FastQCFastQC Report
Wed 20 Apr 2022
EGAF00005732526

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005732526
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences98532858
Sequences flagged as poor quality0
Sequence length101
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AGTGATTAGCAGGCGTCCATCTCGAAGGAGTCGCCAGCGATAACCGGAGT1798190.1824964825439246No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GATTAGC420800.057.5757524
TGATTAG483650.050.2121543
GTGATTA567750.043.1065942
ATTAGCA614100.039.1046185
GCGATAA282100.034.41507736-37
CGATAAC280900.033.79232838-39
ATAACCG289700.032.87232240-41
TAACCGG304950.031.96047240-41
AGCGATA301850.031.4552136-37
TTAGCAG642000.031.3165056
TAAGACG327000.029.6390464-65
CGGAGTA332750.028.77703944-45
TAGCAGG670950.027.8415787
CGACCAA341350.027.78045570-71
AACCGGA373800.025.57178542-43
GAGTCGC372350.025.56919728-29
AGTCGCC383300.025.40875228-29
AGACGAC385700.025.36222366-67
ATCTCGA401050.024.46762718-19
AGTGATT1071250.023.178691
Produced by FastQC (version 0.11.5)