA467150.041.121288
TAAGACG1000450.040.70992764-65
GGTATTG476650.040.6465157
CGGAGTA994450.040.3344944-45
CGACCAA1033200.039.02447570-71
AGTCGCC1035950.038.99305328-29
Produced by FastQC (version 0.11.5)