FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005732536

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005732536
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences99859105
Sequences flagged as poor quality0
Sequence length101
%GC51

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AGTGATTAGCAGGCGTCCATCTCGAAGGAGTCGCCAGCGATAACCGGAGT1555390.15575845587640708No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GATTAGC365950.054.9353644
TGATTAG418350.048.2815363
GTGATTA494400.041.062782
ATTAGCA532600.037.4784435
GCGATAA247750.033.5005236-37
CGATAAC247400.033.10692238-39
ATAACCG255800.032.09358640-41
TAACCGG265050.031.2692240-41
AGCGATA265750.030.88292936-37
TAAGACG295550.028.24483964-65
TTAGCAG618100.027.853046
CGGAGTA305950.027.0963944-45
CGACCAA314200.026.2191270-71
TAGCAGG658800.024.6905237
AACCGGA340500.024.16595342-43
GAGTCGC346950.023.81621728-29
AGACGAC356650.023.61226566-67
AGTCGCC356900.023.38516228-29
ATCTCGA381750.022.03284818-19
GATAACC406350.020.78781738-39
Produced by FastQC (version 0.11.5)