FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005732492

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005732492
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences91049002
Sequences flagged as poor quality0
Sequence length101
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CCGTAAATTACGGCGTCCATCTCGAAGGAGTCGCCAGCGATAACCGGAGT1860380.20432733573510228No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TACGGCG251450.074.390379
CGTAAAT321850.072.715922
CCGTAAA322400.072.468361
TTACGGC269650.069.4750758
ATTACGG275050.068.0765467
AATTACG337250.059.505896
GCGATAA260050.036.5977436-37
CGATAAC261650.036.27408638-39
ATAACCG266050.035.6763540-41
GTAAATT675850.035.0430763
TAACCGG275400.034.70655440-41
AGCGATA277000.034.28112436-37
TAAGACG297350.032.1414664-65
CGGAGTA304000.031.18792544-45
ACGGCGT304750.030.90405810-11
CGACCAA311200.030.48262870-71
GAGTCGC334000.028.50045428-29
AACCGGA340200.028.05079342-43
AGTCGCC343100.027.96599828-29
AAATTAC880700.027.4887035
Produced by FastQC (version 0.11.5)