FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005732530

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005732530
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences101597723
Sequences flagged as poor quality0
Sequence length101
%GC58

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TAAAGTCATGCGGCGTCCATCTCGAAGGAGTCGCCAGCGATAACCGGAGT5182940.5101433227986812No Hit
TAAAGTCATGCTGAATTGTTCGCGTTTACCTTGCGTGTACGCGCAGGAAA1875180.18456909708498093No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAAAGTC979550.077.4132841
AAGTCAT1177550.064.328473
AGTCATG1184950.063.8465734
TCATGCG935750.060.7534566
AAAGTCA1283900.059.214882
GTCATGC1308850.057.7646835
ATGCGGC1081200.049.982618
CATGCGG1138050.047.289657
TGCGGCG1212600.044.699689
GCGATAA653400.042.46441736-37
CGATAAC655300.041.38104638-39
ATGCGTA78950.041.025438
ATAACCG662500.040.9422440-41
AGCGATA671950.040.3096736-37
TAACCGG702850.039.5479640-41
TAAGACG701300.039.0008964-65
GATAACC745200.037.4341238-39
CGGAGTA746800.036.60633544-45
GGTAATA730600.036.50511660-61
GTAATAA759200.036.01841460-61
Produced by FastQC (version 0.11.5)