FastQCFastQC Report
Wed 20 Apr 2022
EGAF00005732531

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005732531
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences101597723
Sequences flagged as poor quality0
Sequence length101
%GC58

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TAAAGTCATGCGGCGTCCATCTCGAAGGAGTCGCCAGCGATAACCGGAGT4810260.4734613983425593No Hit
TAAAGTCATGCTGAATTGTTCGCGTTTACCTTGCGTGTACGCGCAGGAAA1647870.1621955641663347No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAAAGTC920400.077.123371
AAGTCAT1170500.060.7571953
AGTCATG1180600.060.3731734
AAAGTCA1264100.056.3601382
TCATGCG971500.055.5022666
GTCATGC1333000.053.327125
ATGCGGC1123800.045.822828
CATGCGG1174650.043.8002667
TGCGGCG1234850.041.9029929
GCGATAA687100.041.31254236-37
TAACCGG731950.038.56459440-41
CGATAAC687750.037.7698738-39
TAAGACG748850.037.62921564-65
ATAACCG688650.037.54115340-41
GATAACC772650.036.83133338-39
AGCGATA708850.036.7117636-37
CGGAGTA782600.035.4638544-45
GTAATAA798550.035.37740360-61
ATCTCGA819600.034.7201618-19
AATGGTA838550.033.6582656-57
Produced by FastQC (version 0.11.5)