FastQCFastQC Report
Wed 20 Apr 2022
EGAF00005732585

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005732585
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences102128489
Sequences flagged as poor quality0
Sequence length75
%GC50

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCCGATC259550.043.9309324
CGTATCA192250.043.90202349
CGCCGTA214150.042.97176446
CGGCATT346550.041.6666071
AACCGCT352800.037.36488315
CCGATCA258300.036.29281625
AGATCGG297850.036.02969431
TGAACCG438800.035.46563313
TTCCGAT384850.034.39501623
CTTCCGA385950.034.33257722
CGCTCTT389450.032.7150218
GATCGGA503200.031.4176031
CAGATCG284500.031.19564430
GCCGTAT313850.030.89236647
GGTCGCC393550.029.2773743
CCGCTCT445800.028.29036517
CCGTATC331750.028.05033948
TGGTCGC276000.027.13163842
TCGGAAG562350.027.0897243
CGTGTAG398750.025.59249715
Produced by FastQC (version 0.11.5)