FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005732516

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005732516
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences86816839
Sequences flagged as poor quality0
Sequence length101
%GC54

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CATAATGGCGAGGCGTCCATCTCGAAGGAGTCGCCAGCGATAACCGGAGT2366310.27256348275937575No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CATAATG561600.054.445731
ATAATGG564200.054.2311172
TAATGGC565800.054.062933
ATGGCGA637650.047.872065
TGGCGAG621850.041.7484326
AATGGCG753850.040.4381644
CGATAAC311800.038.8108738-39
GCGATAA314700.038.7141336-37
ATAACCG317900.038.1142640-41
AGCGATA331200.036.5631536-37
TAACCGG335950.036.31391540-41
TAAGACG356100.034.04997364-65
GCGAGGC728800.033.5560388
CGGAGTA370800.032.34367844-45
CGACCAA388750.031.1095370-71
AACCGGA412250.029.48920642-43
GATAACC423450.028.98716238-39
GGTAATA423500.028.73236560-61
AGACGAC429750.028.45217966-67
ATCTCGA432700.028.39174718-19
Produced by FastQC (version 0.11.5)