FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005732518

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005732518
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences85042358
Sequences flagged as poor quality0
Sequence length101
%GC52

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CCTGGGAAACTGGCGTCCATCTCGAAGGAGTCGCCAGCGATAACCGGAGT2186060.25705543112997875No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCGATAA283800.038.18990336-37
CGATAAC285000.037.76896738-39
ATAACCG290450.037.1425540-41
ACTGGCG602250.036.1709149
AGCGATA301750.035.847336-37
TAACCGG312050.034.67809340-41
GGGAAAC874500.034.6067124
GGAAACT892550.034.010365
TAAGACG322550.033.73363564-65
CGGAGTA337700.031.79842644-45
CGACCAA342800.031.45574670-71
GAGTCGC386050.028.08645628-29
AGTCGCC389850.027.9527928-29
AACCGGA387850.027.81533642-43
AGACGAC391700.027.8143266-67
ATCTCGA401050.027.22552918-19
GATAACC403450.027.06873738-39
TTCGCGT118400.026.99598918-19
TCGCGTT123450.025.98790720-21
CGTCCAT430450.025.42533114-15
Produced by FastQC (version 0.11.5)