FastQCFastQC Report
Wed 20 Apr 2022
EGAF00005732524

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005732524
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences107330359
Sequences flagged as poor quality0
Sequence length101
%GC54

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TCACCTGTCGGGGCGTCCATCTCGAAGGAGTCGCCAGCGATAACCGGAGT4574970.42625125291903665No Hit
TCACCTGTCGGTGAATTGTTCGCGTTTACCTTGCGTGTACGCGCAGGAAA1387890.12931010507474403No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCTGTCG1019900.063.1796264
CTGTCGG1055950.061.1621065
TGTCGGG888850.057.411356
TCGGGGC1030050.045.1243638
GTCGGGG1079850.043.1708837
ACCTGTC1552750.041.8046153
CGGGGCG1117050.041.7034849
GCGATAA583600.040.65760436-37
CGATAAC584100.039.82678638-39
ATAACCG585600.039.74793640-41
AGCGATA607900.038.26273736-37
TGTCGGT369350.037.867536
TAACCGG632600.037.61696640-41
TAAGACG649550.036.25152664-65
CGGAGTA657600.035.77435744-45
ATCTCGA710850.033.5034618-19
GATAACC720050.033.24726538-39
CGACCAA697700.033.17297470-71
AGTCGCC728200.032.5192728-29
GAGTCGC724900.032.03499628-29
Produced by FastQC (version 0.11.5)