FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005732489

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005732489
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences70606882
Sequences flagged as poor quality0
Sequence length101
%GC50

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CATAATGGCGAGGCGTCCATCTCGAAGGAGTCGCCAGCGATAACCGGAGT2134530.30231189078707654No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATGGCGA555100.057.6774335
TAATGGC585150.054.931333
CATAATG605050.053.4970861
AATGGCG611100.052.4573334
ATAATGG617600.052.292242
TGGCGAG500200.050.9092526
GCGAGGC555050.042.2494778
CGAGGCG592750.039.3765539
GCGATAA318750.038.0856736-37
CGATAAC319850.037.3829438-39
ATAACCG326150.036.61070640-41
AGCGATA329600.036.36398336-37
GGCGAGG663000.035.5054937
TAACCGG346450.034.9792240-41
TAAGACG352400.034.43425864-65
CGGAGTA354250.033.22907344-45
CGACCAA368900.032.21224270-71
CGATGAA215100.031.2730759
GAGTCGC385650.030.4228328-29
AGTCGCC390500.030.40953328-29
Produced by FastQC (version 0.11.5)