FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005732497

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005732497
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences78153583
Sequences flagged as poor quality0
Sequence length101
%GC51

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TGACAACGCTGGGCGTCCATCTCGAAGGAGTCGCCAGCGATAACCGGAGT1340900.17157242809968162No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CAACGCT297750.059.1702044
ACAACGC311350.056.526763
GACAACG338400.052.1644062
AACGCTG360350.049.077825
GCGATAA218350.034.48097636-37
CGATAAC219550.032.6039138-39
ATAACCG221350.032.2693740-41
TAACCGG234150.031.98598340-41
TGACAAC572100.031.0935421
AGCGATA234400.030.57983236-37
TAAGACG245650.030.08973764-65
ACGCTGG518150.029.6037776
CGGAGTA261250.028.1131744-45
CGACCAA264600.026.99593570-71
AGTCGCC302450.024.746228-29
AGACGAC308150.024.3181766-67
GAGTCGC297050.024.04496828-29
AACCGGA302150.023.88247342-43
ATCTCGA320800.023.60393318-19
GATAACC334000.022.8040738-39
Produced by FastQC (version 0.11.5)