FastQCFastQC Report
Wed 20 Apr 2022
EGAF00005732490

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005732490
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences103526353
Sequences flagged as poor quality0
Sequence length101
%GC51

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AGCTGGCCTTCGGCGTCCATCTCGAAGGAGTCGCCAGCGATAACCGGAGT2525130.2439118086193957No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TTCGGCG376750.068.6306849
GCCTTCG649700.045.37566
CCTTCGG578400.045.0896267
GCGATAA351550.038.03933736-37
CGATAAC351650.037.73288338-39
ATAACCG359600.037.0526240-41
AGCGATA371400.035.78886836-37
TAACCGG381050.035.2099440-41
TTCGTAC101250.033.4462939
TAAGACG405300.033.00597864-65
CTTCGGC822350.032.0834358
CGGAGTA413350.031.90123244-45
TCGGCGT413950.031.44951810-11
CGACCAA425900.031.26082270-71
GAGTCGC460450.028.69517128-29
AACCGGA466800.028.61614842-43
AGACGAC471650.028.408866-67
AGTCGCC472600.028.16350228-29
TTCGCGT151550.027.941918-19
ATCTCGA486000.027.85962918-19
Produced by FastQC (version 0.11.5)